FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC080CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC080CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44907
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACGACTACGCGCTCACTAACCGAGGTCGAAACGTACGTTCTTTCT4250.9464003384772977No Hit
CGACGACGACTACGCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCT3440.7660275680851538No Hit
CGACGACGACTACGCGCTCACTAGACCAAAAGCAGCTTCTGTGGTCACTG3060.6814082437036543No Hit
CGACGACGACTACGCGCTCACTCAAAGCCGAGATCGCGCAGAGACTGGAA2370.5277573652214577No Hit
CGACGACGACTACGCGCTCACTCGATCCAGCCATCTGTTCCATAGCCTTT2150.4787672300532211No Hit
CGACGACGACTACGCGCTCACTGGTGGGGAGCGCCAGGATGCCACAGAAA2120.4720867570757343No Hit
CGACGACGACTACGCGCTCACTGTTCCCATCCTGTTGTATATGAGGCCCA1670.37187966241343223No Hit
CGACGACGACTACGCGCTCACTAAGCTATTCAACTGGTGCACTTGCCAGT1670.37187966241343223No Hit
CGACGACGACTACGCGCTCACTGTGCCCAGTGAGCGAGGACTGCAGCGTA1350.30062128398690624No Hit
CGACGACGACTACGCGCTCACTAGGCAAAAGATAATAGGCCCTCTTTGCG1240.276126216402788No Hit
CGACGACGACTACGCGCTCACTTGGGTAAATCAGACATATGTTAACATCA1150.2560847974703276No Hit
CGACGACGACTACGCGCTCACTTGAATCGCTGCATCTGCACTCCCATTCG1130.2516311488186697No Hit
CGACGACGACTACGCGCTCACTCCAACACCATGTCAAGCTTTCAGGTAGA1110.24717750016701184No Hit
CGACGACGACTACGCGCTCACTATTCGCTTCTGGTAGGCCTGCAAATTTT1070.23827020286369607No Hit
CGACGACGACTACGCGCTCACTGTAGAGACCCATTAGAGCACATCCAGAA1000.22268243258289355No Hit
CGACGACGACTACGCGCTCACTCGAAGCCGAGATCGCGCAGAGACTGGAA960.2137751352795778No Hit
CGACGACGACTACGCGCTCACTAGACGATACTGGACCACAACTGCCTGTC940.20932148662791994No Hit
CGACGACGACTACGCGCTCACTCAGCAAAAGCAGGTAGATATTTAAAGAT940.20932148662791994No Hit
CGACGACGACTACGCGCTCACTGTTTACACCACAAAAGGATATGCTGCTC860.19150689202128846No Hit
CGACGACGACTACGCGCTCACTGGAGCTAGGATGAGTCCCAATAGTTCTC850.18928006769545952No Hit
CGACGACGACTACGCGCTCACTAGTAGAGCCGGGAGACAAAATAACATTC760.16923864876299907No Hit
CGACGACGACTACGCGCTCACTAGGCACCAAACGATCATATGAACAAATG760.16923864876299907No Hit
CGACGACGACTACGCGCTCACTGGTGGTGAGCGCCAGGATGCCACAGAAA750.16701182443717014No Hit
CGACGACGACTACGCGCTCACTCAGCACCGTCTGGCCAAGACCAACCCAC720.16033135145968336No Hit
CGACGACGACTACGCGCTCACTAGAATCAGGATAACAGGAGCATTCCTCA660.14697040550470974No Hit
CGACGACGACTACGCGCTCACTTGGGAATCAAAATCAGATTGAAACATGC650.1447435811788808No Hit
CGACGACGACTACGCGCTCACTCAGCAAAAGCAGGGTGACAAAAACATAA650.1447435811788808No Hit
CGACGACGACTACGCGCTCACTCCCATTCGCTTCTGGTAGGCCTGCAAAT610.13583628387556507No Hit
CGACGACGACTACGCGCTCACTGGGGCCAAGGAGGTGTCACTAAGCTATT600.13360945954973613No Hit
CGACGACGACTACGCGCTCACTGGGGGACAAAAACATAATGGACTCCAAC600.13360945954973613No Hit
CGACGACGACTACGCGCTCACTACGCGTGATCAGTAGAAACAAGGTAGTT580.12915581089807823No Hit
CGACGACGACTACGCGCTCACTACGGCAAAGGCTATGGAACAGATGGCTG580.12915581089807823No Hit
CGACGACGACTACGCGCTCACTAAGACCACTGTGGACCATATGGCCATAA570.1269289865722493No Hit
CGACGACGACTACGCGCTCACTGGAGGTAGTGAAGGTCTCCCATTCTCAT560.12470216224642038No Hit
CGACGACGACTACGCGCTCACTGGGATGCTCTTCTAGGTATTTATTTCTT550.12247533792059144No Hit
CGACGACGACTACGCGCTCACTACGCGTGATCAGCAAAAGCAGGTAGATA530.11802168926893357No Hit
CGACGACGACTACGCGCTCACTGTTGACAAAATGACCATCGTCAACATCC530.11802168926893357No Hit
CGACGACGACTACGCGCTCACTGGGGGGGAGCGCCAGGATGCCACAGAAA480.1068875676397889No Hit
CGACGACGACTACGCGCTCACTAGACCCAAAGCAGCTTCTGTGGTCACTG480.1068875676397889No Hit
CGACGACGACTACGCGCTCACTGGGGGGCCAAGGAGGTGTCACTAAGCTA460.10243391898813103No Hit
CGACGACGACTACGCGCTCACTGGGGAGGGAACAATAGTTGGAGAAATTT460.10243391898813103No Hit
CGACGACGACTACGCGCTCACTACCACCAATCCACTAATCAGGCATGAAA460.10243391898813103No Hit
CGACGACGACTACGCGCTCACTCATGGACTCAGGCACTCCTTCCGTAGAA450.1002070946623021No Hit
CGACGACGACTACGCGCTCACTAAGCAAGATATCGTAGGAATAAATGAGT450.1002070946623021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGACG156.5771415E-494.996662
GACGACT47450.089.0906455
ACGACTA47300.089.071926
CGACTAC47200.089.0593647
GACTACG47150.088.952338
ACTACGC47450.088.189739
GACGACG50800.082.748072
ACGACGA51000.082.423573
AGGACGA200.002061367771.24753
TGACGAC200.002061367771.24754
CGATGAC250.00492490857.1890531
GATGACG250.00499068456.9979932
CTACAGA252.8066444E-547.4983320-21
CTACAAG357.8141966E-847.4983320-21
CTTCAGA205.362455E-447.4983330-31
ACTGCGG252.8066444E-547.4983320-21
ATGGGGA252.8066444E-547.4983380-81
ACTGCCC205.362455E-447.4983320-21
ACTAACG205.362455E-447.4983320-21
ACTCGGC452.2191671E-1047.4983320-21
Produced by FastQC (version 0.11.5)