FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC139CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC139CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149773
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTAGATACGACAGAGAGAGGGGGCCTGACGGGATGATAGAGAGAAC9700.6476467721151342No Hit
CGTAGTAGATACGACAGAGAGATGAGTCTTCTAACCGAGGTCGAAACGTA9420.6289518137448005No Hit
CGTAGTAGATACGACAGAGAGAAGTAGAAACAAGGTAGTTTTTTACTCTA7640.51010529267625No Hit
CGTAGTAGATACGACAGAGAGACGCCACAAAAAGAAATGCTGCTCCCACT6220.4152951466552717No Hit
CGTAGTAGATACGACAGAGAGAAGGCGATCAATAATCCACAATATCAAGT5520.3685577507294372No Hit
CGTAGTAGATACGACAGAGAGAGGGGTTTGAGATGATTTGGGATCCTAAT4970.33183551107342446No Hit
CGTAGTAGATACGACAGAGAGACAGAGCAGTCAAACTTTATCGAAAGCTT4930.32916480273480536No Hit
CGTAGTAGATACGACAGAGAGAATGAGAAAATGTGAATGTGTGTCTTCTC4540.303125396433269No Hit
CGTAGTAGATACGACAGAGAGAAGGCCCTCTTTTTAAACCGTGTTTAAAG4220.2817597297243161No Hit
CGTAGTAGATACGACAGAGAGAAACAGCTACTTGTGTTGGAAAGCAAATA3370.22500717752866004No Hit
CGTAGTAGATACGACAGAGAGAGGGAGATAACATTCCATGGGGCCAAAGA3130.2089829274969454No Hit
CGTAGTAGATACGACAGAGAGAGGGGGATAACATTCCATGGGGCCAAAGA3110.20764757332763584No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCTGTGACCACCGAATCAGCATTT3100.20697989624298108No Hit
CGTAGTAGATACGACAGAGAGAAAAAGCAATGAAAGAGTATGGAGAGGAC3000.20030312539643327No Hit
CGTAGTAGATACGACAGAGAGAAGGGGTTTGAGATGATTTGGGATCCTAA2940.19629706288850463No Hit
CGTAGTAGATACGACAGAGAGAAGGCGCTCTTTTTAAACCGTGTTTAAAG2890.1929586774652307No Hit
CGTAGTAGATACGACAGAGAGAAGTAGAAACAAGGCAGTTTTTTACTCTA2870.19162332329592116No Hit
CGTAGTAGATACGACAGAGAGAAGCAAAAGCAGGTAGATATTGAAAGATG2760.1842788753647186No Hit
CGTAGTAGATACGACAGAGAGACGCGTGATCAGTAGAAACAAGGTAGTTT2620.17493139617955172No Hit
CGTAGTAGATACGACAGAGAGAACGCCACAAAAAGAAATGCTGCTCCCAC2540.1695899795023135No Hit
CGTAGTAGATACGACAGAGAGAGGGGGTGCAGATGCAACGATTCAAGTGA2500.1669192711636944No Hit
CGTAGTAGATACGACAGAGAGAACAAGCAGATGAGTCCCAATGGCTCTCA2250.15022734404732496No Hit
CGTAGTAGATACGACAGAGAGAACCCAAATCAAAAGATAATAACCATTGG2240.14955966696267017No Hit
CGTAGTAGATACGACAGAGAGAACAAGCAGCTGAGGCCATGGAGGTTGCT2230.1488919898780154No Hit
CGTAGTAGATACGACAGAGAGAAAAAGCAGGTAGATATTGAAAGATGAGT2190.1462212815393963No Hit
CGTAGTAGATACGACAGAGAGAAGGACCAAAAGTAACAGACTTAGAAAGG2110.14087986486215806No Hit
CGTAGTAGATACGACAGAGAGATGGGGGTGCAGATGCAACGATTCAAGTG2050.13687380235422938No Hit
CGTAGTAGATACGACAGAGAGAAGGCAATGGTGAAATTTCGCCAACAATT1950.1301970315076816No Hit
CGTAGTAGATACGACAGAGAGAGGGGGGCTGTGACCACCGAATCAGCATT1940.12952935442302685No Hit
CGTAGTAGATACGACAGAGAGAACGAGCAGCTGAGGCCATGGAGGTTGCT1830.1221849064918243No Hit
CGTAGTAGATACGACAGAGAGAGGGGGGCAAAGACATAATGGATTCCCAC1780.11884652106855041No Hit
CGTAGTAGATACGACAGAGAGAAGTCAAACTTTATCGAAAGCTTAAGAGG1740.1161758127299313No Hit
CGTAGTAGATACGACAGAGAGAAGGGGGGCAAAGACATAATGGATTCCCA1730.11550813564527651No Hit
CGTAGTAGATACGACAGAGAGAGGGGGGGCAAAGACATAATGGATTCCCA1730.11550813564527651No Hit
CGTAGTAGATACGACAGAGAGAATAACCATTGGATCAATCAGTATAGCAA1730.11550813564527651No Hit
CGTAGTAGATACGACAGAGAGAAAGGGGTTTGAGATGATTTGGGATCCTA1720.11484045856062174No Hit
CGTAGTAGATACGACAGAGAGAGAGGGGTTTGAGATGATTTGGGATCCTA1690.1128374273066574No Hit
CGTAGTAGATACGACAGAGAGACACAAAAAGAAATGCTGCTCCCACTAGT1640.10949904188338351No Hit
CGTAGTAGATACGACAGAGAGAGTGGGGGTGCAGATGCAACGATTCAAGT1590.10616065646010962No Hit
CGTAGTAGATACGACAGAGAGAAGCAAAAGCAGGCAGATATTGAAAGATG1580.10549297937545486No Hit
CGTAGTAGATACGACAGAGAGACCCAGAAGCAAGGTCTTATACAGTCCAA1580.10549297937545486No Hit
CGTAGTAGATACGACAGAGAGACTTGAAGATGTATTTGCTGGAAAGAATA1560.10415762520614531No Hit
CGTAGTAGATACGACAGAGAGAAGGGGGCAAAGACATAATGGATTCCCAC1520.1014869168675262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA550.095.215531
CGTATTA156.5473217E-495.215531
CCTAGTA156.5473217E-495.215531
CGTAGTC201.719391E-595.215531
CGCAGTA156.5473217E-495.215531
GGTAGTA201.719391E-595.215531
CGTCGTA254.539197E-795.215521
GATACGG500.094.992998
GTCGATA201.7394967E-594.992995
TCGATAC201.7394967E-594.992996
CTAGTAG156.608532E-494.992992
GTCGTAG254.6029527E-794.992992
GATGCGA254.6029527E-794.992998
TAGATAG201.7394967E-594.992996
ATGCGAC254.6029527E-794.992999
AGTAGGT409.094947E-1294.992994
ATACGGC450.094.992999
GTGGTAG550.094.992992
CGATACG201.7394967E-594.992997
GTAGTCG201.7394967E-594.992992
Produced by FastQC (version 0.11.5)