FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC257CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC257CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20322
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATAGTCGTACGTATACATCCGGCCATCTGTTCCATAGCCTTTGCCGT1260.6200177147918512No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAACAGTGACCACAGAAGCTG1060.5216022045074303No Hit
CGCATAGTCGTACGTATACATCCGGCGCCCAATTAGCACATTAGCTTTCT900.44286979627989376No Hit
CGCATAGTCGTACGTATACATCCGGCGCAAAATGCCATAAGCACCACATT810.39858281665190437No Hit
CGCATAGTCGTACGTATACATCCGGAGCAGAAATGAAAAGTGGCGAGAGC660.3247711839385887No Hit
CGCATAGTCGTACGTATACATCGGGGGGGCAAGAGAAGAACCCCGCACTC640.31492963291014664No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAAGAGAAGAACCCCGCACTC600.29524653085326247No Hit
CGCATAGTCGTACGTATACATCAGGAGCTAATGGTAGTGCCACCATCCAG360.1771479185119575No Hit
CGCATAGTCGTACGTATACATCCGGCCCTAGACACCATGGCCTCCACCAT320.15746481645507332No Hit
CGCATAGTCGTACGTATACATCGGGGGAAGAATATCAACAGGAACAGCAG310.15254404094085228No Hit
CGCATAGTCGTACGTATACATCCGGCCCCTTGCCCAATTAGCACATTAGC300.14762326542663123No Hit
CGCATAGTCGTACGTATACATCCAGAGCAGAAATGAAAAGTGGCGAGAGC300.14762326542663123No Hit
CGCATAGTCGTACGTATACATCAGGCGCAAAATGCCATAAGCACCACATT300.14762326542663123No Hit
CGCATAGTCGTACGTATACATCAGGCGCCCAATTAGCACATTAGCTTTCT300.14762326542663123No Hit
CGCATAGTCGTACGTATACATCGGGGGGCAAGAGAAGAACCCCGCACTCA300.14762326542663123No Hit
CGCATAGTCGTACGTATACATCCAGCGCAAAATGCCATAAGCACCACATT270.1328609388839681No Hit
CGCATAGTCGTACGTATACATCAGTAGAAACAAGGTCGTTTTTAAACAAT260.12794016336974706No Hit
CGCATAGTCGTACGTATACATCAGGGGGGGAACAGTGACCACAGAAGCTG260.12794016336974706No Hit
CGCATAGTCGTACGTATACATCCAGCCATCTGTTCCATAGCCTTTGCCGT240.11809861234130499No Hit
CGCATAGTCGTACGTATACATCAGGCACTCCTTCCGTAGAAGGCCCTCTT240.11809861234130499No Hit
CGCATAGTCGTACGTATACATCGGGAGGGGAACAGTGACCACAGAAGCTG230.11317783682708396No Hit
CGCATAGTCGTACGTATACATCAGGCCCCCTCAAAGCCGAGATCGCGCAG230.11317783682708396No Hit
CGCATAGTCGTACGTATACATCCGGCGCCCAAGATCGAAGGTTCCAGGTT230.11317783682708396No Hit
CGCATAGTCGTACGTATACATCCGGGGGGGAACAGTGACCACAGAAGCTG220.10825706131286292No Hit
CGCATAGTCGTACGTATACATCCGCCGCCCAATTAGCACATTAGCTTTCT220.10825706131286292No Hit
CGCATAGTCGTACGTATACATCCGGCCAGACTCAAAGTCGACCCTGGCAT220.10825706131286292No Hit
CGCATAGTCGTACGTATACATCCCGCACTCAGAATGAAGTGGATGATGGC220.10825706131286292No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAAAGGAAATGTAATGCATTT220.10825706131286292No Hit
CGCATAGTCGTACGTATACATCCTGAGCAGAAATGAAAAGTGGCGAGAGC210.10333628579864186No Hit
CGCATAGTCGTACGTATACATCGGGGAGGCAAGAGAAGAACCCCGCACTC210.10333628579864186No Hit
CGCATAGTCGTACGTATACATCCCGCCAGGCCCCCTCAAAGCCGAGATCG210.10333628579864186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGG156.273751E-495.9353261
CGCGTAG156.273751E-495.9353261
CGCATAG19650.095.4471051
TGGTCGT156.526546E-494.990155
GTCGTAA156.526546E-494.990157
GGCGTAC156.526546E-494.990157
ATAGGCG156.526546E-494.990154
ATGGTCG156.526546E-494.990154
AGGCGTA156.526546E-494.990156
CGTAGTC156.526546E-494.990153
CATAGGC156.526546E-494.990153
CGTAAGT156.526546E-494.990159
TCGTAAG156.526546E-494.990158
GCGTAGT156.526546E-494.990152
GCGTACG156.526546E-494.990158
GTAGTCG156.526546E-494.990154
GCATAGG156.526546E-494.990152
GGTCGTA156.526546E-494.990156
TAGGCGT156.526546E-494.990155
TAGTCGT19550.094.74725
Produced by FastQC (version 0.11.5)