FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC106CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC106CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311769
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACA32661.0475704768594698No Hit
CGACTATACGTCACGTGCTGTAGGAGGGGCAAGAGTTCTGCTGAGGACAC18580.595954055727157No Hit
CGACTATACGTCACGTGCTGTACCCAGACCAATCAGTTATTGCTACAACA17360.5568225192369992No Hit
CGACTATACGTCACGTGCTGTACCCTGACCAATCAGTTATTGCTACAACA16880.5414265048802158No Hit
CGACTATACGTCACGTGCTGTACCCCCATTCGTTTCTGATAGGCCTGTAA12780.4099188822493577No Hit
CGACTATACGTCACGTGCTGTACGCGTGATCAGTAGAAACAAGGTCGTTT11620.3727118475537978No Hit
CGACTATACGTCACGTGCTGTAGGGGGGCAAACGGAGTAAAAGGGTTTTC9940.31882579730505595No Hit
CGACTATACGTCACGTGCTGTAGAGGGGGCAAACGGAGTAAAAGGGTTTT9760.3130522919212622No Hit
CGACTATACGTCACGTGCTGTAGATGGAGCAAACGGAGTAAAAGGGTTTT9660.3098447889302657No Hit
CGACTATACGTCACGTGCTGTAGAGGGCAGCTGCAATCCAGTGACTGTTG8690.2787320099175992No Hit
CGACTATACGTCACGTGCTGTAGGGGAGCAAACGGAGTAAAAGGGTTTTC8370.26846800034641033No Hit
CGACTATACGTCACGTGCTGTAGGGGGAGCAAACGGAGTAAAAGGGTTTT7960.2553172380833245No Hit
CGACTATACGTCACGTGCTGTATGGAGCAAACGGAGTAAAAGGGTTTTCA6540.20977069561117367No Hit
CGACTATACGTCACGTGCTGTAGAGGGGCAAACGGAGTAAAAGGGTTTTC6470.20752544351747607No Hit
CGACTATACGTCACGTGCTGTAGGGGGGGCAACGAGATCCATTCTGTGGT6200.19886518544178544No Hit
CGACTATACGTCACGTGCTGTAACGAAAACGTTCTAATCTCTTGTTCCAC6110.19597843274988852No Hit
CGACTATACGTCACGTGCTGTAGGGGAGCGAACGGAGTAAAAGGGTTTTC5840.18731817467419787No Hit
CGACTATACGTCACGTGCTGTACTCCCCTGGAATTCAAGCAAAACTGTAA5510.1767334148039093No Hit
CGACTATACGTCACGTGCTGTACTGACAGCAATCTACTCCCCTGGAATTC5430.17416741241111208No Hit
CGACTATACGTCACGTGCTGTACCCAGAAGCAAGGTCTTATACAGTCCAA5100.1635826525408235No Hit
CGACTATACGTCACGTGCTGTAGAAAGGGGAAAAAGCTAATGTGCTAATT5050.16197890104532522No Hit
CGACTATACGTCACGTGCTGTAAAGGGGAAAAAGCTAATGTGCTAATTGG4860.1558846453624318No Hit
CGACTATACGTCACGTGCTGTAGAGGGAGCAAACGGAGTAAAAGGGTTTT4790.15363939326873424No Hit
CGACTATACGTCACGTGCTGTACGCGTGATCAGTAGAAACAAGGCCGTTT4700.15075264057683735No Hit
CGACTATACGTCACGTGCTGTAGGAGGAGCAAACGGAGTAAAAGGGTTTT4590.14722438728674114No Hit
CGACTATACGTCACGTGCTGTAGGAAGGGGAAAAAGCTAATGTGCTAATT4420.14177163220204703No Hit
CGACTATACGTCACGTGCTGTAACGCCACAAAAAGAAATGCTGCTCCCAC4370.14016788070654876No Hit
CGACTATACGTCACGTGCTGTAAAAGGGGAAAAAGCTAATGTGCTAATTG4320.1385641292110505No Hit
CGACTATACGTCACGTGCTGTACCAACACTACATCCCCTTGCCCAATTAG4230.13567737651915363No Hit
CGACTATACGTCACGTGCTGTACGCGTGATCAGCAAAAGCAGGTGACTCA4180.13407362502365533No Hit
CGACTATACGTCACGTGCTGTAAGGGGAAAAAGCTAATGTGCTAATTGGG3990.1279793693407619No Hit
CGACTATACGTCACGTGCTGTAGGAGGGCCAAGAGTTCTGCTGAGGACAC3970.12733786874256262No Hit
CGACTATACGTCACGTGCTGTAGGGGGAAAAAGCTAATGTGCTAATTGGG3550.11386635618037713No Hit
CGACTATACGTCACGTGCTGTAAAAGGGTTTTCATACAAATATGATAATG3480.11162110408667957No Hit
CGACTATACGTCACGTGCTGTATCAGTAGAAACAAGGTCGTTTTTAAATT3470.11130035378757991No Hit
CGACTATACGTCACGTGCTGTATGGGGACGGAGCTTCACCTAGAGGACAG3410.109375851992982No Hit
CGACTATACGTCACGTGCTGTACGCGTGATCAGCAAAAGCAGGGGTAACT3390.10873435139478267No Hit
CGACTATACGTCACGTGCTGTACATCCCCTTGCCCAATTAGCACATTAGC3350.10745135019838406No Hit
CGACTATACGTCACGTGCTGTAGGGGGGGCAAACGGAGTAAAAGGGTTTT3340.1071305998992844No Hit
CGACTATACGTCACGTGCTGTAGAAACAAGGTCGTTTTTAAATTATTCGA3330.10680984960018475No Hit
CGACTATACGTCACGTGCTGTAAGGGGGCAAACGGAGTAAAAGGGTTTTC3240.10392309690828788No Hit
CGACTATACGTCACGTGCTGTATTGGGCAAGGGGATGTAGTGTTGGTAAT3230.10360234660918821No Hit
CGACTATACGTCACGTGCTGTAAGACCCTTTTCTATACTGACACCTCCTG3210.1029608460109889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT156.6140306E-494.997595
ATTCGTC254.613412E-794.997596
TTCGTCA254.613412E-794.997597
ATATGTC450.094.997596
TTATACG550.094.997594
TATTCGT254.613412E-794.997595
ACGTCGC353.2923708E-1094.997598
ATGCGTC254.613412E-794.997596
CGTAACG254.613412E-794.997599
CTACACG156.6140306E-494.997594
ATACGGC750.094.997596
CGTCATG254.613412E-794.997599
CGATACG156.6140306E-494.997594
TACACGT156.6140306E-494.997595
GTACGTC650.094.997596
TACGTCT254.613412E-794.997597
TACGTCG353.2923708E-1094.997597
CGACTAT301900.094.942811
ATACGTC303850.094.825646
TACGTCA303600.094.82557
Produced by FastQC (version 0.11.5)