FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC262CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC262CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13644
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACAGCGAGTCAGCGTAGCCCCCCAAGATCGAAGGTTCCAGGTTCC630.46174142480211083No Hit
CGACGACAGCGAGTCAGCGTAGGGGGGCTATATGAAGCAATCGAGGAGTG370.2711814717091762No Hit
CGACGACAGCGAGTCAGCGTAGCGCCCCAAGATCGAAGGTTCCAGGTTCC240.1759014951627089No Hit
CGACGACAGCGAGTCAGCGTAGCACCACATTCCCTTATACTGGAGATCCT220.16124303723248312No Hit
CGACGACAGCGAGTCAGCGTAGAGTCCCGTTTTCGTTTCATTACCAACAC220.16124303723248312No Hit
CGACGACAGCGAGTCAGCGTAGGCCCCCAAGATCGAAGGTTCCAGGTTCC200.1465845793022574No Hit
CGACGACAGCGAGTCAGCGTAGACCCCCAAGATCGAAGGTTCCAGGTTCC200.1465845793022574No Hit
CGACGACAGCGAGTCAGCGTAGGGAACGGGAACAGGATACACCATGGACA190.13925535033714453No Hit
CGACGACAGCGAGTCAGCGTAGGGGGGGGGAAGGCTCTATTGGGAAAGTG190.13925535033714453No Hit
CGACGACAGCGAGTCAGCGTAGAAACAAGGTCGTTTTTAAACAATTCGAC180.13192612137203166No Hit
CGACGACAGCGAGTCAGCGTAGGGCAAGGGAACGTAGTGTTGGTAATGAA180.13192612137203166No Hit
CGACGACAGCGAGTCAGCGTAGAGGGGGCCTGACGGGATGATAGAAAGAA160.11726766344180592No Hit
CGACGACAGCGAGTCAGCGTAGAGACAAGGTCGTTTTTAAACAATTCGAC160.11726766344180592No Hit
CGACGACAGCGAGTCAGCGTAGCACCCCAAGATCGAAGGTTCCAGGTTCC160.11726766344180592No Hit
CGACGACAGCGAGTCAGCGTAGATGCCATAAGCACCACATTCCCTTATAC150.10993843447669306No Hit
CGACGACAGCGAGTCAGCGTAGGGAACAGGAACAGGATACACCATGGACA150.10993843447669306No Hit
CGACGACAGCGAGTCAGCGTAGAGCAGCATATCCTTTTGTGGTGTAAACA140.10260920551158018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTC13400.094.6455159
CGACGAC13400.094.291041
AGCGAGT13400.094.291048
CGACAGC13300.094.2857064
CAGCGAG13300.094.2857067
ACGACAG13450.093.940523
GACGACA13450.093.587362
ACAGCGA13650.092.216126
GACAGCG13650.092.216125
GTAGATG252.7542972E-547.518-19
TAGCGGC301.4388443E-647.520-21
GTAGAGG600.047.518-19
GTAGAGC750.047.518-19
GGTTGCT252.7542972E-547.532-33
AGCTCCA252.7542972E-547.590-91
CCAGTGC252.7542972E-547.594-95
TCCTAGC252.7542972E-547.586-87
CATCCTA252.7542972E-547.584-85
AGGGCGG252.7542972E-547.520-21
ATGGTAC252.7542972E-547.554-55
Produced by FastQC (version 0.11.5)