FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC136CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC136CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040532
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGAGTACGATATATACTGAAACGAAAACGTTCTAATCTCTTGTTCC152211.4628094090330714No Hit
CGTATGAGTACGATATATACTGATTCAAAATGGAAGATTTTGTACGACAA73960.7107902496030877No Hit
CGTATGAGTACGATATATACTGAAGACCCAGATGAAGGCACAGCTGGAGT24570.23612921082676938No Hit
CGTATGAGTACGATATATACTGGGGGACCTCCATTCACTACAACACAGAA21460.2062406538193924No Hit
CGTATGAGTACGATATATACTGATCAGCAAAAGCAGGTCTCCCCATGCCT19870.19096000891851475No Hit
CGTATGAGTACGATATATACTGTGGGGAGGGGCCAGGAGGCTGAGGGAGT18450.1773131436611272No Hit
CGTATGAGTACGATATATACTGCGGCAACAACAAGAGGATCACTTGAATC16720.16068703317149305No Hit
CGTATGAGTACGATATATACTGGGGGCGGGAACAACTAGGTCAGAAGTTT16170.15540127550137814No Hit
CGTATGAGTACGATATATACTGGGGGGAACAACTAGGTCAGAAGTTTGAA15610.15001941314635206No Hit
CGTATGAGTACGATATATACTGAGAACAGAATGGTTCTGGCCAGCACTAC15260.14665574917446075No Hit
CGTATGAGTACGATATATACTGATTCGAACTTTGAATTGTATGGGGACGG13090.12580103254873468No Hit
CGTATGAGTACGATATATACTGTGTGGGGAGGGGCCAGGAGGCTGAGGGA12950.12445556695997817No Hit
CGTATGAGTACGATATATACTGCAAACAGAATATGGATAAAGTGGTAGGA12000.11532562189341605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA1027700.095.0581
TACGGTA1550.095.0003369
AGTACGA1019800.094.9118357
GTACGAT1019150.094.907128
GAGTACG1021300.094.898026
TACGATA1018900.094.823189
ATGAGTA1025300.094.7502444
TGAGTAC1023900.094.703435
GTATGAG1033800.094.582272
TATGAGT1029550.094.497513
GTACGGT1750.086.8574458
GTGCGAT2600.085.8656858
GGACGAT3850.085.130178
AGTACGG1800.084.444747
GACGATA4350.080.804889
CTACGAT650.080.3848958
GTCCGAT600.079.166958
TTACGAT600.079.166958
AACGATA1800.076.5280469
GTACGAA1000.076.000278
Produced by FastQC (version 0.11.5)