FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC292CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC292CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52413
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGAGTCTATATACGTCGAGGCCAAGGGTGTTGCCTCTTCCTTTTA4560.8700131646728865No Hit
CGAGATGAGTCTATATACGTCGCGGCCAAGGGTGTTGCCTCTTCCTTTTA2440.4655333600442639No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAAAATGGACGAAGGACAAGG2110.4025718810218839No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAAATGGAGTAAAAGGATTTT1210.23085875641539313No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAACAGTGACCACAGAAGCTG1150.2194112147749604No Hit
CGAGATGAGTCTATATACGTCGAGGCCCAGGGTGTTGCCTCTTCCTTTTA1020.1946082078873562No Hit
CGAGATGAGTCTATATACGTCGGGGGGCTATATGAAGCAATCGAGGAGTG920.17552897181996832No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAATAGTTGGAGAAATTTCAC890.16980520099975197No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAAGATTGGTCTTGTCTTTAG840.16026558296605803No Hit
CGAGATGAGTCTATATACGTCGAGGCCCATGCAACTGGCAAGTGCACCAG810.1545418121458417No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGAAAATGGACGAAGGACAAGGG800.1526338885391029No Hit
CGAGATGAGTCTATATACGTCGGGGCCAAGGGTGTTGCCTCTTCCTTTTA780.14881804132562532No Hit
CGAGATGAGTCTATATACGTCGGGGGGGACAAGATTGGTCTTGTCTTTAG720.1373704996851926No Hit
CGAGATGAGTCTATATACGTCGGGGGCAGAAATTTGAGGAAATAAGGTGG700.13355465247171502No Hit
CGAGATGAGTCTATATACGTCGGAGGGGGGAAAATGGACGAAGGACAAGG670.1278308816514987No Hit
CGAGATGAGTCTATATACGTCGGGGGGGCAAATGGAGTAAAAGGATTTTC660.1259229580447599No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAAGGAGGTGTCACTAAGCTA600.11447541640432718No Hit
CGAGATGAGTCTATATACGTCGGGGAGGGCAATTGGGACAGAAATTTGAG590.11256749279758838No Hit
CGAGATGAGTCTATATACGTCGCGGCACAGATTGAAAGCGACAGAGAATA580.1106595691908496No Hit
CGAGATGAGTCTATATACGTCGGGGGGCAGAAATTTGAGGAAATAAGGTG550.10493579837063324No Hit
CGAGATGAGTCTATATACGTCGGGGGACAGAAATTTGAGGAAATAAGGTG530.10111995115715568No Hit
CGAGATGAGTCTATATACGTCGCCCAGCATTAAGCATCAATGAACTGAGC530.10111995115715568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGT254.559788E-794.997134
AGCCTAT156.5832137E-494.997138
GGGTCTA254.559788E-794.997137
GCCTATA156.5832137E-494.997139
TGAGCCT156.5832137E-494.997136
GAGATGG254.559788E-794.997132
ATGAGCC156.5832137E-494.997135
CGAGATG51500.094.714881
GAGTCTA50950.094.1581047
GAGATGA51600.093.984572
GTCTATA51500.093.6136869
AGTCTAT51300.093.423118
TGAGTCT52250.092.088136
AGATGAG52850.091.7616653
ATGAGTC52600.091.294775
GATGAGT52650.091.117874
GAGGCTA550.086.361047
ATGGGTC301.3493991E-679.1642765
GGTCTAT301.3493991E-679.1642768
TGGGTCT301.3493991E-679.1642766
Produced by FastQC (version 0.11.5)