FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC152CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC152CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50753
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAGAGATATAGACGTAGAATGAGAAAATGTGAATGTGTGTCTTCTC5601.103383051248202No Hit
CGCGTAGAGATATAGACGTAGACAGAGCAGTCAAACTTTATCGAAAGCTT3370.6640001576261502No Hit
CGCGTAGAGATATAGACGTAGAAGGCCCTCTTTTTAAACCGTGTTTAAAG2770.5457805449924142No Hit
CGCGTAGAGATATAGACGTAGATTTTTGGATAGTGAATAGTACTTGCCAC1910.37633243355072604No Hit
CGCGTAGAGATATAGACGTAGAAAAAGCAATGAAAGAGTATGGAGAGGAC1900.3743621066734971No Hit
CGCGTAGAGATATAGACGTAGAAACAGCTACTTGTGTTGGAAAGCAAATA1310.2581128209169901No Hit
CGCGTAGAGATATAGACGTAGATATTGAAAGATGAGTCTTCTAACCGAGG1120.22067661024964044No Hit
CGCGTAGAGATATAGACGTAGAACAAGCAGCTGAGGCCATGGAGGTTGCT1110.2187062833724115No Hit
CGCGTAGAGATATAGACGTAGAAGGACCTCTTTTTAAACCGTGTTTAAAG1060.20885464898626685No Hit
CGCGTAGAGATATAGACGTAGAAGGCGCTCTTTTTAAACCGTGTTTAAAG990.1950623608456643No Hit
CGCGTAGAGATATAGACGTAGAAGCAGCTGAGGCCATGGAGGTTGCTAGT980.19309203396843536No Hit
CGCGTAGAGATATAGACGTAGAGAGGGGTTTGAGATGATTTGGGATCCTA940.18521072645951964No Hit
CGCGTAGAGATATAGACGTAGAAGGACCAAAAGTAACAGACTTAGAAAGG920.18127007270506176No Hit
CGCGTAGAGATATAGACGTAGAAAAAGCAGGTAGATATTGAAAGATGAGT870.1714184383189171No Hit
CGCGTAGAGATATAGACGTAGAAACAAAGTAGTTTTTTACTCTAGCTCTA810.1595964770555435No Hit
CGCGTAGAGATATAGACGTAGAAACAAGGGTGTTTTTTATCACTAAATAA790.15565582330108566No Hit
CGCGTAGAGATATAGACGTAGAAGGGGGTTTGAGATGATTTGGGATCCTA760.14974484266939886No Hit
CGCGTAGAGATATAGACGTAGAAGGCAATGGTGAAATTTCGCCAACAATT760.14974484266939886No Hit
CGCGTAGAGATATAGACGTAGAGGGGGGCGAAGACATAATGGATTCCCAC740.14580418891494099No Hit
CGCGTAGAGATATAGACGTAGAACCCAAATCAAAAGATAATAACCATTGG740.14580418891494099No Hit
CGCGTAGAGATATAGACGTAGATGAGTCTTCTAACCGAGGTCGAAACGTA720.14186353516048314No Hit
CGCGTAGAGATATAGACGTAGACCCAGAAGCAAGGTCTTATACAGTCCAA720.14186353516048314No Hit
CGCGTAGAGATATAGACGTAGAAAGGGGTTTGAGATGATTTGGGATCCTA720.14186353516048314No Hit
CGCGTAGAGATATAGACGTAGAAGGGGTTTGAGATGATTTGGGATCCTAA700.13792288140602524No Hit
CGCGTAGAGATATAGACGTAGAAGGGGGGCAAAGACATAATGGATTCCCA690.13595255452879634No Hit
CGCGTAGAGATATAGACGTAGACGGCCCTCTTTTTAAACCGTGTTTAAAG610.12018993951096488No Hit
CGCGTAGAGATATAGACGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTA590.11624928575650699No Hit
CGCGTAGAGATATAGACGTAGAATGCCCTCTTTTTAAACCGTGTTTAAAG580.11427895887927808No Hit
CGCGTAGAGATATAGACGTAGAAGCCCCTCTTTTTAAACCGTGTTTAAAG570.11230863200204913No Hit
CGCGTAGAGATATAGACGTAGAAATAGCACTCAGTTATTCTGCTGGTGCA570.11230863200204913No Hit
CGCGTAGAGATATAGACGTAGAAGGCCAATTATATTCAATATGGAAAGAA570.11230863200204913No Hit
CGCGTAGAGATATAGACGTAGATATTGCAAGATGAGTCTTCTAACCGAGG560.11033830512482022No Hit
CGCGTAGAGATATAGACGTAGATATTGGAAGATGAGTCTTCTAACCGAGG560.11033830512482022No Hit
CGCGTAGAGATATAGACGTAGAACGCCACAAAAAGAAATGCTGCTCCCAC560.11033830512482022No Hit
CGCGTAGAGATATAGACGTAGAGGGGGGCAAAGACATAATGGATTCCCAC530.10442732449313342No Hit
CGCGTAGAGATATAGACGTAGAAGGGGGCAAAGACATAATGGATTCCCAC530.10442732449313342No Hit
CGCGTAGAGATATAGACGTAGAAGGCACTCTTTTTAAACCGTGTTTAAAG520.10245699761590447No Hit
CGCGTAGAGATATAGACGTAGACAGGGGGCAAAGACATAATGGATTCCCA520.10245699761590447No Hit
CGCGTAGAGATATAGACGTAGACCCCCAAGATCAAAGGTCCCAGGTTCCA510.10048667073867554No Hit
CGCGTAGAGATATAGACGTAGATGTTGAAAGATGAGTCTTCTAACCGAGG510.10048667073867554No Hit
CGCGTAGAGATATAGACGTAGAAGGGGGCGAAGACATAATGGATTCCCAC510.10048667073867554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAG254.5304296E-795.0927051
CGCGGAG201.719381E-595.0927051
GGATATA156.581491E-494.9998
GCGTGGA156.581491E-494.9992
CGTGGAG156.581491E-494.9993
CGTAAAG156.581491E-494.9993
GCGTAGG156.581491E-494.9992
GCGTAAA156.581491E-494.9992
TAGGGAT156.581491E-494.9995
GATATAG49500.094.615189
GCGTAGA49600.094.5201952
CGCGTAG49550.094.516971
GAGATAT49550.094.423847
AGATATA49600.094.328678
TAGAGAT49850.094.0461655
AGAGATA50000.093.859036
GTAGAGA55300.085.0352864
CGCGTGG200.002054660671.319531
CGCGTAA200.002054660671.319531
CGTAGGG200.002062721471.249273
Produced by FastQC (version 0.11.5)