FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC070CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC070CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92229
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGAGCTACGTCGTGCACGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA4430.48032614470502766No Hit
CGTGAGCTACGTCGTGCACGTAGGGGCGGCAAGATTATTAGCGGTTTTGC3870.41960771557752985No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGAAATGAAGCAGGGGGCTGCGC3720.4033438506326643No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGAGAAGCTTTCAGTCTCGTACCA2890.3133504646044086No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGCTGCGCGCGGCGTAGAAAACTGC2480.26889590042177625No Hit
CGTGAGCTACGTCGTGCACGTAGACGGGGAAATGAAGCAGGGGGCTGCGC2040.22118856325017075No Hit
CGTGAGCTACGTCGTGCACGTAAGAGGGGGAACACGTACAATGCTTGTTT2020.2190200479241887No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGAGGAACACGTACAATGCTTGTTT1790.19408212167539493No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGGAAGCTTCGTGCTGGAGAAAA1790.19408212167539493No Hit
CGTGAGCTACGTCGTGCACGTAGTTGGCGGCAAGATTATTAGCGGTTTTG1770.19191360634941287No Hit
CGTGAGCTACGTCGTGCACGTAATGACCCCGCCACCCAAGATAAAGCGTT1770.19191360634941287No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGGGCAAAACCGCTAATAATCTTGC1620.17564974140454737No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGGGCTAACCCTAATGGCAAGGACT1600.1734812260785653No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGACAAAAACATAATGGACTCCAAC1580.17131271075258325No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAGATTATTAGCGGTTTTGC1570.17022845308959222No Hit
CGTGAGCTACGTCGTGCACGTAGAAAAGCGAAAGCATAGAAAGTGAGTGT1450.15721736113369983No Hit
CGTGAGCTACGTCGTGCACGTACCCCGCCACCCAAGATAAAGCGTTCAGT1430.15504884580771774No Hit
CGTGAGCTACGTCGTGCACGTAGGATGGGCAAAACCGCTAATAATCTTGC1410.15288033048173566No Hit
CGTGAGCTACGTCGTGCACGTACGCGTGATCAGCAAAAGCAGGTGGCACG1370.14854329982977155No Hit
CGTGAGCTACGTCGTGCACGTAGGACGGGGAAATGAAGCAGGGGGCTGCG1270.1377007231998612No Hit
CGTGAGCTACGTCGTGCACGTACTCAAAATCAAATAATAGTTGTAAAGTG1260.13661646553687018No Hit
CGTGAGCTACGTCGTGCACGTAGGCGGGGAAATGAAGCAGGGGGCTGCGC1240.13444795021088812No Hit
CGTGAGCTACGTCGTGCACGTAGAGGCGGCAAGATTATTAGCGGTTTTGC1180.12794240423294193No Hit
CGTGAGCTACGTCGTGCACGTAGGGTGTGTGAGAGTGTGTGTGTATGTGC1170.1268581465699509No Hit
CGTGAGCTACGTCGTGCACGTAGAGGGGCCAAGGAGGTGTCACTAAGCTA1150.12468963124396883No Hit
CGTGAGCTACGTCGTGCACGTACAAGTGGCACACACTAGACCAAAAGCAG1150.12468963124396883No Hit
CGTGAGCTACGTCGTGCACGTAGGGGAGGCAAGAGAAGAACCCCGCACTC1120.12143685825499573No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAGAAGGGGATTGAACTCAG1120.12143685825499573No Hit
CGTGAGCTACGTCGTGCACGTAAACGGGGAAATGAAGCAGGGGGCTGCGC1110.12035260059200469No Hit
CGTGAGCTACGTCGTGCACGTATGTTGACAAAATGACCATCGTCAACATC1050.1138470546140585No Hit
CGTGAGCTACGTCGTGCACGTATTTGGGCATTAATTTCTTTACTTGTTGT1050.1138470546140585No Hit
CGTGAGCTACGTCGTGCACGTACGGCAAAGGCTATGGAACAGATGGCTGG1050.1138470546140585No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAAACCGCTAATAATCTTGC1010.10951002396209435No Hit
CGTGAGCTACGTCGTGCACGTATTGGGAAGAGTTGTCGTTGCTTCATGAC1000.1084257662991033No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGGAAATGAAGCAGGGGGCTGCG940.1019202203211571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGC156.542307E-495.204351
CGGGAGC156.542307E-495.204351
TAGGTCG201.735494E-594.997838
CTACGTA201.735494E-594.997837
GGTACGT156.5990683E-494.9978266
ACGGCGT156.5990683E-494.9978269
CTACGTG301.2196324E-894.9978267
GCTACGG156.5990683E-494.9978266
TACGTGG301.2196324E-894.9978268
TGCGTCG156.5990683E-494.9978268
GCTACGT91100.094.789276
TACGTCG90750.094.736138
ACGTCGT90550.094.68319
CGTGAGC91050.094.472411
TGAGCTA91700.094.272663
AGCTACG91850.094.222125
GTGAGCT91750.094.1177442
CTACGTC91450.093.90717
GAGCTAC95100.090.902234
ACGTGGT353.555033E-881.426719
Produced by FastQC (version 0.11.5)