FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC179CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC179CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30818
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGACGTACAGCGCGTGTAAGTAGAAACAAGGTAGTTTTTTACTCTA1120.36342397300279056No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGGTGTTT830.2693231228502823No Hit
CGCGTGACGTACAGCGCGTGTAAGGCTTGCATAAATGTTATTTGTTCGAA680.22065026932312284No Hit
CGCGTGACGTACAGCGCGTGTAATGACCGATGGACCAAGTGATGGACAGG560.18171198650139528No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGAGTTTAAAATGAA540.17522227269777402No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGCGTTTAAAATGAA540.17522227269777402No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGTAGTTT510.16548770199234214No Hit
CGCGTGACGTACAGCGCGTGTAAAGCGAATCCAAACCAAAAGATAATAAC440.14277370367966774No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGGTGACAAAAACAT440.14277370367966774No Hit
CGCGTGACGTACAGCGCGTGTAAGCAAAAGCAGGCGTTTAAAATGAATCC430.1395288467778571No Hit
CGCGTGACGTACAGCGCGTGTAGGGTGACAAAAACATAATGGACTCCAAC420.13628398987604648No Hit
CGCGTGACGTACAGCGCGTGTAATCAGCAAAAGCAGGTAGATATTTAAAG410.13303913297423584No Hit
CGCGTGACGTACAGCGCGTGTATGCTAGAGTCCCGTTTTCGTTTCATTAC410.13303913297423584No Hit
CGCGTGACGTACAGCGCGTGTACAGCAAAAGCAGGCGTTTAAAATGAATC380.12330456226880394No Hit
CGCGTGACGTACAGCGCGTGTACAAAAACATAATGGACTCCAACACCATG380.12330456226880394No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGACAAAAACATAATGGACTCCAAC370.12005970536699331No Hit
CGCGTGACGTACAGCGCGTGTAAATGGCAACTCAGCACCGTCTGGCCAAG360.11681484846518268No Hit
CGCGTGACGTACAGCGCGTGTAAGCAGGGGAAAACAAAAGCAACAAAAAT360.11681484846518268No Hit
CGCGTGACGTACAGCGCGTGTACAGAGGGCAACCCACAGTGTCACTGTTT360.11681484846518268No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGCAAAAACATAATGGACTCCAAC340.11032513466156142No Hit
CGCGTGACGTACAGCGCGTGTAAGCAAAAGCAGGAGTTTAAAATGAATCC340.11032513466156142No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGCGTTTT330.10708027775975079No Hit
CGCGTGACGTACAGCGCGTGTAAAGACAACAGTATAAGAATCGGTTCCAA330.10708027775975079No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGTGGACAGGGATGGTAGATGGAT330.10708027775975079No Hit
CGCGTGACGTACAGCGCGTGTACAGCAAAAGCAGGAGTTTAAAATGAATC330.10708027775975079No Hit
CGCGTGACGTACAGCGCGTGTAAAAACATAATGGACTCCAACACCATGTC320.10383542085794016No Hit
CGCGTGACGTACAGCGCGTGTACGCGTGATCAGCAAAAGCAGGCGTTTAA320.10383542085794016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAC30200.094.995132
CGTGACG30200.094.995133
GTGACGT30150.094.9951254
TGACGTA30150.094.9951255
ACGTACA30300.094.838377
CGTACAG30300.094.838378
GACGTAC30250.094.838126
GTACAGC31050.092.700569
CGCGTGA31150.092.089051
GTAGAGG650.047.49756620-21
CCGGAGA205.3496746E-447.49756624-25
TAGGAGG404.114554E-947.49756620-21
TAGGAGA357.761446E-847.49756620-21
GTGTATG550.047.49756618-19
AGGATGC205.3496746E-447.49756670-71
GTACCGG205.3496746E-447.49756620-21
TAAGCGG205.3496746E-447.49756620-21
GTGTAGC1700.047.49756618-19
GTACTGC205.3496746E-447.49756620-21
GTGTAGA3200.047.49756618-19
Produced by FastQC (version 0.11.5)