FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC293CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC293CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGCGACATACGTCGTGTCAGGCCCCCTCAAAGCCGAGATCGCGCAG60.5576208178438662No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGTAGTTTTTTACTCTA50.4646840148698885No Hit
CGCGCGCGACATACGTCGTGTCCGTAGAAACAAGGTAGTTTTTTACTCTA50.4646840148698885No Hit
CGCGCGCGACATACGTCGTGTCAGGATATAGCGGGAGTTTTGTTCAGCAT50.4646840148698885No Hit
CGCGCGCGACATACGTCGTGTCCGCCCCCCTTCAATGAAGCCGGCAATGG40.37174721189591076No Hit
CGCGCGCGACATACGTCGTGTCCTACGCGTGATCAGCAAAAGCAGGTAGA40.37174721189591076No Hit
CGCGCGCGACATACGTCGTGTCCGGCCCCCTCAAAGCCGAGATCGCGCAG40.37174721189591076No Hit
CGCGCGCGACATACGTCGTGTCGAGGCCAAGGGTGTTGCCTCTTCCTTTT30.2788104089219331No Hit
CGCGCGCGACATACGTCGTGTCCGGCCCCCTCAAAGCCGAGATCGGAAGA30.2788104089219331No Hit
CGCGCGCGACATACGTCGTGTCCAGAGCAGAAATGAAAAGTGGCGAGAGC30.2788104089219331No Hit
CGCGCGCGACATACGTCGTGTCCAGAGGCGAATGGGAGTGCAGATGCAGC30.2788104089219331No Hit
CGCGCGCGACATACGTCGTGTCCGGGGGCCAAGGAGGTGTCACTAAGCTA30.2788104089219331No Hit
CGCGCGCGACATACGTCGTGTCAGGCCCCCTCAAAGCCGAGATCGGAAGA30.2788104089219331No Hit
CGCGCGCGACATACGTCGTGTCCGGGAGGGAAGAATATCAACAGGAACAG30.2788104089219331No Hit
CGCGCGCGACATACGTCGTGTCAGGCGCCCTCAAAGCCGAGATCGCGCAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCCGCGTGATCAGTAGAAACAAGGTAGTT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCTCCAAGGGATGTGCAGTTGCGACGGCAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGTACTTTTTTGGACAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCGGCGGGGCAAGGAGGTGTCACTAAGCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGTCGTTTTTAAACAAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCAGGGAGCAAATGGAGTAAAAGGATTTT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCCCACCAAAACATATGCATCTGCATTCT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCCCGGGGCAACATCTTCTCCTATTTCAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGCAAAAGCAGGGTGACAAAAACATAAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGGGGCCAAGGAGGTGTCACTAAGCTAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCACGCACCCTCAAAGCCGAGATCGCGCAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGGGAGCGAATGGGAGTGCAGATGCAGC20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCTAGGGAAGAATATCAACAGGAACAGCAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGTACAAACAAGGTAGTTTTTTACTCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCCCCCACGCGTGATCAGTAGAAACAAGG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCTGGACAGTGGTGAACAGTATATGTAAC20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGCAAAAGCAGGTGTTTAAAATGAATCC20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCGGGAGGGGAACAGTGACCACAGAAGCTG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGAACGCGAAGTAGGTACAGATGCAATT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGGCCAAAAGCATGGAATATGATGCCGT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCGGGGGGCCAAGGAGGTGTCACTAAGCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCGGGGGAACAAGGTCGTTTTTAAACAATT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCGGGCCCCCTCAAAGCCGAGATCGCGCAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGTAGCAACAAGGCAGTTTTTTACTCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGTAGAAACAAGGTACTTTTTTGGACAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCGGGGACAGAAATTTGAGGAAATAAGGTG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGCAAAAGCAGGTAGATATTTAAAGATG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGGAGAAACAAGGTAGTTTTTTACTCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGGCCCCCTTCAATGAAGCCGGCAATGG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGTAGAAACAAGGTCGTTTTTAAACAAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGGGGGGCAAGGAGGTGTCACTAAGCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGGGAGCAAATGGAGTAAAAGGATTTTC20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGGAGAAACAAGGCAGTTTTTTACTCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCGTACGCGTGATCAGTAGAAACAAGGTAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGTAGAAACAAGGCCGTTTTTAAACAAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCGCACAAAAACATATGCATCTGCATTCT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGAAGAAACAAGGTAGTTTTTTACTCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGGGAGGGAACTTCACCAATGGGACAGC20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCAGGGGCGAAGGAGGTGTCACTAAGCTA20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCACCAACTTGGGGGGTTTTCGGCTGAATC20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGCAGAAGCAGGTTGACAAAAACATAAT20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCAGTAGCAACAAGGTACTTTTTTGGACAG20.18587360594795538No Hit
CGCGCGCGACATACGTCGTGTCCATCACTTGGTCCATCGGTAATTATGGT20.18587360594795538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACA1000.095.05
GCGACAT950.095.06
GCGCGCG1000.095.02
GCGCGAC1000.095.04
GACATAC1000.095.08
ACATACG1000.095.09
CGCGCGC1000.095.01
CGCGCGA1000.095.03
CGACATA1000.095.07
GTGTCAG204.24539E-447.50000418-19
CGTGTCG204.24539E-447.50000416-17
GTGTCGG150.00895845247.518-19
GTGTCCG251.9715004E-547.518-19
CATCCAC150.00895845247.580-81
ACTCTGC150.00895845247.590-91
TCTGCTG150.00895845247.592-93
TACGTCG1000.047.512-13
ATACGTC1000.047.510-11
CGTGTCC457.4578566E-1147.516-17
GCACTCT150.00895845247.588-89
Produced by FastQC (version 0.11.5)