FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC099CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC099CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332868
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT220146.613432351562781No Hit
CGTACGTCTACTACTCTACTGTATGAGCTACTTGGGAAGAATTTTTCAAA34471.0355456216878762No Hit
CGTACGTCTACTACTCTACTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC28390.8528906353269163No Hit
CGTACGTCTACTACTCTACTGTAGGCCACATGGCCATAATCAAGAAATAC27970.8402730211375079No Hit
CGTACGTCTACTACTCTACTGTCGGCCCCCTCAAAGCCGAGATCGCACAG24980.7504476248843386No Hit
CGTACGTCTACTACTCTACTGTCATCCCGTCAGGCCCCCTCAAAGCCGAG20650.620366030979247No Hit
CGTACGTCTACTACTCTACTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG18080.543158248915486No Hit
CGTACGTCTACTACTCTACTGTAGACCACATGGCCATAATCAAGAAATAC17500.5257339245586838No Hit
CGTACGTCTACTACTCTACTGTCGTCCCGTCAGGCCCCCTCAAAGCCGAG16630.4995974380234807No Hit
CGTACGTCTACTACTCTACTGTCTTCCCGATGTGTATTTCTTGATTATGG11960.35930158501267767No Hit
CGTACGTCTACTACTCTACTGTAATGCTTGCATAAATGTTATTTGCTCAA10450.31393825780789986No Hit
CGTACGTCTACTACTCTACTGTAGCCCACATGGCCATAATCAAGAAATAC9400.2823942223343788No Hit
CGTACGTCTACTACTCTACTGTCAGCCCCCTCAAAGCCGAGATCGCACAG8610.2586610908828725No Hit
CGTACGTCTACTACTCTACTGTCTTCCTGATGTGTATTTCTTGATTATGG8610.2586610908828725No Hit
CGTACGTCTACTACTCTACTGTAGGCCCCCTCAAAGCCGAGATCGCACAG8530.25625773579917566No Hit
CGTACGTCTACTACTCTACTGTCGAAACGTACGTTCTCTCTATCATCCCG8000.2403355083696841No Hit
CGTACGTCTACTACTCTACTGTGAGGGCGAAACGTACGTTCTCTCTATCA7330.22020740954372303No Hit
CGTACGTCTACTACTCTACTGTCAGACCCCCTCAAAGCCGAGATCGCACA6970.20939231166708727No Hit
CGTACGTCTACTACTCTACTGTAGCCACATGGCCATAATCAAGAAATACA6930.20819063412523883No Hit
CGTACGTCTACTACTCTACTGTCGAGGGCGAAACGTACGTTCTCTCTATC6620.19887763317591356No Hit
CGTACGTCTACTACTCTACTGTCAGCCCGTCAGGCCCCCTCAAAGCCGAG6290.1889637934556641No Hit
CGTACGTCTACTACTCTACTGTCAGCCCCCCTCAAAGCCGAGATCGCACA6260.1880625352992778No Hit
CGTACGTCTACTACTCTACTGTAACCACATGGCCATAATCAAGAAATACA5850.17574534049533147No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGTAGAAACAAGGTAGTT5460.16402898446230937No Hit
CGTACGTCTACTACTCTACTGTCATCGCGTCAGGCCCCCTCAAAGCCGAG5190.15591766105483254No Hit
CGTACGTCTACTACTCTACTGTCGACCACATGGCCATAATCAAGAAATAC5120.1538147253565978No Hit
CGTACGTCTACTACTCTACTGTCGGCAACCTGTGAACAGATTGCCGACTC5080.15261304781474938No Hit
CGTACGTCTACTACTCTACTGTGAGTCTCTTGGTAGTCTTGTTGTAGTTG4810.14450172440727257No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGTAGAAACAAGGTACTT4770.14330004686542414No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGTAGAAACAAGGTCGTT4320.1297811745196294No Hit
CGTACGTCTACTACTCTACTGTCTGCCCCCTCAAAGCCGAGATCGCACAG3900.11716356033022099No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGCAAAAGCAGGTAGATA3880.11656272155929678No Hit
CGTACGTCTACTACTCTACTGTCACCTCTGACTAAGGGGATTTTAGGATT3680.11055433385005467No Hit
CGTACGTCTACTACTCTACTGTAAACCACATGGCCATAATCAAGAAATAC3520.10574762368266098No Hit
CGTACGTCTACTACTCTACTGTCAGGCCCCCTCAAAGCCGAGATCGCACA3490.10484636552627467No Hit
CGTACGTCTACTACTCTACTGTCAGGGCCCCTCAAAGCCGAGATCGCACA3470.10424552675535045No Hit
CGTACGTCTACTACTCTACTGTATACCACATGGCCATAATCAAGAAATAC3470.10424552675535045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAT254.5122033E-795.354351
CATACGT450.095.354341
AACGTCT156.6148E-494.996253
ACGCCTA950.094.996254
ACGTTTA201.742344E-594.996254
CGTCTAG500.094.996255
GTCTAGT500.094.996256
CTAGTAC500.094.996258
CTACGAC301.2296368E-894.996258
GAACGTC156.6148E-494.996252
TTACGTC156.6148E-494.996252
CGTTTAC201.742344E-594.996255
TACGTCA254.614485E-794.996253
GTCGACT550.094.996246
TACGTCT326650.094.429153
GTACGTC327000.094.40072
ACGTCTA326050.094.398974
CTACTAC327550.094.3726968
TACTACT328300.094.301789
GTCTACT327550.094.169686
Produced by FastQC (version 0.11.5)