FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC248CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC248CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60735
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTCGTTT5160.8495924919733268No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCCAAATA4350.7162262286984441No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTCAAATA3710.6108504157405121No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGGTGACAA3490.574627480036223No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTAGTTT3420.5631020004939491No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTAGATAT2860.4708981641557586No Hit
CGATACAGCTACTACTACTCTACGGAGCAGAAATGAAAAGTGGCGAGAGC2470.40668477813451887No Hit
CGATACAGCTACTACTACTCTACTAACCATTAATCTTGCGTAGCAGAGGC2350.3869268132049066No Hit
CGATACAGCTACTACTACTCTACAGAGCAGAAATGAAAAGTGGCGAGAGC2330.3836338190499712No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCCGTTT2200.3622293570428912No Hit
CGATACAGCTACTACTACTCTACGGCAAAGGCTATGGAACAGATGGCTGG2060.33917839795834365No Hit
CGATACAGCTACTACTACTCTAACGCGAAGTAGGTACAGATGCAATTGTC1670.2749650119371038No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGGTGTTT1670.2749650119371038No Hit
CGATACAGCTACTACTACTCTAGGGGGGCTATATGAAGCAATCGAGGAGT1550.2552070470074916No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCAGATAT1430.2354490820778793No Hit
CGATACAGCTACTACTACTCTAATTCGCTTCTGGTAGGCCTGCAAATTTT1420.23380258500041162No Hit
CGATACAGCTACTACTACTCTACTGAGCAGAAATGAAAAGTGGCGAGAGC1400.23050959084547623No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCGACAAA1290.2123981229933317No Hit
CGATACAGCTACTACTACTCTAAGCCGAGATCGCGCAGAGACTGGAAAGT1170.19264015806371945No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTGACAAA1170.19264015806371945No Hit
CGATACAGCTACTACTACTCTAAGACCACTGTGGACCATATGGCCATAAT1140.18770066683131637No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCAGTTT1090.17946818144397794No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCGATCT990.16300321066930107No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTACTGAT980.16135671359183337No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCATTTT950.1564172223594303No Hit
CGATACAGCTACTACTACTCTACCCCGCACTCAGAATGAAGTGGATGATG950.1564172223594303No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTACTTT920.15147773112702725No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTTGATGT910.14983123404955956No Hit
CGATACAGCTACTACTACTCTACGGCCCCTTGCCCAATTAGCACATTAGC910.14983123404955956No Hit
CGATACAGCTACTACTACTCTACCTCCAGAGCAGAAATGAAAAGTGGCGA900.14818473697209186No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTTGACAA870.1432452457396888No Hit
CGATACAGCTACTACTACTCTAAGCAGAAATGAAAAGTGGCGAGAGCAAT840.13830575450728574No Hit
CGATACAGCTACTACTACTCTATGGGGCCAAGGAGGTGTCACTAAGCTAT830.13665925742981808No Hit
CGATACAGCTACTACTACTCTAAGGCCACTGTGGACCATATGGCCATAAT820.13501276035235038No Hit
CGATACAGCTACTACTACTCTATTGGGCAAGGGGACGTAGTGTTGGTAAT800.131719766197415No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCGTTTT690.11360829834527043No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTGATCT650.10702231003539969No Hit
CGATACAGCTACTACTACTCTAGGGGGGGGAAGCTTCGTGCTGGAGAAAA630.10372931588046432No Hit
CGATACAGCTACTACTACTCTAGCGGGAGTTTTGTTCAGCATCCAGAACT630.10372931588046432No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCGTTTAA620.10208281880299663No Hit
CGATACAGCTACTACTACTCTACGTCCCCTTGCCCAATTAGCACATTAGC610.10043632172552894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA156.5661623E-495.077491
GCTGCTA156.587732E-494.9991768
CAGCTGC156.587732E-494.9991766
CGATACA60100.094.20741
ACAGCTA60200.094.131255
ATACAGC60500.093.978523
CAGCTAC60300.093.9751366
AGCTACT60300.093.9751367
GCTACTA60350.093.897288
TACAGCT60300.093.896364
GATACAG60450.093.741952
AATACAG255.2353957E-575.9993442
CAATACA301.2861549E-463.3849951
GAGCTAC250.00499862556.9995086
ACAGCTG250.00499862556.9995085
AGCTGCT250.00499862556.9995087
GTGCAAA205.3692725E-447.49958858-59
CTAGACG550.047.49958820-21
AACGTAG452.2191671E-1047.49958830-31
CTATGCG700.047.49958820-21
Produced by FastQC (version 0.11.5)