FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC117CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC117CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149978
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTATCACTACTAGTACTCTCAAACCCCTTTCTAAGTCTGTTACTTTTG15941.0628225473069384No Hit
CGCTATCACTACTAGTACTCTCAGGCAAATGGTAACAACAAGAGGATCAC14280.9521396471482485No Hit
CGCTATCACTACTAGTACTCTCAACCCAGAAGCAAGGTCTTATACAGTCC13890.9261358332555443No Hit
CGCTATCACTACTAGTACTCTCAGGCCCCCTCAAAGCCGAGATCGCACAG9580.6387603515182227No Hit
CGCTATCACTACTAGTACTCTCAGCACCGTCTGGCCAAGACCAGTTTGCA7270.48473776153835896No Hit
CGCTATCACTACTAGTACTCTCGATATTCTTCCCTCATAGACTCTGGTAC6280.41872808011841733No Hit
CGCTATCACTACTAGTACTCTCCTACGCGTGATCAGCAAAAGCAGGTAGA6160.4107269066129699No Hit
CGCTATCACTACTAGTACTCTCAAGCCCCTTTCTAAGTCTGTTACTTTTG5500.3667204523330088No Hit
CGCTATCACTACTAGTACTCTCAGAAGCAAGGTCTTATACAGTCCAATCC5490.36605368787422154No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACATTAGTGTTGTTAATCAT5090.33938310952273No Hit
CGCTATCACTACTAGTACTCTCAGCCCAGAAGCAAGGTCTTATACAGTCC4880.32538105588819694No Hit
CGCTATCACTACTAGTACTCTCAATCCAAACTGGAAGTCAAAACAACACT4480.2987104775367054No Hit
CGCTATCACTACTAGTACTCTCTAACCGAGGTCGAAACGTACGTTCTCTC4250.2833748949845978No Hit
CGCTATCACTACTAGTACTCTCAGGCAAATGGTAACAACAACCAATCCAT4180.2787075437730867No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACATTAGTGTTGTTAATATT4140.27604048593793756No Hit
CGCTATCACTACTAGTACTCTCGGGGGACTCGAACTGTGTTATCATTCCA3650.2433690274573604No Hit
CGCTATCACTACTAGTACTCTCAACCGAGGTCGAAACGTACGTTCTCTCT3620.24136873408099857No Hit
CGCTATCACTACTAGTACTCTCAACCCCGAAGCAAGGTCTTATACAGTCC3510.23403432503433835No Hit
CGCTATCACTACTAGTACTCTCCGTAGAAGGCCCTCTTTTTAAACCGTGT3500.23336756057555108No Hit
CGCTATCACTACTAGTACTCTCAAGCCAAACTGGAAGTCAAAACAACACT3450.23003373828161464No Hit
CGCTATCACTACTAGTACTCTCAGGCAAATGGTAACAGCAACCTCCATGG3360.22403285815252905No Hit
CGCTATCACTACTAGTACTCTCCAGCAACAACATTAGTGTTGTTAATATT3220.21469815572950698No Hit
CGCTATCACTACTAGTACTCTCAGACCCCTTTCTAAGTCTGTTACTTTTG3070.20469668884769768No Hit
CGCTATCACTACTAGTACTCTCAAGCCAGAAGCAAGGTCTTATACAGTCC3010.2006961020949739No Hit
CGCTATCACTACTAGTACTCTCCTACGCGTGATCAGCAAAAGCAGGCAGA2990.19936257317739936No Hit
CGCTATCACTACTAGTACTCTCAGGCAAATGGCAACAACAAGAGGATCAC2990.19936257317739936No Hit
CGCTATCACTACTAGTACTCTCATTGCCTGCACCATCTGCCTGGCCTGAC2790.18602728400165358No Hit
CGCTATCACTACTAGTACTCTCAGGCAAATGGTAACAACAACAAGAGGAT2680.1786928749549934No Hit
CGCTATCACTACTAGTACTCTCTATCATCCCGTCAGGCCCCCTCAAAGCC2550.17002493699075863No Hit
CGCTATCACTACTAGTACTCTCAATCGAAACTGGAAGTCAAAACAACACT2540.16935817253197136No Hit
CGCTATCACTACTAGTACTCTCAGCAAAAGCAGGTAGATATTGAAAGATG2510.16735787915560948No Hit
CGCTATCACTACTAGTACTCTCAAAAGCAGGTAGATATTGAAAGATGAGT2460.16402405686167304No Hit
CGCTATCACTACTAGTACTCTCAGGCGAATGGTAACAACAACCTCCATGG2440.16269052794409847No Hit
CGCTATCACTACTAGTACTCTCAACCGAGAAGCAAGGTCTTATACAGTCC2350.15668964781501288No Hit
CGCTATCACTACTAGTACTCTCGGGGGGCAAAGACATAATGGATTCCCAC2340.15602288335622558No Hit
CGCTATCACTACTAGTACTCTCATGCCCAAGCAAAAAGTGGCTGGCCCTC2310.1540225899798637No Hit
CGCTATCACTACTAGTACTCTCAATCCCAACTGGAAGTCAAAACAACACT2290.15268906106228913No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACACTAATGTTGTTGCTGGA2260.1506887676859273No Hit
CGCTATCACTACTAGTACTCTCCATCAACAGTCACTGGATTGCAGCTGCC2200.14668818093320354No Hit
CGCTATCACTACTAGTACTCTCCAGCACCTGGGTGAATCACACATATGTT2180.14535465201562897No Hit
CGCTATCACTACTAGTACTCTCAGTCCAAACTGGAAGTCAAAACAACACT2100.14002053634533065No Hit
CGCTATCACTACTAGTACTCTCGAAAAGGGAAGGTTACTAAATCAATAGA2080.13868700742775608No Hit
CGCTATCACTACTAGTACTCTCGGGCCATCGGTCATTATGGTGAAACATG2040.1360199495926069No Hit
CGCTATCACTACTAGTACTCTCTAGGCAGCCCTCTGACTAACTCAACCCA2020.13468642067503234No Hit
CGCTATCACTACTAGTACTCTCAGGCAAATGGTAACAACCTCCATGGCCT2010.13401965621624506No Hit
CGCTATCACTACTAGTACTCTCAAAACTATTCTCTGTCGTCTTCAATTTA1990.13268612729867046No Hit
CGCTATCACTACTAGTACTCTCCTATTGTGACTGGGTGTACGTTCTGGAA1980.13201936283988316No Hit
CGCTATCACTACTAGTACTCTCAACCCCCTTTCTAAGTCTGTTACTTTTG1970.1313525983810959No Hit
CGCTATCACTACTAGTACTCTCGTTTGCTCCATCAACAGTCACTGGATTG1960.1306858339223086No Hit
CGCTATCACTACTAGTACTCTCTCTATCATCCCGTCAGGCCCCCTCAAAG1870.124684953793223No Hit
CGCTATCACTACTAGTACTCTCCCGTCAGGCCCCCTCAAAGCCGAGATCG1870.124684953793223No Hit
CGCTATCACTACTAGTACTCTCCAGCAACAACACTGGAATATGCAACCAA1860.12401818933443572No Hit
CGCTATCACTACTAGTACTCTCAAAGACTCCCCGCCCCTGGAAGGACACT1850.12335142487564844No Hit
CGCTATCACTACTAGTACTCTCGAAAAGGGAAAGGTTACTAAATCAATAG1820.12135113149928656No Hit
CGCTATCACTACTAGTACTCTCCTGCGCGTGATCAGCAAAAGCAGGTAGA1750.11668378028777554No Hit
CGCTATCACTACTAGTACTCTCGCCACAAAAAGAAATGCTGCTCCCACTA1630.10868260678232808No Hit
CGCTATCACTACTAGTACTCTCCGAGGGCGAAACGTACGTTCTCTCTATC1620.10801584232354078No Hit
CGCTATCACTACTAGTACTCTCAGGCACATGGTAACAACAACCTCCATGG1600.10668231340596622No Hit
CGCTATCACTACTAGTACTCTCCTGCAACAACACTAATGTTGTTGCTGGA1580.10534878448839163No Hit
CGCTATCACTACTAGTACTCTCCATCCCGTCAGGCCCCCTCAAAGCCGAG1580.10534878448839163No Hit
CGCTATCACTACTAGTACTCTCAAAAGGGGAAGGTTACTAAATCAATAGA1570.10468202002960433No Hit
CGCTATCACTACTAGTACTCTCCAGCGACAACATTAGTGTTGTTAATATT1560.10401525557081706No Hit
CGCTATCACTACTAGTACTCTCCTGCAACAACATTAGTGTTGTTAATATT1540.10268172665324247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTATC201.7133627E-595.282951
CGCTAGC500.095.2829361
TATCACG201.7391392E-594.9974
ACTGCTA201.7391392E-594.9978
GCTATCC156.607442E-494.9972
ACTACGA156.607442E-494.9978
TATAACT301.2249075E-894.9974
CTATCAA254.601825E-794.9969943
ATACTAG254.601825E-794.9969949
CTACTAG149700.093.220179
ACTACTA149450.093.121848
CGCTATC150250.093.095081
CACTACT149750.093.062177
GCTATCA150400.092.849462
TCACTAC149850.092.8415766
CTATCAC150200.092.720113
TATCACT151200.092.0126344
ATCACTA152800.091.142415
GCTAGCA550.086.36092
CGATCAC409.458745E-1083.1223753
Produced by FastQC (version 0.11.5)