FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC193CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC193CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455044
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGTATAGACGTGATACTGTGTCCATGGACACAGTAAACAGAACACA38190.8392595001802023No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGAACAGGAACAGGATACACC31820.6992730373326536No Hit
CGTATGTATAGACGTGATACTGAAACGAGAAAGCTCTTATCTCTTGTTCT27810.6111496910188905No Hit
CGTATGTATAGACGTGATACTGGACCACAACTGCCTGTCTTATCATTAGG27560.605655716809803No Hit
CGTATGTATAGACGTGATACTGAAGAGCTTCCCAGCACTGGGATGCTCTT24400.5362118828069373No Hit
CGTATGTATAGACGTGATACTGGCGGGGAGCGCCAGGATGCCACAGAAAT22960.5045665913625935No Hit
CGTATGTATAGACGTGATACTGAGGCCTATTCAACTGGTGCACTTGCCAG18720.41138878877646995No Hit
CGTATGTATAGACGTGATACTGAAAAGGGAAAGATAGTCAAATCAGTCGA15330.3368904985012438No Hit
CGTATGTATAGACGTGATACTGAAGATCTTGTATGAGGCCTGTCCATCAC13270.2916201510183631No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTAGATATTTAAAGATGAGT12470.2740394335492832No Hit
CGTATGTATAGACGTGATACTGAGGAGCTAAGAGAGCAATTGAGCTCAGT11900.2615131723525637No Hit
CGTATGTATAGACGTGATACTGGGGGGGGGAAAATGGACGAAGGACAAGG11610.25514016227002223No Hit
CGTATGTATAGACGTGATACTGGTGGGGAGCGCCAGGATGCCACAGAAAT10890.23931751654785033No Hit
CGTATGTATAGACGTGATACTGGGGGGGAGCGCCAGGATGCCACAGAAAT10820.23777920376930584No Hit
CGTATGTATAGACGTGATACTGTTTTCTTAGGGTCCTTCCCAGCACTGGG9970.2190996914584084No Hit
CGTATGTATAGACGTGATACTGTTACATTCTTTTCTAGTACTGTGTCTAC9480.20833150200859699No Hit
CGTATGTATAGACGTGATACTGGAGGGGGGAAAATGGACGAAGGACAAGG9100.199980661210784No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGCATTTTTTCATGAAGGAC9060.19910162533733003No Hit
CGTATGTATAGACGTGATACTGAAACAAGGCATTTTTTCATGAAGGACAA8960.19690403565369502No Hit
CGTATGTATAGACGTGATACTGCAAGCCCATACACACAAGCAGGCAGGCA8870.19492620493842355No Hit
CGTATGTATAGACGTGATACTGAAGCGAATGGGAGTGCAGATGCAGCGAT8740.19206933834969805No Hit
CGTATGTATAGACGTGATACTGAACAAAACTCCCGCTATATCCTGACCAC8180.1797628361213421No Hit
CGTATGTATAGACGTGATACTGATTCCCAAGTTGAATTGAGTGGCTAATC7910.17382934397552763No Hit
CGTATGTATAGACGTGATACTGATGCAATTGTCGTTCTAAGTGTCTCGCT7820.17185151326025613No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAGAGGATGGTGC7620.16745633389298617No Hit
CGTATGTATAGACGTGATACTGCAGAGACTGGAAAGTGTCTTTGCAGGAA7510.16503898524098767No Hit
CGTATGTATAGACGTGATACTGGGAGGCTGGTGTTTATAGCACCCTTGGG7400.1626216365889892No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGGTGACAAAAACATAATGGA7260.15954501103190022No Hit
CGTATGTATAGACGTGATACTGATTGCCCCCAGGGAGACTACCAGTACCA7130.15668814444317472No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGTAGATATTTAAAGATGAGT7130.15668814444317472No Hit
CGTATGTATAGACGTGATACTGCGCAGAGACTGGAAAGTGTCTTTGCAGG7100.15602886753808423No Hit
CGTATGTATAGACGTGATACTGATCCAAAATGGAAGACTTTGTGCGACAA6940.15251272404426824No Hit
CGTATGTATAGACGTGATACTGTTTACACCACAAAAGGATATGCTGCTCC6790.14921633951881577No Hit
CGTATGTATAGACGTGATACTGGAAAAGGGAAAGATAGTCAAATCAGTCG6650.14613971396172679No Hit
CGTATGTATAGACGTGATACTGAGGCTCTCATGGAATGGCTAAAGACAAG6420.1410852576893663No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGCGTTTAAAATGAATCCAAA6160.13537152451191534No Hit
CGTATGTATAGACGTGATACTGGACGGATCATGATCTCAGAGAACTCTTC6100.13405297070173433No Hit
CGTATGTATAGACGTGATACTGAAGAGCACACAGAATGCCATTGACGAGA6040.13273441689155335No Hit
CGTATGTATAGACGTGATACTGAAAAGGGGAAGATAGTCAAATCAGTCGA6010.13207513998646284No Hit
CGTATGTATAGACGTGATACTGATCAGCAAAAGCAGGTAGATATTTAAAG5790.12724044268246587No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGAAAGCACCATCCTCTCTATT5650.1241638171253769No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGAGTTTAAAATGAATCCAAA5630.12372429918864988No Hit
CGTATGTATAGACGTGATACTGAAACAAGGTAGTTTTTTACTCTAGCTCT5580.12262550434683239No Hit
CGTATGTATAGACGTGATACTGGCGGGGGGCGCCAGGATGCCACAGAAAT5570.1224057453784689No Hit
CGTATGTATAGACGTGATACTGTTGTGGTCCAGTATCGTCTAATGGAGCA5560.1221859864101054No Hit
CGTATGTATAGACGTGATACTGATCCCCTCAGAATGAGTGCTGACCGTGC5340.11735128910610841No Hit
CGTATGTATAGACGTGATACTGACCATCGTCAACATCCACAGCACTCTGC5330.11713153013774492No Hit
CGTATGTATAGACGTGATACTGGGGGGGGAAAATGGACGAAGGACAAGGG5270.11581297632756392No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTCAAATATATTCAATATGG5180.11383514561229242No Hit
CGTATGTATAGACGTGATACTGATTAGCAACCTCCATGGCCTCTGCTGCC5120.11251659180211145No Hit
CGTATGTATAGACGTGATACTGAAATGGGAGGCTGGTGTTTATAGCACCC5090.11185731489702096No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAAAGCACCATCC5030.11053876108683995No Hit
CGTATGTATAGACGTGATACTGGAAATCTAGTGGTACCGAGATATGCATT4960.10900044830829546No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTAAACCATTTGAATGGATG4930.10834117140320497No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGGTGACAAAAACATAATGGA4890.10746213552975098No Hit
CGTATGTATAGACGTGATACTGAAGAAACAAGGCATTTTTTCATGAAGGA4850.10658309965629698No Hit
CGTATGTATAGACGTGATACTGAACACACCAATACTCAGAAAAGAACAGA4830.10614358171956997No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGTAGTTTTTTACTCTAGCT4830.10614358171956997No Hit
CGTATGTATAGACGTGATACTGAGCAGAAATGAAAAGTGGCGAGAGCAAT4750.10438550997266198No Hit
CGTATGTATAGACGTGATACTGAGGAGATTTCATCGATTATGAGGAGCTA4660.1024076792573905No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGAGTTTAAAATGAATCCAAA4630.10174840235229998No Hit
CGTATGTATAGACGTGATACTGCAGCGATTCAAGTGATCCTCTCGTCATT4630.10174840235229998No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGGCGTTTAAAATGAATCCAAA4620.10152864338393651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA450.095.002976
TATAGTC156.614513E-495.002977
TCGACGT254.6152854E-795.002979
TATCGAC201.7424221E-595.002977
TACACGT201.7424221E-595.002979
CGTATAG353.2923708E-1095.002965
GTACGTA353.2923708E-1094.992522
TACGTAT353.2923708E-1094.992523
TAGACGT444600.094.853399
GTATAGA446600.094.843426
TATAGAC445750.094.82187
CGTATGT449450.094.6715551
ATAGACG446950.094.5991068
TGTATAG448300.094.536755
GTATGTA450350.094.380832
ATGTATA450600.094.043874
TATGTAT458950.092.477753
CGTATGG550.086.58521
TGGACGT1000.085.502689
ATAGAGG1000.085.502688
Produced by FastQC (version 0.11.5)