FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC263CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC263CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17235
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGTGGCACGATGAAACC1320.7658833768494343No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGAGCTTGAAAAGGAG1030.5976211198143313No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGTGACAAAAACATAA590.3423266608645199No Hit
CGTCGTACGATAGCACTAGTATCGCCGGGGAAGAAGTGGACGTTTGAGTG480.278503046127067No Hit
CGTCGTACGATAGCACTAGTATGGGGGACGAGAGCGAGACTTCATCTCCA380.22048157818392805No Hit
CGTCGTACGATAGCACTAGTATCGGCTCTAGATTAAAGATTTAGATGCAG370.21467943138961418No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGTTTATTTATTTAGG320.18566869741804468No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAGCTTCGTGCTGGAGAAAA320.18566869741804468No Hit
CGTCGTACGATAGCACTAGTATCGGCGCTGTCTTCGAAATGGATGGCACT270.1566579634464752No Hit
CGTCGTACGATAGCACTAGTATCGGCCCCATGATCCAAACACCTCCCACC230.1334493762692196No Hit
CGTCGTACGATAGCACTAGTATCGCCGCCCCTGGAAGGACAAATCTTCTG230.1334493762692196No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGCTAGGACAAGTGAT230.1334493762692196No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGATAGGGTGAAAAATG220.12764722947490573No Hit
CGTCGTACGATAGCACTAGTATCGGCGCCCCTGGAAGGACAAATCTTCTG220.12764722947490573No Hit
CGTCGTACGATAGCACTAGTATCCGCGTGATCAGCAAAAGCAGGTGGCAC210.12184508268059183No Hit
CGTCGTACGATAGCACTAGTATCAGCAAAAGCAGGTGGCACGATGAAACC200.11604293588627793No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAGTAGAAAAGAAAAGGGAG200.11604293588627793No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAATACCAATCAGCAAAAGG200.11604293588627793No Hit
CGTCGTACGATAGCACTAGTATCGGCACTCCCATTCGCTTCTGGTAGGCC190.11024078909196403No Hit
CGTCGTACGATAGCACTAGTATCGCCCCCATGATCCAAACACCTCCCACC190.11024078909196403No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGAAATGAAGCAGGGGGCTGCGC190.11024078909196403No Hit
CGTCGTACGATAGCACTAGTATGGGGGGCAAAAACATAATGGACTCCAAC190.11024078909196403No Hit
CGTCGTACGATAGCACTAGTATGGGGGGCGAGAGCGAGACTTCATCTCCA190.11024078909196403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA16950.095.000011
CGATAGC16700.095.08
TACGATA16750.095.06
CGTACGA16700.095.04
TCGTACG16750.095.03
ACGATAG16750.095.07
GTACGAT16750.095.05
GTCGTAC16800.094.999992
GATAGCA16800.094.999999
GTATGAG452.1282176E-1047.50000418-19
TATCGGG452.1282176E-1047.50000420-21
AGTATCG5300.047.50000418-19
AGCACTA16950.047.50000412-13
CTAGTAT16950.047.50000416-17
GCACTAG16950.047.50000412-13
TTGTCTT301.4501729E-647.594-95
TGGTCGC252.7697652E-547.538-39
ATGGGCG301.4501729E-647.520-21
ATGGGAG252.7697652E-547.520-21
TGCATAG301.4501729E-647.574-75
Produced by FastQC (version 0.11.5)