FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC062CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC062CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90457
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGAGTCGCGAGTGTGAGCGCAGAGACTGGAAAGTGTCTTTGCAGGAA3920.4333550747869153No Hit
CGACGAGTCGCGAGTGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGT3380.3736582022397382No Hit
CGACGAGTCGCGAGTGTGAGCGCGGAGACTGGAAAGTGTCTTTGCAGGAA2610.2885348839780227No Hit
CGACGAGTCGCGAGTGTGAGCGCAGAGCCTGGAAAGTGTCTTTGCAGGAA2570.28411289341897256No Hit
CGACGAGTCGCGAGTGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAA2390.2642139359032469No Hit
CGACGAGTCGCGAGTGTGAGCGCAAAGCAGGTAGATATTTAAAGATGAGT1940.21446654211393257No Hit
CGACGAGTCGCGAGTGTGAGCGAAAAGCAGGTAGATATTTAAAGATGAGT1830.20230606807654467No Hit
CGACGAGTCGCGAGTGTGAGCGTCTGGCTGTCAGTAAGTATGCTAGAGTC1780.19677857987773195No Hit
CGACGAGTCGCGAGTGTGAGCGCGCAGAGACTGGAAAGTGTCTTTGCAGG1760.1945675845982069No Hit
CGACGAGTCGCGAGTGTGAGCGATCAGCAAAAGCAGGTAGATATTTAAAG1570.17356312944271865No Hit
CGACGAGTCGCGAGTGTGAGCGCTGAGACTGGAAAGTGTCTTTGCAGGAA1560.1724576318029561No Hit
CGACGAGTCGCGAGTGTGAGCGTGATCAGTAGAAACAAGGTCGTTTTTAA1380.1525586742872304No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGGAAGAATATCAACATCCACAGCAC1320.14592568844865517No Hit
CGACGAGTCGCGAGTGTGAGCGAAGACCACTGTGGACCATATGGCCATAA1260.13929270261007992No Hit
CGACGAGTCGCGAGTGTGAGCGAAAACATAATGGACTCCAACACCATGTC1260.13929270261007992No Hit
CGACGAGTCGCGAGTGTGAGCGCTACGCGTGATCAGTAGAAACAAGGTCG1230.13597620969079233No Hit
CGACGAGTCGCGAGTGTGAGCGCAGAGGCCATGGCCTCTGCTGCCTGTTC1100.1216047403738793No Hit
CGACGAGTCGCGAGTGTGAGCGGAGGGAAGAATATCAACATCCACAGCAC1080.11939374509435423No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGCAGAAATTTGAGGAAATAAGCTGA1020.11276075925577898No Hit
CGACGAGTCGCGAGTGTGAGCGAGTAGAAACAAGGTAGTTTTTTACTCTA1020.11276075925577898No Hit
CGACGAGTCGCGAGTGTGAGCGCTACGCGTGATCAGTAGAAACAAGGTAT1010.11165526161601647No Hit
CGACGAGTCGCGAGTGTGAGCGTGATCAGTAGAAACAAGGTAGTTTTTTA1000.11054976397625392No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGGCAGAAATTTGAGGAAATAAGCTG990.10944426633649137No Hit
CGACGAGTCGCGAGTGTGAGCGGTAGAAACAAGGTAGTTTTTTACTCTAG990.10944426633649137No Hit
CGACGAGTCGCGAGTGTGAGCGGGCAGCAGAGGCCATGGAGGTTGCTAAT990.10944426633649137No Hit
CGACGAGTCGCGAGTGTGAGCGGTCAGTAAGTATGCTAGAGTCCCGTTTT980.10833876869672883No Hit
CGACGAGTCGCGAGTGTGAGCGCTAAGACCACTGTGGACCATATGGCCAT970.10723327105696628No Hit
CGACGAGTCGCGAGTGTGAGCGGTAGGAACAAGGTAGTTTTTTACTCTAG960.10612777341720377No Hit
CGACGAGTCGCGAGTGTGAGCGGGGGGGCCAAGGAGGTGTCACTAAGCTA950.10502227577744122No Hit
CGACGAGTCGCGAGTGTGAGCGCTACGCGTGATCAGCAAAAGCAGGTAAA920.10170578285815358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG156.598976E-494.996688
AGTAGCG156.598976E-494.996686
CACGAGT156.598976E-494.996689
ACGAGTA156.598976E-494.996683
GAGTAGC156.598976E-494.996685
GACGAAT156.598976E-494.996682
CGAGTCA156.598976E-494.996684
GCCGCGA156.598976E-494.996687
AATCGCG156.598976E-494.996686
AGTCACG156.598976E-494.996686
GTAGCGA156.598976E-494.996687
AGGAGTC156.598976E-494.996683
GGAGTCG156.598976E-494.996684
CGACGAG90200.094.20331
GAGTCGC89450.094.147075
CGAGTCG89400.094.14664
GTCGCGA89350.094.146137
ACGAGTC89600.094.095493
AGTCGCG89300.094.092476
CGCGAGT89150.094.0909359
Produced by FastQC (version 0.11.5)