FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC216CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC216CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99042
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTGTGATAGTATCTCGTAGATCAGCAAAAGCAGGTAGATATTGAAAG7990.806728458633711No Hit
CGAGTGTGATAGTATCTCGTAGAAGGCCCTCTTTTTAAACCGTGTTTAAA3070.3099695078855435No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGTAGTTTTTTACTCTAGCTCT3070.3099695078855435No Hit
CGAGTGTGATAGTATCTCGTAGATCAGCAAAAGCAGGCAGATATTGAAAG2670.26958260132065187No Hit
CGAGTGTGATAGTATCTCGTAGCCGCAAGTATAATTGGGATTGTGCACTT2330.2352537307404939No Hit
CGAGTGTGATAGTATCTCGTAGCGAACAAGCAGCTGAGGCCATGGAGGTT2120.2140506047939258No Hit
CGAGTGTGATAGTATCTCGTAGATATTGAAAGATGAGTCTTCTAACCGAG1990.20092486016033603No Hit
CGAGTGTGATAGTATCTCGTAGACTCTGGTACTCCTTCCGTAGAAGGCCC1810.18275075220613476No Hit
CGAGTGTGATAGTATCTCGTAGGGGGCGGGAACAACTAGGTCAGAAGTTT1780.1797217342137679No Hit
CGAGTGTGATAGTATCTCGTAGAAGCAAGGTAGTTTTTTACTCTAGCTCT1760.1777023888855233No Hit
CGAGTGTGATAGTATCTCGTAGCGCCACAAAAAGAAATGCTGCTCCCACT1700.17164435290078955No Hit
CGAGTGTGATAGTATCTCGTAGCCACAAAAAGAAATGCTGCTCCCACTAG1690.17063468023666728No Hit
CGAGTGTGATAGTATCTCGTAGACGCCACAAAAAGAAATGCTGCTCCCAC1680.16962500757254498No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGTCGTTTTTAAATTATTCGAA1500.15145089961834374No Hit
CGAGTGTGATAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1470.14842188162597686Illumina PCR Primer Index 6 (96% over 27bp)
CGAGTGTGATAGTATCTCGTAGCGGCAACAACAAGAGGATCACTTGAATC1400.14135417297712083No Hit
CGAGTGTGATAGTATCTCGTAGTTTTTTACTCTAGCTCTATGCTGACAAA1390.14034450031299853No Hit
CGAGTGTGATAGTATCTCGTAGAGGCCCTCTTTTTAAACCGTGTTTAAAG1340.13529613699238704No Hit
CGAGTGTGATAGTATCTCGTAGGGGGGGCAAATGCAACGATTCAAGTGAT1310.1322671190000202No Hit
CGAGTGTGATAGTATCTCGTAGGGGGCAGGAATGAGAATACTTGTGAGGG1310.1322671190000202No Hit
CGAGTGTGATAGTATCTCGTAGAAAGCAGGAAGATATTGAAAGATGAGTC1260.12721875567940874No Hit
CGAGTGTGATAGTATCTCGTAGCCAAACTGGAAGTCAAAACAACACTGGA1260.12721875567940874No Hit
CGAGTGTGATAGTATCTCGTAGGAAGGGGCAAAAGTAACAGACTTAGAAA1230.12418973768704186No Hit
CGAGTGTGATAGTATCTCGTAGCAAAGACATAATGGATTCCCACACTGTG1220.12318006502291957No Hit
CGAGTGTGATAGTATCTCGTAGCAGCCCTCTGACTAACTCAACCCAGAAG1210.12217039235879729No Hit
CGAGTGTGATAGTATCTCGTAGATCAGCAAAAGCAGGCGTTTAAAATGAA1200.12116071969467498No Hit
CGAGTGTGATAGTATCTCGTAGAGTCCCGTTTTCGTTTCATTACCAACAC1180.11914137436643041No Hit
CGAGTGTGATAGTATCTCGTAGGGGGGGCAAATGCAACAACAAGAGGATC1130.11409301104581895No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGCAGTTTTTTACTCTAGCTCT1130.11409301104581895No Hit
CGAGTGTGATAGTATCTCGTAGCAGGCGGGAACAACTAGGTCAGAAGTTT1050.10601562973284061No Hit
CGAGTGTGATAGTATCTCGTAGAACCCAAATCAAAAGATAATAACCATTG1040.10500595706871832No Hit
CGAGTGTGATAGTATCTCGTAGAAGCAAGGTCGTTTTTAAATTATTCGAA1030.10399628440459603No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGGTGTTTTTTATCACTAAATA1030.10399628440459603No Hit
CGAGTGTGATAGTATCTCGTAGCAGGGGGCAAAGACATAATGGATTCCCA1020.10298661174047373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG201.714844E-595.2339251
TGAGTGT409.094947E-1295.2339251
TGATAGC254.5912202E-794.993447
GTTTGAT156.6017325E-494.993434
TGCGATA156.6017325E-494.993435
GCGATAG156.6017325E-494.993436
GAGTTTG156.6017325E-494.993432
ATAGTGT201.7365295E-594.993439
GTGGTAG201.7365295E-594.993436
CGATAGT156.6017325E-494.993437
GGTAGTA201.7365295E-594.993438
GGGATAG201.7365295E-594.993436
CGAGTGT96300.094.937251
TGTGATA96650.094.846015
GTGATAG97050.094.7976766
TGATAGT96750.094.747977
ATAGTAT97250.094.6515669
GATAGTA97100.094.651038
AGTGTGA97000.094.503783
GTGTGAT97150.094.4067464
Produced by FastQC (version 0.11.5)