FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC219CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC219CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233722
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGCGCACTCGACTCGAGATCGGAAGAGCACACGTCTGAACTCCAG39651.6964598968004723Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGTCGCGCACTCGACTCGACAACAGCATAAGAATTGGCTCCAAAGGAG16920.7239369849650439No Hit
CGTGTCGCGCACTCGACTCGACCCAGAAGCAAGGTCTTATACAGTCCAAT13480.5767535790383447No Hit
CGTGTCGCGCACTCGACTCGACAGGCCCCCTCAAAGCCGAGATCGCACAG10130.4334209017550766No Hit
CGTGTCGCGCACTCGACTCGACATGAGAACAGAATGGTTCTGGCCAGCAC9000.3850728643431085No Hit
CGTGTCGCGCACTCGACTCGACGCGTGATCAGTAGAAACAAGGTAGTTTT7890.33758054440745844No Hit
CGTGTCGCGCACTCGACTCGACGCGTGATCAGTAGAAACAAGGGTGTTTT7030.30078469292578364No Hit
CGTGTCGCGCACTCGACTCGACAGCAGGGGAAAATAAAAACAACCAGAAT6580.2815310497086282No Hit
CGTGTCGCGCACTCGACTCGACGGCAGGGGAAAATAAAAACAACCAGAAT6550.2802474734941512No Hit
CGTGTCGCGCACTCGACTCGACAGCACCTGGGTGAATCACACATATGTTA6270.2682674288256989No Hit
CGTGTCGCGCACTCGACTCGACGGGGGGCAAAGACATAATGGATTCCCAC5970.25543166668092865No Hit
CGTGTCGCGCACTCGACTCGACCCAGCCATTTGCTCCATAGCCTTAGCTG5140.21991939141373082No Hit
CGTGTCGCGCACTCGACTCGACAGGCAAATGGTAACAACAACCAATCCAT5120.2190636739374128No Hit
CGTGTCGCGCACTCGACTCGACTGCAGATGTATCCTATTTGATAATCCAA5000.21392936907950472No Hit
CGTGTCGCGCACTCGACTCGACCTACGCGTGATCAGTAGAAACAAGGGTG4770.2040886181018475No Hit
CGTGTCGCGCACTCGACTCGACCAGCCATTTGCTCCATAGCCTTAGCTGT4370.18697426857548713No Hit
CGTGTCGCGCACTCGACTCGACGCGTGATCAGTAGAAACAAGGGTATTTT4290.18355139867021503No Hit
CGTGTCGCGCACTCGACTCGACAAGACCAATCCTGTCACCTCTGACTAAG4200.17970067002678397No Hit
CGTGTCGCGCACTCGACTCGACAATCCCAATTATACTTGCGGCAACAACA4190.17927281128862496No Hit
CGTGTCGCGCACTCGACTCGACAGGGGGCAAAGACATAATGGATTCCCAC4180.17884495255046595No Hit
CGTGTCGCGCACTCGACTCGACCAGCGCGGGGAAAGATCCTAAGAAAACT4170.17841709381230694No Hit
CGTGTCGCGCACTCGACTCGACGCAGGGGAAAATAAAAACAACCAGAATG4060.1737106476925578No Hit
CGTGTCGCGCACTCGACTCGACATCCAAATGTGCACCGAGCTTAAGCTCA4050.1732827889543988No Hit
CGTGTCGCGCACTCGACTCGACCCCCCACGCGTGATCAGTAGAAACAAGG4030.17242707147808078No Hit
CGTGTCGCGCACTCGACTCGACCTACGCGTGATCAGTAGAAACAAGGTAG4020.1719992127399218No Hit
CGTGTCGCGCACTCGACTCGACAGAAACGAAAACGTTCTAATCTCTTGTT3980.17028777778728574No Hit
CGTGTCGCGCACTCGACTCGACGCGTGATCAGCAAAAGCAGGTAGATATT3940.16857634283464973No Hit
CGTGTCGCGCACTCGACTCGACGCGTGATCAGCGAAAGCAGGTAGATATT3930.16814848409649072No Hit
CGTGTCGCGCACTCGACTCGACCTTGGGTGTCTTTTAATCAAAACTTGGA3910.16729276662017267No Hit
CGTGTCGCGCACTCGACTCGACCCCCTACGCGTGATCAGTAGAAACAAGG3890.16643704914385465No Hit
CGTGTCGCGCACTCGACTCGACAAGCCAACAATGGTGATGATGCAACGGC3700.15830773311883348No Hit
CGTGTCGCGCACTCGACTCGACTGAAACGAAAACGTTCTAATCTCTTGTT3700.15830773311883348No Hit
CGTGTCGCGCACTCGACTCGACAGGAGAAAAACCCATCACTTAGGATGAA3600.15402914573724338No Hit
CGTGTCGCGCACTCGACTCGACAGAAGCAAGGTCTTATACAGTCCAATCC3510.1501784170938123No Hit
CGTGTCGCGCACTCGACTCGACCCTCCAGAAGTTTCGGTCATTGATCCCA3340.14290481854510914No Hit
CGTGTCGCGCACTCGACTCGACCTACGCGTGATCAGCAAAAGCAGGTAGA3250.13905408990167806No Hit
CGTGTCGCGCACTCGACTCGACCGAGAGCTCAAAGACTCCCCGCCCCTGG3230.13819837242536004No Hit
CGTGTCGCGCACTCGACTCGACCAACCCAGAAGCAAGGTCTTATACAGTC3060.13092477387665688No Hit
CGTGTCGCGCACTCGACTCGACCTACGCGTGATCAGCGAAAGCAGGTAGA2970.1270740452332258No Hit
CGTGTCGCGCACTCGACTCGACAAAAGCAGGTAGATATTGAAAGATGAGT2940.12579046901874877No Hit
CGTGTCGCGCACTCGACTCGACATCCCAGCGCGGGGAAAGATCCTAAGAA2890.12365117532795374No Hit
CGTGTCGCGCACTCGACTCGACCTCATAGACTCTGGTACTCCTTCCGTAG2880.12322331658979471No Hit
CGTGTCGCGCACTCGACTCGACCGAAACTTCTGGAGGGGTGAGAATGGAA2870.1227954578516357No Hit
CGTGTCGCGCACTCGACTCGACGAAAACGTTCTAATCTCTTGTTCCACTT2850.12193974037531768No Hit
CGTGTCGCGCACTCGACTCGACGGGGGGAGAATGGAAGAAAAACAAGGAT2850.12193974037531768No Hit
CGTGTCGCGCACTCGACTCGACAACCCAGAAGCAAGGTCTTATACAGTCC2820.12065616416084064No Hit
CGTGTCGCGCACTCGACTCGACAACACAGAAACGAAAACGTTCTAATCTC2740.11723329425556857No Hit
CGTGTCGCGCACTCGACTCGACGGGGAGGGAACTCGTCCTTTATGACAAA2630.11252684813581948No Hit
CGTGTCGCGCACTCGACTCGACATTCCATGGGGCCAAAGAAATAGCACTC2620.11209898939766047No Hit
CGTGTCGCGCACTCGACTCGACCGGGGGCAAAGACATAATGGATTCCCAC2540.1086761194923884No Hit
CGTGTCGCGCACTCGACTCGACCAAAGAATCATCACATATGAAAACAGCA2520.10782040201607038No Hit
CGTGTCGCGCACTCGACTCGACAGCCGGGGAAAGATCCTAAGAAAACTGG2510.10739254327791137No Hit
CGTGTCGCGCACTCGACTCGACAAGGGTGTTTTTTATCACTAAATAAGCT2480.10610896706343433No Hit
CGTGTCGCGCACTCGACTCGACAGCATAAGAATTGGCTCCAAAGGAGATG2390.10225823842000324No Hit
CGTGTCGCGCACTCGACTCGACAAAAGGGGAAGGTTACTAAATCAATAGA2390.10225823842000324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTCG254.5355955E-795.25661
CGTATCG201.7177414E-595.25661
CGTGCCG201.7177414E-595.25661
CATGTCG254.5355955E-795.25661
CGTGTAG201.7177414E-595.25661
TCACGCA201.7416707E-594.991665
TAGCGCA201.7416707E-594.991665
GTAGCGC201.7416707E-594.991664
TATCGCG201.7416707E-594.991663
TGCCGCG201.7416707E-594.991663
GTGCCGC201.7416707E-594.991662
GTCGCTC156.613582E-494.991654
GTTTCGC156.613582E-494.991652
GCGCGCT301.2283635E-894.991657
GCGCGCA156.613582E-494.991655
TTTCGCG156.613582E-494.991653
TGGCGCG156.613582E-494.991653
CGCGCTC301.2283635E-894.991658
CGCGCGC301.2283635E-894.991656
CGCGCAA156.613582E-494.991656
Produced by FastQC (version 0.11.5)