FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC134CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC134CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52014
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTATATAGTCTGTAGTCGCACAGCGACTTGAAGATGTATTTGCTGG12262.357057715230515No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTAGATATTGAAAG2390.4594916753181836No Hit
CGTCGTATATAGTCTGTAGTCGAAACGCACGTTCTCTCTATCATCCCGTC2050.39412465874572233No Hit
CGTCGTATATAGTCTGTAGTCGAAACAACACTGGAATATGCAACCAAAGA1400.26915830353366405No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTACTGATTCAAAA1310.25185526973507133No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGCAGATATTGAAAG1290.24801015111316185No Hit
CGTCGTATATAGTCTGTAGTCGCAAATAGGAAATATTATTTCAATATGGG1180.2268619986926597No Hit
CGTCGTATATAGTCTGTAGTCGCCACAAAAAGAAATGCTGCTCCCACTAG1160.22301688007075018No Hit
CGTCGTATATAGTCTGTAGTCGGGGGCAGGAATGAGAATACTGGAGAATT1140.21917176144884068No Hit
CGTCGTATATAGTCTGTAGTCGATGAGCAAGGCGAATCAATAATAGTAGA830.15957242280924366No Hit
CGTCGTATATAGTCTGTAGTCGTTGTAGTTGAATACTTGTGAGGGGTAAT790.15188218556542468No Hit
CGTCGTATATAGTCTGTAGTCGAGGTCGAAACGTACGTTCTCTCTATCAT770.1480370669435152No Hit
CGTCGTATATAGTCTGTAGTCGGGGGGCTATATGAAGCAATTGAGGAGTG750.14419194832160573No Hit
CGTCGTATATAGTCTGTAGTCGAGGACCCAAATGCACTTTTAAAGCACAG730.14034682969969622No Hit
CGTCGTATATAGTCTGTAGTCGAGGGCGGGAACAACTAGGTCAGAAGTTT690.13265659245587727No Hit
CGTCGTATATAGTCTGTAGTCGAGTCCCGTTTTCGTTTCATTACCAACAC690.13265659245587727No Hit
CGTCGTATATAGTCTGTAGTCGAAACAAGGTAGTTTTTTACTCTAGCTCT690.13265659245587727No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTAAACCATTTGAA690.13265659245587727No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGCCAATTATATTCA660.126888914523013No Hit
CGTCGTATATAGTCTGTAGTCGCTCAAAACTATTCTCTGTCGTCTTCAAT620.11919867727919406No Hit
CGTCGTATATAGTCTGTAGTCGGGGGACTCGAACTGTGTTATCATTCCAT620.11919867727919406No Hit
CGTCGTATATAGTCTGTAGTCGGGGGCGGGAACAACTAGGTCAGAAGTTT570.10958588072442034No Hit
CGTCGTATATAGTCTGTAGTCGAATCAATAATAGTAGAGCCTGAGGACCC560.1076633214134656No Hit
CGTCGTATATAGTCTGTAGTCGGGGGGGCAAATGCAACAACAAGAGGATC540.10381820279155612No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTCAATTATATTCA530.10189564348060137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTA156.532423E-495.1811141
CGTATGT156.582669E-494.998084
ATCGTAT156.582669E-494.998082
CGTCGTA51650.093.522591
TCGTATA52000.093.2625353
CGTATAT52300.093.181664
GTCGTAT52050.093.172942
ATAGTCT51950.093.169439
TATAGTC51950.093.169438
ATATAGT51950.093.169437
TATATAG52450.093.0963066
GTATATA52450.093.0057455
TATATCG200.002063089371.248566
GTATATC200.002063089371.248565
ATATAGG353.371766E-667.855777
CATCGTA250.00495686357.108671
GTATGTA250.004994828356.9988445
TATGTAG250.004994828356.9988446
TATAGCC352.763477E-454.2846158
ATAGGCT451.4960415E-552.776719
Produced by FastQC (version 0.11.5)