FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC258CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC258CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68223
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGACTCTGTCT4360.6390806619468508No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAGCAAGAGAG3950.578983627222491No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGAGTTGAACT3740.5482022191929408No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGAATCTCTGC2310.33859548832505165No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGGTGTTTTC2070.3034167362912801No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTTCAAAGGTC1880.2755668909312109No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTGAAAGATGT1820.266772202922768No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGCTAGGACAA1780.26090907758380605No Hit
CGTAGAGTATCTGAGTATACGCCCAACAAATAACTGATAATTCTCAACAG1570.2301276695542559No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGAGCTTGAAA1470.21546985620685108No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGGAAAGGAAATGTAATGCATTT1420.20814094953314866No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGTGACAAAAA1220.17882532283833896No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTTGATGTGCT1150.1685648534951556No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGAAGCCAACA1130.16563329082567463No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGAAGCTTCA1080.1583043841519722No Hit
CGTAGAGTATCTGAGTATACGCCGGCGCCAAACCTTATGTGAAATTGTAA1020.14950969614352932No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGTGTTTTTT1010.1480439148087888No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGTGATCTTCC880.12898875745716254No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGCAAGTGTGAGTGAATGTGTGT870.12752297612242205No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGATGATCTCCA850.12459141345294109No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGTCGTTTTTA830.12165985078346012No Hit
CGTAGAGTATCTGAGTATACGCCAGAGCAGAAATGAAAAGTGGCGAGAGC810.11872828811397916No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGGTGGTGGTCA760.11139938144027674No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGACCCTGGCAA730.10700203743605528No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAAGATTCTTA690.10113891209709334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTGA201.7327038E-594.9926768
TAGAGCA550.094.992673
TAGGGTA353.237801E-1094.992673
GTATCTT156.5930554E-494.992667
ATCTGCG156.5930554E-494.992669
AGTATCT65750.094.7759556
GTAGAGT65500.094.775132
GTATCTG65900.094.704377
TATCTGA65600.094.703068
ATCTGAG66950.094.567019
GAGTATC65900.094.560225
AGAGTAT65650.094.558584
CGTAGAG66850.094.4917761
TAGAGTA65900.094.271933
CATCTGA500.085.49348
GAGGATC850.083.817065
GTAGGGT409.349606E-1083.118592
GAGCATC650.080.378415
GTAGAGC700.074.637092
AGCATCT700.074.637096
Produced by FastQC (version 0.11.5)