FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC082CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC082CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302242
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGATCGTCTCATACGTAGCCCCCCAAGATCAAAGGTCCCAGGTTCCA38571.2761297238636589No Hit
CGTAGATCGTCTCATACGTAGCAGGCACTGATTCAAAATGGAAGATTTTG31891.0551147755771866No Hit
CGTAGATCGTCTCATACGTAGCCCCCGAAGATCAAAGGTCCCAGGTTCCA29250.9677675505058859No Hit
CGTAGATCGTCTCATACGTAGCCGCCCAAGATCAAAGGTCCCAGGTTCCA20700.6848816511272424No Hit
CGTAGATCGTCTCATACGTAGCCCACCAAGATCAAAGGTCCCAGGTTCCA19700.651795581024477No Hit
CGTAGATCGTCTCATACGTAGCCCCACAAGATCAAAGGTCCCAGGTTCCA19520.6458400884059793No Hit
CGTAGATCGTCTCATACGTAGCCCCCAAGATCAAAGGTCCCAGGTTCCAG19210.635583406674122No Hit
CGTAGATCGTCTCATACGTAGCACCCCAAGATCAAAGGTCCCAGGTTCCA17220.569742127169619No Hit
CGTAGATCGTCTCATACGTAGCCCCCTAAGATCAAAGGTCCCAGGTTCCA17010.5627940524480384No Hit
CGTAGATCGTCTCATACGTAGCCACCCAAGATCAAAGGTCCCAGGTTCCA16050.5310314251493836No Hit
CGTAGATCGTCTCATACGTAGCCTCCCAAGATCAAAGGTCCCAGGTTCCA13990.462874120737687No Hit
CGTAGATCGTCTCATACGTAGCAGGCCCTCTTTTTAAACCGTGTTTAAAG13550.44831624989247026No Hit
CGTAGATCGTCTCATACGTAGCCCTCCAAGATCAAAGGTCCCAGGTTCCA13170.43574354325341946No Hit
CGTAGATCGTCTCATACGTAGCCCGCCAAGATCAAAGGTCCCAGGTTCCA12570.4158919011917603No Hit
CGTAGATCGTCTCATACGTAGCAGGACACTGTTGGCTAAGTCAGTATTCA11700.3871070202023544No Hit
CGTAGATCGTCTCATACGTAGCAGCAAAAGCAGGTACTGATTCAAAATGG11050.3656010746355569No Hit
CGTAGATCGTCTCATACGTAGCAAAAGCAGGTACTGATTCAAAATGGAAG9800.32424348700710026No Hit
CGTAGATCGTCTCATACGTAGCAGGCCAATTATATTCAATATGGAAAGAA9780.323581765605045No Hit
CGTAGATCGTCTCATACGTAGCAGAAAAAGCAATGAAAGAGTATGGAGAG7850.25972565030670786No Hit
CGTAGATCGTCTCATACGTAGCCGTCCCTCATAATCATTGAGCTTAAGCT7360.24351347595635287No Hit
CGTAGATCGTCTCATACGTAGCCCCCCCAGATCAAAGGTCCCAGGTTCCA7180.2375579833378551No Hit
CGTAGATCGTCTCATACGTAGCCCCAGCTATCTCAATGCATGTGTTAGGA7090.23458023702860623No Hit
CGTAGATCGTCTCATACGTAGCCTAAAATGAGGAATCCCCTTAGAACCGC6870.22730130160599785No Hit
CGTAGATCGTCTCATACGTAGCCACAAAAAGAAATGCTGCTCCCACTAGT6860.2269704409049702No Hit
CGTAGATCGTCTCATACGTAGCGAAGGGGGAAAAAGCTAATGTGCTAATT6750.223330973193666No Hit
CGTAGATCGTCTCATACGTAGCAGGTACTGATTCAAAATGGAAGATTTTG6510.21539031636900233No Hit
CGTAGATCGTCTCATACGTAGCCGTCCAGATTCGAAATCAATCCGTGCAT6430.2127434307607811No Hit
CGTAGATCGTCTCATACGTAGCGGAAGGGGAAAAAGCTAATGTGCTAATT5980.1978546992145367No Hit
CGTAGATCGTCTCATACGTAGCAGGCAAACCATTTGAATGGATGTCAATC5950.19686211711145374No Hit
CGTAGATCGTCTCATACGTAGCCAGCACTACAGCTAAGGCTATGGAGCAA5690.18825973888473474No Hit
CGTAGATCGTCTCATACGTAGCGGGAGGGGAAAAAGCTAATGTGCTAATT5540.18329682836931996No Hit
CGTAGATCGTCTCATACGTAGCCCCCAAAGATCAAAGGTCCCAGGTTCCA5100.1687389575241032No Hit
CGTAGATCGTCTCATACGTAGCAGTCAAACTTTATCGAAAGCTTAAGAGG5040.16675379331793727No Hit
CGTAGATCGTCTCATACGTAGCCGGCCCTCTTTTTAAACCGTGTTTAAAG5040.16675379331793727No Hit
CGTAGATCGTCTCATACGTAGCGGAGGGGGAAAAAGCTAATGTGCTAATT4990.165099489812799No Hit
CGTAGATCGTCTCATACGTAGCGAGAGGGGAAAAAGCTAATGTGCTAATT4950.1637760470086884No Hit
CGTAGATCGTCTCATACGTAGCACCCCCATTCGTTTCTGATAGGCCTGTA4930.16311432560663308No Hit
CGTAGATCGTCTCATACGTAGCGAGGGGGGAAAAAGCTAATGTGCTAATT4910.1624526042045778No Hit
CGTAGATCGTCTCATACGTAGCATGCCCCAGCTATCTCAATGCATGTGTT4830.15980571859635656No Hit
CGTAGATCGTCTCATACGTAGCAGGCAAATGGTAACAACAACCAATCCAT4800.15881313649327358No Hit
CGTAGATCGTCTCATACGTAGCAGGCGCTGATTCAAAATGGAAGATTTTG4780.1581514150912183No Hit
CGTAGATCGTCTCATACGTAGCCACTCCATGAGAGCCTCAAGATCGGTAT4710.15583539018402473No Hit
CGTAGATCGTCTCATACGTAGCGGGCAGAAAAAGCAATGAAAGAGTATGG4700.15550452948299706No Hit
CGTAGATCGTCTCATACGTAGCGAAAGGGGAAAAAGCTAATGTGCTAATT4560.15087247966860992No Hit
CGTAGATCGTCTCATACGTAGCCAGCACAAAATGCTATAAGCACAACTTT4320.14293182284394623No Hit
CGTAGATCGTCTCATACGTAGCAGGCCCATTATATTCAATATGGAAAGAA3890.12870481269975717No Hit
CGTAGATCGTCTCATACGTAGCCACACCATGAGAGCCTCAAGATCGGTAT3840.12705050919461888No Hit
CGTAGATCGTCTCATACGTAGCAAGGCCGTTTTTAAATTATTCGAAATTA3770.12473448428742531No Hit
CGTAGATCGTCTCATACGTAGCACGCGTGATCAGTAGAAACAAGGTCGTT3750.12407276288537No Hit
CGTAGATCGTCTCATACGTAGCGGCAGAAAAAGCAATGAAAGAGTATGGA3700.12241845938023173No Hit
CGTAGATCGTCTCATACGTAGCATCCCCTTGCCCAATTAGCACATTAGCT3690.12208759867920409No Hit
CGTAGATCGTCTCATACGTAGCGCAAGGGGAAAAAGCTAATGTGCTAATT3600.11910985236995521No Hit
CGTAGATCGTCTCATACGTAGCAGAAACGAATGGGGGTGCAGATGCAACG3550.11745554886481693No Hit
CGTAGATCGTCTCATACGTAGCGAGGGGGAAAAAGCTAATGTGCTAATTG3540.11712468816378928No Hit
CGTAGATCGTCTCATACGTAGCAAAGGGGAAAAAGCTAATGTGCTAATTG3530.11679382746276164No Hit
CGTAGATCGTCTCATACGTAGCCACCCCATGAGAGCCTCAAGATCGGTAT3510.11613210606070633No Hit
CGTAGATCGTCTCATACGTAGCAAAAGCAGGTAGATATTGAAAGATGAGT3440.11381608115351276No Hit
CGTAGATCGTCTCATACGTAGCCAAAGGGGAAAAAGCTAATGTGCTAATT3410.11282349905042978No Hit
CGTAGATCGTCTCATACGTAGCAACAACCAATCCATTAATAAGACATGAG3370.11150005624631917No Hit
CGTAGATCGTCTCATACGTAGCGCGCAGAAAAAGCAATGAAAGAGTATGG3330.11017661344220855No Hit
CGTAGATCGTCTCATACGTAGCGGAGGGGAAAAAGCTAATGTGCTAATTG3310.10951489204015326No Hit
CGTAGATCGTCTCATACGTAGCAAAAGGGGAAAAAGCTAATGTGCTAATT3260.10786058853501498No Hit
CGTAGATCGTCTCATACGTAGCAGGAAAGCAATGAAAGAGTATGGAGAGG3210.10620628502987672No Hit
CGTAGATCGTCTCATACGTAGCAGAAGGGGAAAAAGCTAATGTGCTAATT3200.10587542432884908No Hit
CGTAGATCGTCTCATACGTAGCCATCCGAATTCTTTTGGTCGCTGTCTGG3190.1055445636278214No Hit
CGTAGATCGTCTCATACGTAGCGAAGGGGAAAAAGCTAATGTGCTAATTG3170.1048828422257661No Hit
CGTAGATCGTCTCATACGTAGCAGAAGCAGGTACTGATTCAAAATGGAAG3040.1005816531124066No Hit
CGTAGATCGTCTCATACGTAGCTTGCAGAAAAAGCAATGAAAGAGTATGG3040.1005816531124066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT450.095.265141
AACGTCT700.094.997196
TAGACCG600.094.997193
GACCGTC600.094.997195
TATATCG700.094.997193
CGTCTAA600.094.997198
CCGTCTC600.094.997197
ATATCGT700.094.997194
GTGTCAT850.094.997199
TCGTCTG950.094.997197
TCGTCTA600.094.997197
GAACGTC700.094.997195
AGTTCGT650.094.997194
GTATATC700.094.997192
TAGATCC156.6139444E-494.997193
ATCGACT254.6131754E-794.997196
GTCTCAT300500.092.12049
CGTAGAT297950.092.0997541
CGTCTCA299500.092.0314948
ATCGTCT299900.091.892916
Produced by FastQC (version 0.11.5)