FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC160CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC160CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59363
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGACGTACACTCTAGTATCCTGACCACTCATTTATTCCTACGATATC4090.6889813520206188No Hit
CGCTGACGTACACTCTAGTATCCTACGATATCTTGCTTTATTGAGAAGTT3890.6552903323619089No Hit
CGCTGACGTACACTCTAGTATCCAGAGCAGAAATGAAAAGTGGCGAGAGC2580.4346141535973586No Hit
CGCTGACGTACACTCTAGTATCGGGGGGCAAATGGAGTAAAAGGATTTTC2460.4143995418021326No Hit
CGCTGACGTACACTCTAGTATCGGGGGAGCAAATGGAGTAAAAGGATTTT2230.37565486919461616No Hit
CGCTGACGTACACTCTAGTATCATGCGAACAATTCAACAGACACTGTAGA2180.3672321142799387No Hit
CGCTGACGTACACTCTAGTATCCTGCGATATCTTGCTTTATTGAGAAGTT2070.3487020534676482No Hit
CGCTGACGTACACTCTAGTATCCTGAGCACTCATTTATTCCTACGATATC1920.3234337887236157No Hit
CGCTGACGTACACTCTAGTATCCCGACCAGTGAGTACCCTTCCCTTTCAA1670.2813200141502283No Hit
CGCTGACGTACACTCTAGTATCATGACCACTCATTTATTCCTACGATATC1620.27289725923555075No Hit
CGCTGACGTACACTCTAGTATCGGGGAGCAAATGGAGTAAAAGGATTTTC1620.27289725923555075No Hit
CGCTGACGTACACTCTAGTATCATTCGCTTCTGGTAGGCCTGCAAATTTT1610.27121270825261523No Hit
CGCTGACGTACACTCTAGTATCAGGCACTCCTTCCGTAGAAGGCCCTCTT1410.2375216885939053No Hit
CGCTGACGTACACTCTAGTATCAGGCCCCCTCAAAGCCGAGATCGCGCAG1370.2307834846621633No Hit
CGCTGACGTACACTCTAGTATCCCGCACTCAGAATGAAGTGGATGATGGC1370.2307834846621633No Hit
CGCTGACGTACACTCTAGTATCCGGACCACTCATTTATTCCTACGATATC1360.2290989336792278No Hit
CGCTGACGTACACTCTAGTATCGGGGGGGCAAATGGAGTAAAAGGATTTT1270.2139379748328083No Hit
CGCTGACGTACACTCTAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGA1230.2071997709010663No Hit
CGCTGACGTACACTCTAGTATCGCGGGGCAAATGGAGTAAAAGGATTTTC1220.20551521991813085No Hit
CGCTGACGTACACTCTAGTATCAAAATCAGATTGAAACATGCAATCAAAG1210.20383066893519533No Hit
CGCTGACGTACACTCTAGTATCCAGCCAGCAATGTTACATTTACCCAAAT1060.17856240419116284No Hit
CGCTGACGTACACTCTAGTATCCTGCCCACTCATTTATTCCTACGATATC1030.17350875124235635No Hit
CGCTGACGTACACTCTAGTATCATGCGAACAGAGGTTATAAGAATGATGG1020.17182420025942086No Hit
CGCTGACGTACACTCTAGTATCAGTAGCCCTGCAGTGGGACACTTCTGCT1020.17182420025942086No Hit
CGCTGACGTACACTCTAGTATCATGCACGTGTTATCGCATTTGTGGTAAA980.16508599632767887No Hit
CGCTGACGTACACTCTAGTATCATGCCCACTTGCTACTGCAAGCCCATAC940.15834779239593685No Hit
CGCTGACGTACACTCTAGTATCCATAGCCTTTGCCGTAGTGCTAGCCAGC890.14992503748125938No Hit
CGCTGACGTACACTCTAGTATCACCCCCCTTCAATGAAGCCGGCAATGGC890.14992503748125938No Hit
CGCTGACGTACACTCTAGTATCCCTCCCAGCGACTGAACCCCATGCACCA880.1482404864983239No Hit
CGCTGACGTACACTCTAGTATCAGTCGAAATGAATGCCCCTAATTATTAC880.1482404864983239No Hit
CGCTGACGTACACTCTAGTATCGAGGAGCAAATGGAGTAAAAGGATTTTC870.14655593551538837No Hit
CGCTGACGTACACTCTAGTATCCGGAGCAGAAATGAAAAGTGGCGAGAGC870.14655593551538837No Hit
CGCTGACGTACACTCTAGTATCAGGGAGCAAATGGAGTAAAAGGATTTTC850.14318683354951736No Hit
CGCTGACGTACACTCTAGTATCAACATCCACAGCACTCTGCTGTTCCTGT850.14318683354951736No Hit
CGCTGACGTACACTCTAGTATCTATGTTGACAAAATGACCATCGTCAACA840.14150228256658187No Hit
CGCTGACGTACACTCTAGTATCCCGCTATATCCTGACCACTCATTTATTC820.1381331806007109No Hit
CGCTGACGTACACTCTAGTATCTCGGCTTTGAGGGGGCCTGACGGGATGA810.13644862961777537No Hit
CGCTGACGTACACTCTAGTATCGCGGAGCAAATGGAGTAAAAGGATTTTC800.13476407863483988No Hit
CGCTGACGTACACTCTAGTATCGGGGGGAAAATGGACGAAGGACAAGGGT800.13476407863483988No Hit
CGCTGACGTACACTCTAGTATCCGAAGAAATAAGACCCTTCATTACTCAT800.13476407863483988No Hit
CGCTGACGTACACTCTAGTATCCGACGATATCTTGCTTTATTGAGAAGTT770.1297104256860334No Hit
CGCTGACGTACACTCTAGTATCCTGCCACTCATTTATTCCTACGATATCT760.12802587470309787No Hit
CGCTGACGTACACTCTAGTATCGAGGGGCAAATGGAGTAAAAGGATTTTC740.12465677273722689No Hit
CGCTGACGTACACTCTAGTATCCTACGCTATCTTGCTTTATTGAGAAGTT730.1229722217542914No Hit
CGCTGACGTACACTCTAGTATCGGGGCAACATCTTCTCCTATTTCATCAA720.12128767077135588No Hit
CGCTGACGTACACTCTAGTATCCTCTCGTCATTGCAGCAAATATCATTGG720.12128767077135588No Hit
CGCTGACGTACACTCTAGTATCGCGGGAGCAAATGGAGTAAAAGGATTTT710.11960311978842039No Hit
CGCTGACGTACACTCTAGTATCCAACCGGTCTCCTATATGAACTGCTAGG700.11791856880548489No Hit
CGCTGACGTACACTCTAGTATCAAGGTCTCTAATCGGTTGAAGATTACAC680.1145494668396139No Hit
CGCTGACGTACACTCTAGTATCGGAGGAGCAAATGGAGTAAAAGGATTTT670.1128649158566784No Hit
CGCTGACGTACACTCTAGTATCCAACACCATGTCAAGCTTTCAGGTAGAC630.10612671192493642No Hit
CGCTGACGTACACTCTAGTATCCTGAGCATGGCACCCATAATGTTCTCAA620.1044421609420009No Hit
CGCTGACGTACACTCTAGTATCCACCCCCCTTCAATGAAGCCGGCAATGG620.1044421609420009No Hit
CGCTGACGTACACTCTAGTATCCAGCATCGTTTGTTTTGCTCCAGAGGGT620.1044421609420009No Hit
CGCTGACGTACACTCTAGTATCGGCGGAGCAAATGGAGTAAAAGGATTTT610.10275760995906542No Hit
CGCTGACGTACACTCTAGTATCATTACCAACACTACGTCCCCTTGCCCAA610.10275760995906542No Hit
CGCTGACGTACACTCTAGTATCCCGTTATGCTTGTCTTCTAGAAGGTTAA600.1010730589761299No Hit
CGCTGACGTACACTCTAGTATCCGGAGAAATAAGACCCTTCATTACTCAT600.1010730589761299No Hit
CGCTGACGTACACTCTAGTATCATTGGGATCTTGCACCTGATATTGTGGA600.1010730589761299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAA156.478184E-495.397641
CGCTGAC57550.095.2318651
ATGACGT156.587936E-494.995793
ACGTTCA156.587936E-494.995796
GACGTTC156.587936E-494.995795
TACGTAC156.587936E-494.995795
GCTGAAG156.587936E-494.995792
TGACGTT156.587936E-494.995794
TTACGTA156.587936E-494.995794
TGACGGA156.587936E-494.995794
GCTTACG156.587936E-494.995792
TATACTC254.567719E-794.995789
ACGTATA254.567719E-794.995786
GTATACT254.567719E-794.995788
GACGTAT254.567719E-794.995785
CGTATAC254.567719E-794.995787
TGACGTA58100.094.914034
CTGACGT58050.094.913973
CGTACAC58050.094.913977
ACGTACA58000.094.9138956
Produced by FastQC (version 0.11.5)