FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC154CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC154CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278541
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT36101.2960390032347124No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTAGTTTT30371.0903242251589533No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGCAAAAGCAGGTAGATATT26540.9528220262008107No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGTAGTTTTTTACTCTA25840.9276910759995834No Hit
CGTCACGTCGTGTGCGTGCTACGGCTGGTCCATACACACAGGCAGGCAGG17320.6218115106932193No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTATTTT16590.5956035197690824No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGCAGGTAGATATTGAAAGATGAGT14750.5295450220972855No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTCGTTTT14620.5248778456313433No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGGTGTTTTTCCTTATA13780.4947207053898708No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGTCGTTTTTAAATTAT10550.3787593208899228No Hit
CGTCACGTCGTGTGCGTGCTACAATCCCAATTATACTTGCGGCAACAACA10310.370142995106645No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTACTTTT9990.35865456072894114No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGGTATTTTTCATTAAT9820.35255132996578603No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGCAAAAGCAGGGTGGCAAA9540.34249894988529517No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGTACTTTTTTGGACAG9480.3403448684394757No Hit
CGTCACGTCGTGTGCGTGCTACGGGGGGAGAATGGAAGAAAAACAAGGAT8520.30587956530636423No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGCAAAAGCAGGAGTTTAAA8490.3048025245834545No Hit
CGTCACGTCGTGTGCGTGCTACAGCAAAAGCAGGTAGATATTGAAAGATG7870.28254368297665333No Hit
CGTCACGTCGTGTGCGTGCTACAACATCCTGTTTCACTGAGAAATCACTG7810.2803896015308339No Hit
CGTCACGTCGTGTGCGTGCTACATCCAAATGTGCACCGAGCTTAAGCTCA7690.27608143863919493No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGAGTTTTT7640.27428637076767876No Hit
CGTCACGTCGTGTGCGTGCTACAAGGGTGTTTTTTATCACTAAATAAGCT7200.25848977349833596No Hit
CGTCACGTCGTGTGCGTGCTACGGGGGGGCAAAGACATAATGGATTCCCA7170.25741273277542626No Hit
CGTCACGTCGTGTGCGTGCTACAAGGAGGGCCATTCGGAAGTCTAATTGG6770.24305218980329646No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGCAAAAGCAGGTACTGATT6690.24018008120887052No Hit
CGTCACGTCGTGTGCGTGCTACAACACAGAAACGAAAACGTTCTAATCTC6460.23192276899989586No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGGGGAAGGTTACTAAATCAATAGA6170.2215113753451018No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGCAAAAGCAGGTCAATTAT6070.21792123960206936No Hit
CGTCACGTCGTGTGCGTGCTACCAAAGAATCATCACATATGAAAACAGCA5950.21361307671043042No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGCAGGAGTTTAAAATGAACCCAAA5780.20750984594727528No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGCAAAAGCAGGGTAGATAA5780.20750984594727528No Hit
CGTCACGTCGTGTGCGTGCTACGGGGGGCAAAGACATAATGGATTCCCAC5690.20427872377854606No Hit
CGTCACGTCGTGTGCGTGCTACAGGGGGGCAAAGACATAATGGATTCCCA5610.20140661518412012No Hit
CGTCACGTCGTGTGCGTGCTACAGAAACGAAAACGTTCTAATCTCTTGTT5520.19817549301539092No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGCAGGGTGGCAAAGACATAATGGA5300.19027719438071952No Hit
CGTCACGTCGTGTGCGTGCTACAACACTGAAACGAAAACGTTCTAATCTC5110.1834559364689579No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGCAGGGTAGATAATCACTCACTGA4910.17627566498289302No Hit
CGTCACGTCGTGTGCGTGCTACTGAAACGAAAACGTTCTAATCTCTTGTT4820.1730445428141638No Hit
CGTCACGTCGTGTGCGTGCTACAACAAGAGGATCACTTGAATCGTTGCAT4790.17196750209125408No Hit
CGTCACGTCGTGTGCGTGCTACGGGGGGCGAAGACATAATGGATTCCCAC4770.17124947494264758No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGCAAAAGCAGGGGAAAATA4700.16873637992252488No Hit
CGTCACGTCGTGTGCGTGCTACAGGAGCTGAGAAACCAAAGTTTCTGCCA4670.16765933919961512No Hit
CGTCACGTCGTGTGCGTGCTACAAGGGGGGAAGTTCACATATACTATCTG4480.16083808128785348No Hit
CGTCACGTCGTGTGCGTGCTACGAAAGGGGAAGGTTACTAAATCAATAGA4390.15760695911912428No Hit
CGTCACGTCGTGTGCGTGCTACATCTGCAGTGGAGTGTTCGGTGACAATC4080.14647753831572372No Hit
CGTCACGTCGTGTGCGTGCTACGAAAACGTTCTAATCTCTTGTTCCACTT4040.14504148401851075No Hit
CGTCACGTCGTGTGCGTGCTACGAGAGCTCAAAGACTCCCCGCCCCTGGA4020.14432345686990425No Hit
CGTCACGTCGTGTGCGTGCTACAAGGTAGTTTTTTACTCTAGCTCTATGC3930.14109233470117505No Hit
CGTCACGTCGTGTGCGTGCTACATGAGAACAGAATGGTTCTGGCCAGCAC3920.1407333211268718No Hit
CGTCACGTCGTGTGCGTGCTACATCCCCTTGCCCAATTAGCACATTAGCT3870.13893825325535558No Hit
CGTCACGTCGTGTGCGTGCTACAGGGGGCAAAGACATAATGGATTCCCAC3740.1342710767894134No Hit
CGTCACGTCGTGTGCGTGCTACAGGCAAATGGTAACAACAACCAATCCAT3710.13319403606650368No Hit
CGTCACGTCGTGTGCGTGCTACAAGGGTATTTTTCATTAATTGTCGTACT3680.13211699534359395No Hit
CGTCACGTCGTGTGCGTGCTACATCAACAGTCACTGGATTGCAGCTGCCC3630.13032192747207771No Hit
CGTCACGTCGTGTGCGTGCTACAACTATTGCTTCGACTATTGATTGTTCG3610.12960390032347124No Hit
CGTCACGTCGTGTGCGTGCTACAACACGAAAACGTTCTAATCTCTTGTTC3530.12673179172904528No Hit
CGTCACGTCGTGTGCGTGCTACCCTGACCAATCAGTTATTGCTACAACAT3460.12421869670892258No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGCAGGTCAATTATATTCAATATGG3350.12026954739158688No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGCAGGTACTGATTCAAAATGGAAG3260.11703842522285768No Hit
CGTCACGTCGTGTGCGTGCTACCGGCGAAGAAATGGCCACAAAGGCCGAT3240.1163203980742512No Hit
CGTCACGTCGTGTGCGTGCTACAGCAAAAGCAGGGTGGCAAAGACATAAT3240.1163203980742512No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGAGTTTTTTTCAACGA3190.11452533020273496No Hit
CGTCACGTCGTGTGCGTGCTACATCCGAATGTGCACCGAGCTTAAGCTCA3150.11308927590552198No Hit
CGTCACGTCGTGTGCGTGCTACAGCAAAAGCAGGAGTTTAAAATGAACCC3120.11201223518261226No Hit
CGTCACGTCGTGTGCGTGCTACAAGAGAGTAGATGGAAAGTGGGTGAGGG3120.11201223518261226No Hit
CGTCACGTCGTGTGCGTGCTACGGCTGGGCCATACACACAGGCAGGCAGG3010.10806308586527656No Hit
CGTCACGTCGTGTGCGTGCTACAGGAGAAAAACCCATCACTTAGGATGAA2990.10734505871667008No Hit
CGTCACGTCGTGTGCGTGCTACATGAGCTTTCTTTGGGGAATATTTCGAA2940.10554999084515385No Hit
CGTCACGTCGTGTGCGTGCTACAAGAGGATCACTTGAATCGTTGCATCTG2920.10483196369654736No Hit
CGTCACGTCGTGTGCGTGCTACAAACCATTCTTCCCCGTCAGCCATAGCA2900.10411393654794088No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGGTGTTTTTTATCACT2880.10339590939933438No Hit
CGTCACGTCGTGTGCGTGCTACAAGGAGGGAAGTTCACATATACTATCTG2820.10124182795351493No Hit
CGTCACGTCGTGTGCGTGCTACTCCAGATCTTCTAGGGAGAGTTGAACCT2790.10016478723060519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG409.094947E-1295.2843551
CGGCACG254.5300294E-795.2843551
CGTTACG353.2196112E-1095.2843551
GGTGTGC500.094.993549
GGTCGTG500.094.993546
CAAGTCG301.2290911E-894.993544
ACGTTGT254.6132845E-794.993545
GGCACGT254.6132845E-794.993542
CGTTGTG254.6132845E-794.993546
GACGTCG550.094.993544
GGCGTGT301.2290911E-894.993547
TCGGGTG353.2923708E-1094.993548
TGACGTC550.094.993543
ATGTCGT156.614404E-494.993545
GTTACGT353.2923708E-1094.993542
TCGTGAG254.6132845E-794.993548
TCATGTG353.2923708E-1094.993548
GTCGTGA254.6132845E-794.993547
ACGTCGG353.2923708E-1094.993545
ACGTCAT353.2923708E-1094.993545
Produced by FastQC (version 0.11.5)