FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC236CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC236CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12203
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTATACTCGCACGAGCGTCCAGAGCAGAAATGAAAAGTGGCGAGAGC470.385151192329755No Hit
CGCGTATACTCGCACGAGCGTCAGGAGCTAATGGTAGTGCCACCATCCAG440.3605670736704089No Hit
CGCGTATACTCGCACGAGCGTCCAACACCTTTTTGGATCTAAAAAGTACT420.34417766123084487No Hit
CGCGTATACTCGCACGAGCGTCCAGGGGGGAAGAATATCAACAGGAACAG340.2786200114725887No Hit
CGCGTATACTCGCACGAGCGTCCGTAGAAGGCCCTCTTTTCAAACCGTAT340.2786200114725887No Hit
CGCGTATACTCGCACGAGCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAG290.23764648037367858No Hit
CGCGTATACTCGCACGAGCGTCCAAGAGGGAAGAATATCAACAGGAACAG280.2294517741538966No Hit
CGCGTATACTCGCACGAGCGTCCAGGAGGGAAGAATATCAACAGGAACAG270.22125706793411454No Hit
CGCGTATACTCGCACGAGCGTCCGGAGCTAATGGTAGTGCCACCATCCAG260.21306236171433254No Hit
CGCGTATACTCGCACGAGCGTCCGGGGGGGAAGAATATCAACAGGAACAG250.2048676554945505No Hit
CGCGTATACTCGCACGAGCGTCATTGCAGCAAATATCATTGGGATCTTGC250.2048676554945505No Hit
CGCGTATACTCGCACGAGCGTCCGGGAGGGAAGAATATCAACAGGAACAG240.1966729492747685No Hit
CGCGTATACTCGCACGAGCGTCGGGGGAAGAATATCAACAGGAACAGCAG230.18847824305498648No Hit
CGCGTATACTCGCACGAGCGTCCGGGGAAGAATATCAACAGGAACAGCAG230.18847824305498648No Hit
CGCGTATACTCGCACGAGCGTCAGTAGAAACAAGGTCGTTTTTAAACAAT220.18028353683520446No Hit
CGCGTATACTCGCACGAGCGTCCGGAGCAGAAATGAAAAGTGGCGAGAGC190.1556994181758584No Hit
CGCGTATACTCGCACGAGCGTCGGGGCAGCAAATATCATTGGGATCTTGC190.1556994181758584No Hit
CGCGTATACTCGCACGAGCGTCAGTAGAAACAAGGTAGTTTTTTACTCTA190.1556994181758584No Hit
CGCGTATACTCGCACGAGCGTCAGGATATAGCGGGAGTTTTGTTCAGCAT180.14750471195607637No Hit
CGCGTATACTCGCACGAGCGTCGGGAGCTAATGGTAGTGCCACCATCCAG180.14750471195607637No Hit
CGCGTATACTCGCACGAGCGTCGGGGGGCCAAGGAGGTGTCACTAAGCTA180.14750471195607637No Hit
CGCGTATACTCGCACGAGCGTCCATGAGGGAAGAATATCAACAGGAACAG170.13931000573629435No Hit
CGCGTATACTCGCACGAGCGTCCGGGACAGAAATTTGAGGAAATAAGGTG170.13931000573629435No Hit
CGCGTATACTCGCACGAGCGTCATCAGCAAAAGCAGGTAGATATTTAAAG160.13111529951651235No Hit
CGCGTATACTCGCACGAGCGTCCTGAGAGGATTTCTCATTTTAGGCAAAG150.12292059329673032No Hit
CGCGTATACTCGCACGAGCGTCAGGAAGGCAAGAGAAGAACCCCGCACTC150.12292059329673032No Hit
CGCGTATACTCGCACGAGCGTCGAGGGAAGAATATCAACAGGAACAGCAG150.12292059329673032No Hit
CGCGTATACTCGCACGAGCGTCATCAGCAAAAGCAGGTCAAATATATTCA150.12292059329673032No Hit
CGCGTATACTCGCACGAGCGTCATGAACTGAGCAATCTTGCAAAAGGAGA150.12292059329673032No Hit
CGCGTATACTCGCACGAGCGTCCGACACCTTTTTGGATCTAAAAAGTACT150.12292059329673032No Hit
CGCGTATACTCGCACGAGCGTCGGGACAGAAATTTGAGGAAATAAGGTGG140.1147258870769483No Hit
CGCGTATACTCGCACGAGCGTCGGGCCCCCTCAAAGCCGAGATCGCGCAG130.10653118085716627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT11950.095.776861
CGTATAC12050.094.9918063
GCGTATA12050.094.9918062
TACTCGC12050.094.9918067
CTCGCAC12050.094.9918069
ACTCGCA12100.094.99188
TATACTC12000.094.99185
ATACTCG12000.094.99186
GTATACT12000.094.5964
AGCGTCA3100.047.49590716-17
CGTCAGA252.7472459E-547.49590318-19
CGTCAAC252.7472459E-547.49590318-19
GTCCGTA252.7472459E-547.49590320-21
CACGAGC12150.047.49590312-13
AGCAGAA252.7472459E-547.49590326-27
GCAAATA252.7472459E-547.49590328-29
ACGAGCG12150.047.49590314-15
AAGGCCC252.7472459E-547.49590328-29
GCGTCTG252.7472459E-547.49590318-19
GCGTCCG1750.047.49590318-19
Produced by FastQC (version 0.11.5)