FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC294CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC294CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21733
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTCGCACGCGTACGACGCACTCCCATTCGCTTCTGGTAGGCCTGCAA980.4509271614595316No Hit
CGAGTCGCACGCGTACGACGCACTAAGACCACTGTGGACCATATGGCCAT830.3819076979708278No Hit
CGAGTCGCACGCGTACGACGCACCGAGGCCAAGGGTGTTGCCTCTTCCTT380.17484930750471633No Hit
CGAGTCGCACGCGTACGACGCACCGGGGCCAAGGAGGTGTCACTAAGCTA380.17484930750471633No Hit
CGAGTCGCACGCGTACGACGCAAGAAGCTCAAAAGAGAAATAACGTTCCA380.17484930750471633No Hit
CGAGTCGCACGCGTACGACGCACGGAAACCACTGACTGTCCAGCAGCAAA350.16104541480697557No Hit
CGAGTCGCACGCGTACGACGCACGCAAAGAGGGCCTATTATCTTTTGCCT300.13803892697740763No Hit
CGAGTCGCACGCGTACGACGCAAAGAGAACACAATCTGGACTAGCGGGAG300.13803892697740763No Hit
CGAGTCGCACGCGTACGACGCACCCCCATTCGCTTCTGGTAGGCCTGCAA280.12883633184558047No Hit
CGAGTCGCACGCGTACGACGCAAGCATCAATGAACTGAGCAATCTTGCAA270.12423503427966685No Hit
CGAGTCGCACGCGTACGACGCAAGGGGACGTAGTGTTGGTAATGAAACGA260.11963373671375328No Hit
CGAGTCGCACGCGTACGACGCACGGGGGCCAAGGAGGTGTCACTAAGCTA240.11043114158192609No Hit
CGAGTCGCACGCGTACGACGCACCCCCCACGCGTGATCAGTAGAAACAAG240.11043114158192609No Hit
CGAGTCGCACGCGTACGACGCACGGAGGCCAAGGGTGTTGCCTCTTCCTT240.11043114158192609No Hit
CGAGTCGCACGCGTACGACGCACAGAGCAGAAATGAAAAGTGGCGAGAGC240.11043114158192609No Hit
CGAGTCGCACGCGTACGACGCAGGGGACAGAAATTTGAGGAAATAAGGTG230.10582984401601252No Hit
CGAGTCGCACGCGTACGACGCAATTGGGACAGAAATTTGAGGAAATAAGG220.10122854645009892No Hit
CGAGTCGCACGCGTACGACGCAGGGGCCCGAACAACATGGATAGAGCAGT220.10122854645009892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA21600.094.7777949
CGAGTCG21450.094.3292851
GAGTCGC21600.094.118092
GTCGCAC21550.094.116044
AGTCGCA21650.093.900733
TCGCACG21650.093.900735
GCACGCG22150.092.638847
CACGCGT22500.091.197798
CGCACGC22800.089.581166
TTTCTCG301.4593406E-647.4988588-89
TAAGTGC301.4593406E-647.4988562-63
ACGCAGT600.047.4988518-19
CCTAAGT301.4593406E-647.4988560-61
ACGCAAT600.047.4988518-19
CACCGCG301.4593406E-647.4988520-21
CCTGAAC301.4593406E-647.4988568-69
GCAGCGG301.4593406E-647.4988520-21
GCAGCCA301.4593406E-647.4988520-21
CACCCCC301.4593406E-647.4988520-21
TCTCGAT301.4593406E-647.4988590-91
Produced by FastQC (version 0.11.5)