FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC060CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC060CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8010
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGCGAGACAGTCGTGTCGAAAACATAATGGACTCCAACACCATGTC310.38701622971285893No Hit
CGCGAGCGAGACAGTCGTGTCGAACCGAGGTCGAAACGTACGTTCTTTCT260.32459425717852686No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGGACAAAAACATAATGGACTCCAAC250.3121098626716604No Hit
CGCGAGCGAGACAGTCGTGTCGTCAACATCCACAGCACTCTGCTGTTCCT230.28714107365792757No Hit
CGCGAGCGAGACAGTCGTGTCGCGCAGAGACTGGAAAGTGTCTTTGCAGG230.28714107365792757No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGAGGAATCCAGCGAGACACTTAGA210.2621722846441948No Hit
CGCGAGCGAGACAGTCGTGTCGCAGGGGACAAAAACATAATGGACTCCAA210.2621722846441948No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGACAAAAACATAATGGACTCCAACA180.22471910112359553No Hit
CGCGAGCGAGACAGTCGTGTCGCAGACCCGCACTCGCGAGATACTCACTA180.22471910112359553No Hit
CGCGAGCGAGACAGTCGTGTCGGAAAGAGGAATCCAGCGAGACACTTAGA180.22471910112359553No Hit
CGCGAGCGAGACAGTCGTGTCGACAAAAACATAATGGACTCCAACACCAT150.18726591760299627No Hit
CGCGAGCGAGACAGTCGTGTCGCAGCCCCGCACTCGCGAGATACTCACTA140.17478152309612985No Hit
CGCGAGCGAGACAGTCGTGTCGGAAAGGTAACGCGAAGTAGGTACAGATG140.17478152309612985No Hit
CGCGAGCGAGACAGTCGTGTCGATCAGCAAAAGCAGGTAGATATTTAAAG130.16229712858926343No Hit
CGCGAGCGAGACAGTCGTGTCGGAAGAGCACACGTCTGAACTCCAGTCAC120.149812734082397Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CGCGAGCGAGACAGTCGTGTCGAGGGGGCCAAGGAGGTGTCACTAAGCTA120.149812734082397No Hit
CGCGAGCGAGACAGTCGTGTCGCAGACAATGGATTGGGTGATGCCCCATT120.149812734082397No Hit
CGCGAGCGAGACAGTCGTGTCGCGTAGAGACTGGAAAGTGTCTTTGCAGG120.149812734082397No Hit
CGCGAGCGAGACAGTCGTGTCGAAACGCACGTTCTTTCTATCATCCCGTC120.149812734082397No Hit
CGCGAGCGAGACAGTCGTGTCGGAGGGGGGAAAATGGACGAAGGACAAGG120.149812734082397No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGAACATAGTACTGAAAGCGAACTT110.1373283395755306No Hit
CGCGAGCGAGACAGTCGTGTCGACGGACAGAAATTTGAGGAAACAAGGGT110.1373283395755306No Hit
CGCGAGCGAGACAGTCGTGTCGAAGACCACTGTGGACCATATGGCCATAA110.1373283395755306No Hit
CGCGAGCGAGACAGTCGTGTCGCGAGATCGGAAGAGCACACGTCTGAACT110.1373283395755306Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGAGCGAGACAGTCGTGTCGGAAAGGGGAATCCAGCGAGACACTTAGA100.12484394506866417No Hit
CGCGAGCGAGACAGTCGTGTCGATCAGCAAAAGCAGGGTGACAAAAACAT100.12484394506866417No Hit
CGCGAGCGAGACAGTCGTGTCGCGTCCCGCACTCGCGAGATACTCACTAA100.12484394506866417No Hit
CGCGAGCGAGACAGTCGTGTCGCAGAGACTGGAAAGTGTCTTTGCAGGAA100.12484394506866417No Hit
CGCGAGCGAGACAGTCGTGTCGGGCAAGAGAAGAACCCCGCACTCAGAAT90.11235955056179776No Hit
CGCGAGCGAGACAGTCGTGTCGGGCAGGGCAAATATATTCAATATGGAGA90.11235955056179776No Hit
CGCGAGCGAGACAGTCGTGTCGACAAAATGACCATCGTCAACATCCACAG90.11235955056179776No Hit
CGCGAGCGAGACAGTCGTGTCGGCACAGAGAAAGCTCTTATCTCTTGTTC90.11235955056179776No Hit
CGCGAGCGAGACAGTCGTGTCGAAACAAGGAAGTTTTTTACTCTAGCTCT90.11235955056179776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAG7750.095.04
CGCGAGC7900.095.01
CGAGCGA7800.094.999993
GCGAGCG7800.094.999992
AGACAGT7800.094.391029
GCGAGAC7700.094.383126
GAGACAG7700.094.383128
CGAGACA7750.093.774197
AGCGAGA7850.093.184715
GTGTCGC1900.047.50000416-17
GTCGCGG205.2295043E-447.50000418-19
TCGGCAA205.2295043E-447.50000420-21
GTCGAGG205.2295043E-447.50000418-19
GTCGACG205.2295043E-447.50000418-19
GTCGAAG205.2295043E-447.50000418-19
GTCGAAC403.792593E-947.50000418-19
CGAACAG205.2295043E-447.50000420-21
CGCAGGG205.2295043E-447.50000420-21
AGGTCGT205.2295043E-447.50000432-33
TGGGCCT205.2295043E-447.50000476-77
Produced by FastQC (version 0.11.5)