FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC140CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC140CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238833
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCGCGTCACGTATGACTGATCAGCAAAAGCAGGTAGATATTGAAAG29731.2448028538769769No Hit
CGTCGCGCGTCACGTATGACTGCTCTGTCCATATTATTTGGATCCCCATT28301.1849283809188849No Hit
CGTCGCGCGTCACGTATGACTGAAACGAAAACGTTCTAATCTCTTGTTCC22130.9265888717220819No Hit
CGTCGCGCGTCACGTATGACTGATTCGAACTTTGAATTGTATGGGGACGG21600.9043976334928591No Hit
CGTCGCGCGTCACGTATGACTGAGTGCTATTTCTTTGGCCCCATGGAATG18160.7603639363069592No Hit
CGTCGCGCGTCACGTATGACTGAGGACCCAAATGCACTTTTAAAGCACAG14950.625960399107326No Hit
CGTCGCGCGTCACGTATGACTGATCAGCAAAAGCAGGCAGATATTGAAAG14510.6075374843509901No Hit
CGTCGCGCGTCACGTATGACTGAGAACAGAATGGTTCTGGCCAGCACTAC11650.48778853843480585No Hit
CGTCGCGCGTCACGTATGACTGATTCAAAATGGAAGATTTTGTACGACAA11500.48150799931332766No Hit
CGTCGCGCGTCACGTATGACTGGATTGCAGCTGCCCTCTCCATCTTCGGG11290.47271524454325825No Hit
CGTCGCGCGTCACGTATGACTGAGGAGATTTTAGGATTTGTGTTCACGCT9470.39651136986932295No Hit
CGTCGCGCGTCACGTATGACTGGGGGGATTTTAGGATTTGTGTTCACGCT9320.39023083074784476No Hit
CGTCGCGCGTCACGTATGACTGAACCCAAATCAAAAGATAATAACCATTG8470.35464110905946833No Hit
CGTCGCGCGTCACGTATGACTGCGGCGCCTGCTGCTCCAGATCTTCTAGG7600.3182139821548948No Hit
CGTCGCGCGTCACGTATGACTGACGAAAGGAGTCCCAAAGACCTCTGCTT6200.2595956170210984No Hit
CGTCGCGCGTCACGTATGACTGAGGATCCAAATGCACTTTTAAAGCACAG6180.25875821180490133No Hit
CGTCGCGCGTCACGTATGACTGGGTGTACGTTCTGGAAAGGAAGACTGCT4870.20390817014399182No Hit
CGTCGCGCGTCACGTATGACTGGCAACCTCCATGGCCTCAGCTGCTTGTT4700.19679022580631655No Hit
CGTCGCGCGTCACGTATGACTGAGGGGATTTTAGGATTTGTGTTCACGCT4270.17878601365807908No Hit
CGTCGCGCGTCACGTATGACTGGTTTGAAGCAGTTTGAAAGGGTCTACTC4080.1708306641042067No Hit
CGTCGCGCGTCACGTATGACTGGGGGCATTTTGGACAAAGCGTCTACGCT4050.16957455627991108No Hit
CGTCGCGCGTCACGTATGACTGAAAAGGGGAAGGTTACTAAATCAATAGA3960.16580623280702417No Hit
CGTCGCGCGTCACGTATGACTGAGGAGCCAAATGCACTTTTAAAGCACAG3930.1645501249827285No Hit
CGTCGCGCGTCACGTATGACTGCAGGGGGCAAAGACATAATGGATTCCCA3920.16413142237462996No Hit
CGTCGCGCGTCACGTATGACTGATGATCCACTTGTTCTCTTAAATGTGAA3700.15491996499646196No Hit
CGTCGCGCGTCACGTATGACTGACTCAGGGCCATTAATCTCCCACATCAT3560.1490581284830823No Hit
CGTCGCGCGTCACGTATGACTGAGGCCATGGAGGTTGCTAGTCAGGCCAG3450.14445239979399832No Hit
CGTCGCGCGTCACGTATGACTGCTTTTACCATGCAATCTTCTTGTGAGAA3440.14403369718589978No Hit
CGTCGCGCGTCACGTATGACTGTTGAGTAGATCGCCAGAATCTGATAGAC3410.14277758936160412No Hit
CGTCGCGCGTCACGTATGACTGCACCCCCATTCGTTTCTGATAGGCCTGT3230.13524094241583032No Hit
CGTCGCGCGTCACGTATGACTGATCAGCAAAAGCAGGGTGGCAAAGACAT3210.13440353719963322No Hit
CGTCGCGCGTCACGTATGACTGAGAAGAAATAAGGCGGATTTGGCGCCAA3170.132728726767239No Hit
CGTCGCGCGTCACGTATGACTGAGTCCCAATGGCTCTCATTGCCTGCACC3120.1306352137267463No Hit
CGTCGCGCGTCACGTATGACTGAACAGAATGGTTCTGGCCAGCACTACAG3000.12561078242956375No Hit
CGTCGCGCGTCACGTATGACTGAGGAGAAAAAAGAAGAACTCCAGAATTG2980.12477337721336666No Hit
CGTCGCGCGTCACGTATGACTGCCACAAAAAGAAATGCTGCTCCCACTAG2970.12435467460526811No Hit
CGTCGCGCGTCACGTATGACTGAAAATTGGGTGCATTCAACTCTATTGAT2940.12309856678097249No Hit
CGTCGCGCGTCACGTATGACTGATTCGAAATGGAAGATTTTGTACGACAA2910.12184245895667684No Hit
CGTCGCGCGTCACGTATGACTGGCAAGTGCACCAGCAGAATAACTGAGTG2880.1205863511323812No Hit
CGTCGCGCGTCACGTATGACTGTCGGTATTTGTCCATCCATTAGGATCCC2800.11723673026759282No Hit
CGTCGCGCGTCACGTATGACTGGTAGATATTGAAAGATGAGTCTTCTAAC2730.11430581201090301No Hit
CGTCGCGCGTCACGTATGACTGAGGGGGCAAAGACATAATGGATTCCCAC2720.11388710940280447No Hit
CGTCGCGCGTCACGTATGACTGAACAGCAAAAGCAGGTAGATATTGAAAG2690.11263100157850882No Hit
CGTCGCGCGTCACGTATGACTGATTAACTCCAATACCATTGTCCCGACTC2680.11221229897041028No Hit
CGTCGCGCGTCACGTATGACTGGAAAGGGGAAGGTTACTAAATCAATAGA2670.11179359636231173No Hit
CGTCGCGCGTCACGTATGACTGAAACAAGGTAGTTTTTTACTCTAGCTCT2660.1113748937542132No Hit
CGTCGCGCGTCACGTATGACTGAGGGCATTTTGGACAAAGCGTCTACGCT2650.11095619114611466No Hit
CGTCGCGCGTCACGTATGACTGAGTCCCGTTTTCGTTTCATTACCAACAC2610.10928138071372047No Hit
CGTCGCGCGTCACGTATGACTGGGGGGGGGAACTCGTCCTTTATGACAAA2480.10383824680843938No Hit
CGTCGCGCGTCACGTATGACTGAGAACAGAATGGTTCTGGCCTGACTAGC2440.10216343637604519No Hit
CGTCGCGCGTCACGTATGACTGATGTTCTTCCCTCTGTATTCCCAGAGAA2440.10216343637604519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG254.547619E-795.2153241
CGGCGCG450.095.2153241
GGTCGCG500.095.2153241
CGTTGCG353.237801E-1095.2153241
CGTAGCG156.552205E-495.2153241
CGTCGAG254.547619E-795.2153241
AGTCGCG201.7215209E-595.215321
GCGCGTG301.22818165E-894.9960255
GGCACGT700.094.9960259
GCGCGCG450.094.9960253
GCGCGCC156.6125527E-494.9960255
TAGCGCG156.6125527E-494.9960253
CGCGCGC156.6125527E-494.9960254
CGCGCCA156.6125527E-494.9960256
GGTCACG301.22818165E-894.9960258
GTAGCGC156.6125527E-494.9960252
CGCTCGT156.6125527E-494.9960254
TGCGCGT353.2923708E-1094.9960254
GAGCGTC254.610538E-794.9960255
GTCACGG409.094947E-1294.9960259
Produced by FastQC (version 0.11.5)