FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC149CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC149CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625116
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATAGACGACTAGTACGTGAATGAGAAAATGTGAATGTGTGTCTTCTC55030.8803166132365833No Hit
CGTATAGACGACTAGTACGTGAAGGACCAAAAGTAACAGACTTAGAAAGG29530.47239232398466846No Hit
CGTATAGACGACTAGTACGTGACGCCACAAAAAGAAATGCTGCTCCCACT22260.3560939089704951No Hit
CGTATAGACGACTAGTACGTGAGGGGCGGGAACAACTAGGTCAGAAGTTT22180.3548141464944107No Hit
CGTATAGACGACTAGTACGTGACAGAGCAGTCAAACTTTATCGAAAGCTT21110.33769732337678127No Hit
CGTATAGACGACTAGTACGTGAACCCAAATCAAAAGATAATAACCATTGG16330.2612315154307361No Hit
CGTATAGACGACTAGTACGTGAATGAGCAAATGTGAATGTGTGTCTTCTC16090.25739222800248274No Hit
CGTATAGACGACTAGTACGTGAACAAGCAGCTGAGGCCATGGAGGTTGCT15280.2444346329321278No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGTAGTTTTTTACTC14670.23467644405198396No Hit
CGTATAGACGACTAGTACGTGACCCAGAAGCAAGGTCTTATACAGTCCAA14000.22395843331477677No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGGTGTTTTTTATCA13450.21516006629169626No Hit
CGTATAGACGACTAGTACGTGAAGGCCCTCTTTTTAAACCGTGTTTAAAG13190.2110008382444218No Hit
CGTATAGACGACTAGTACGTGAACGCCACAAAAAGAAATGCTGCTCCCAC12300.19676348069798244No Hit
CGTATAGACGACTAGTACGTGAGGGGCCGCAAGTATAATTGGGATTGTGC12200.1951637776028769No Hit
CGTATAGACGACTAGTACGTGAATCAGCAAAAGCAGGTAGATATTGAAAG11940.19100454955560248No Hit
CGTATAGACGACTAGTACGTGAAAACCCTTTTACTCCGTTTGCTCCATCA11880.19004472769853914No Hit
CGTATAGACGACTAGTACGTGAAGGGCGGGAACAACTAGGTCAGAAGTTT11850.1895648167700075No Hit
CGTATAGACGACTAGTACGTGAAGGGGGGGAACAACTAGGTCAGAAGTTT11010.17612731077112087No Hit
CGTATAGACGACTAGTACGTGACTTAGAAAGGGGTTTGAGATGATTTGGG10610.16972849839069867No Hit
CGTATAGACGACTAGTACGTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG9540.15261167527306932No Hit
CGTATAGACGACTAGTACGTGACAGCCCATATTGAAATAATATTTCCTAT9220.14749262536873156No Hit
CGTATAGACGACTAGTACGTGACTTCCAGTTTGGATTGAGTGACTAGCCC8510.13613473339348217No Hit
CGTATAGACGACTAGTACGTGAATGTGTGTCTTCTCAGATTTAATTTTGT8440.1350149412269083No Hit
CGTATAGACGACTAGTACGTGAACCCGAATCAAAAGATAATAACCATTGG8240.13181553503669718No Hit
CGTATAGACGACTAGTACGTGAACACAAATCCTAAAATCCCCTTAGTCAG8080.1292560100845283No Hit
CGTATAGACGACTAGTACGTGACGCGTGATCAGTAGAAACAAGGTAGTTT8070.12909603977501774No Hit
CGTATAGACGACTAGTACGTGAAAGGGGTTTGAGATGATTTGGGATCCTA7850.12557669296578555No Hit
CGTATAGACGACTAGTACGTGAAACAGGATGTTGGACGAAACTTCCGCTG7700.12317713832312722No Hit
CGTATAGACGACTAGTACGTGACTGCCCATATTGAAATAATATTTCCTAT7510.12013770244242669No Hit
CGTATAGACGACTAGTACGTGATTGTGCACTTGATATTGTGGATTATTGA7360.11773814779976836No Hit
CGTATAGACGACTAGTACGTGACGCGTGATCAGTAGAAACAAGGGTGTTT7280.11645838532368394No Hit
CGTATAGACGACTAGTACGTGAGGGGGGGGAACAACTAGGTCAGAAGTTT7100.11357891975249394No Hit
CGTATAGACGACTAGTACGTGACCGCCATTTTCCGTTTCTGTGTTGTAGT6630.10606031520549787No Hit
CGTATAGACGACTAGTACGTGATCAGCAAAAGCAGGTAGATATTGAAAGA6390.10222102777724454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA1500.095.003928
TAGACGG1500.094.996325
CGTATAG620500.093.737311
ACGACTA615250.093.606458
CGACTAG616800.093.586879
GACGACT616200.093.5854957
ATAGACG618500.093.552564
GTATAGA621200.093.53592
TAGACGA617700.093.5122455
TATAGAC619850.093.502073
CGTGTAG1450.091.889631
ATAGCCG1450.091.720584
CGTATGG2050.090.52891
AGACGAC638100.090.232366
CGTTTAG800.089.22321
GACGCCT1600.089.066187
CGATTAG700.088.217929
CTACTAG1150.086.742719
TAGCCGA1600.086.0904165
ACGTCTA500.085.5035258
Produced by FastQC (version 0.11.5)