FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC078CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC078CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24625
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACGTAGCAGCACGACGAAAGCTCAAGAGGGAGATAACATTCCATG1210.49137055837563454No Hit
CGACGACGTAGCAGCACGACGAAAACGTTCTAATCTCTTGTTCCACTTCA940.3817258883248731No Hit
CGACGACGTAGCAGCACGACGAAAAAGCAATGAAAGAGTATGGAGAGGAC900.36548223350253806No Hit
CGACGACGTAGCAGCACGACGAACCCAAATCAAAAGATAATAACCATTGG820.33299492385786805No Hit
CGACGACGTAGCAGCACGACGACAGAGCAGTCAAACTTTATCGAAAGCTT770.31269035532994927No Hit
CGACGACGTAGCAGCACGACGAACGCCACAAAAAGAAATGCTGCTCCCAC620.2517766497461929No Hit
CGACGACGTAGCAGCACGACGAGGGAGATAACATTCCATGGGGCCAAAGA590.23959390862944163No Hit
CGACGACGTAGCAGCACGACGAAATCGAAAGCTTAAGAGGGAGATAACAT560.22741116751269036No Hit
CGACGACGTAGCAGCACGACGAGAGGGGTTTGAGATGATTTGGGATCCTA500.20304568527918782No Hit
CGACGACGTAGCAGCACGACGAAGGCCCTCTTTTTAAACCGTGTTTAAAG470.19086294416243654No Hit
CGACGACGTAGCAGCACGACGAATGAGAAAATGTGAATGTGTGTCTTCTC470.19086294416243654No Hit
CGACGACGTAGCAGCACGACGAAAATTGGGTGCATTCAACTCTATTGATT450.18274111675126903No Hit
CGACGACGTAGCAGCACGACGAATCAGCATTTGGCCTTATATGTGCAACC420.17055837563451776No Hit
CGACGACGTAGCAGCACGACGACGCCACAAAAAGAAATGCTGCTCCCACT400.16243654822335024No Hit
CGACGACGTAGCAGCACGACGAAGCACCGTCTGGCCAAGACCAGTTTGCA400.16243654822335024No Hit
CGACGACGTAGCAGCACGACGAAGGGGTTTGAGATGATTTGGGATCCTAA390.1583756345177665No Hit
CGACGACGTAGCAGCACGACGAAGGACCAAAAGTAACAGACTTAGAAAGG380.15431472081218275No Hit
CGACGACGTAGCAGCACGACGATCCAGCCATTTGCTCCATAGCCTTAGCT380.15431472081218275No Hit
CGACGACGTAGCAGCACGACGAATGGGAGTGCAGATGCAACAACAAGAGG360.14619289340101524No Hit
CGACGACGTAGCAGCACGACGAACAAGCAGCTGAGGCCATGGAGGTTGCT340.1380710659898477No Hit
CGACGACGTAGCAGCACGACGAGATCGGAAGAGCACACGTCTGAACTCCA330.13401015228426397Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACGACGTAGCAGCACGACGACTCAGCACCGTCTGGCCAAGACCAGTTT320.1299492385786802No Hit
CGACGACGTAGCAGCACGACGACACAGAAACGAAAACGTTCTAATCTCTT320.1299492385786802No Hit
CGACGACGTAGCAGCACGACGAATGGGAGTGCAGATGCAACGATTCAAGT300.1218274111675127No Hit
CGACGACGTAGCAGCACGACGAGGGGTTTGAGATGATTTGGGATCCTAAT300.1218274111675127No Hit
CGACGACGTAGCAGCACGACGACACTGAAACGAAAACGTTCTAATCTCTT290.11776649746192892No Hit
CGACGACGTAGCAGCACGACGACCCAGAAGCAAGGTCTTATACAGTCCAA290.11776649746192892No Hit
CGACGACGTAGCAGCACGACGAGGGGGGGCAAAGACATAATGGATTCCCA280.11370558375634518No Hit
CGACGACGTAGCAGCACGACGATATTGAAAGATGAGTCTTCTAACCGAGG270.10964467005076142No Hit
CGACGACGTAGCAGCACGACGAAAGAGGGAAATAACATTCCATGGGGCCA270.10964467005076142No Hit
CGACGACGTAGCAGCACGACGAGAGGGCAGCTGCAATCCAGTGACTGTTG270.10964467005076142No Hit
CGACGACGTAGCAGCACGACGAGAGGGAGATAACATTCCATGGGGCCAAA260.10558375634517765No Hit
CGACGACGTAGCAGCACGACGACACAAAAAGAAATGCTGCTCCCACTAGT260.10558375634517765No Hit
CGACGACGTAGCAGCACGACGAAAGCACAGATTTGAGATAATAGAGGGAC260.10558375634517765No Hit
CGACGACGTAGCAGCACGACGAAAGGGGTTTGAGATGATTTGGGATCCTA260.10558375634517765No Hit
CGACGACGTAGCAGCACGACGAGGGGCGGGAACAACTAGGTCAGAAGTTT260.10558375634517765No Hit
CGACGACGTAGCAGCACGACGAAAGGAGTCCCAAAGACCTCTGCTTGCCA250.10152284263959391No Hit
CGACGACGTAGCAGCACGACGAACCCGAATTAAGATCATTTTCAAGTTGG250.10152284263959391No Hit
CGACGACGTAGCAGCACGACGAGGTAGATATTGAAAGATGAGTCTTCTAA250.10152284263959391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAGC24400.094.805339
GTAGCAG24450.094.611458
ACGTAGC24350.094.609866
CGTAGCA24350.094.609867
GACGTAG24350.094.609865
CGACGTA24450.094.4171754
ACGACGT24500.094.2244953
GACGACG25550.090.166342
CGACGAC30150.076.094531
ACGAGGA452.1645974E-1047.50000418-19
GACGAAC2650.047.50000418-19
ACGACAC1150.047.50000418-19
CGACCAA452.1645974E-1047.50000420-21
CGACAGA252.7874192E-547.520-21
CGACACA600.047.520-21
AGGGGAG252.7874192E-547.522-23
TCAAGAG301.4631314E-647.526-27
ACGAGCC252.7874192E-547.518-19
ACGAGAG1050.047.518-19
CGAACGA252.7874192E-547.520-21
Produced by FastQC (version 0.11.5)