FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC059CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC059CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAGTCGACGCAGCTGTCGGGGGGCGACACGACTGTCTCGCTCGCGA22.380952380952381No Hit
CGCGTAGTCGACGCAGCTGTCGGTTGGCCGACACGACTGTCTCGCTCGCG22.380952380952381No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGGGCGACACGACTGTCTCGCTTGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTCCCGACACGACTGTCTCGCTCGCGAG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGCCGCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGCCTTTCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGGCGTGGTTGGCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTGTGGCCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGTTGTCAGTAAGTATGCTAGAGTCCCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGTTCCGACACGACTGTCTCGCTCGCGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGTGTCAGTAAGTATGCTAGAGTCCCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGCCCCTCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGGGGGCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTGGGCCCCTCGACACGACTGTCTCGCT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGCCCCCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTGTTAAAAAGAGCCTGGTGCCTCCTTC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCTTTAAATCTGTGCCGCATTTCTTCACT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGCAGGCAGGATTTATGTGCAACTGATC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGGCTGAAACGGCTTTCTCTGCCTCC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAAT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGACCCCCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTGGGCCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGACGACGCAGCTGTCGTGGCGCGACACGCCTGGCTCGCTCGCGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGGGGCCGCGACACGACTGTCTCGCTCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGCACATGCTTTTTCTAGATGCTTAT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGGCGACACGACTGTCTCGCTCGGAG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCTTTTAATCTGTGCCGCATTTCTTCAAT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTCCCCGGCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGGGCGCGACACGACTGCCTCGCTAG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGCCCGACACGACTGTCTCGCTCGCGAG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCCTCTTCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGGGGCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTGTGCCCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGATGCTAGAGTCCCGTTTTCGTTCCCT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTCATGGTACCGGACAGCCTCCCTCTCAG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGCCGGCCCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTTCCGGCCCCGACACGACTGTCTCGCT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGCACACACACAAAGTACTTTCTCCCTT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCCCCCCCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCGTTCCCCCCCCCGACCCGACTGTCTCG11.1904761904761905No Hit
CGCGTGGTCGACGCAGCTGTCGGGTGGGAGGTGTTTGGGTCATGGGGCTA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGCGACACGACTGTCTCGCTCGCGAGAT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCAGCCTTCAGTCTATGGCCAAAGGCCCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCGGGTGCGGGACTGCGACATTAGATCTC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGTCCCATAGAAAATCACTGTCGTGTCT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTCCGACACGACTGTCTCGCTCGCGAGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTTTGGCCCTCCGACACGACTGTCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGAAGGATGCTAGAGTCCCGTTTTCCTT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTAAGTATGCTAGAGTCCCGTTTTCGTT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTTGCCCTTGCCGACACGACTGTCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCGGGCCGACACGACTGTCTCGCTCGCGG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTTCCCCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGGGCCTGCCGACACGACTGTCTCGCTC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTCCTTCGACACGACTGTCTAGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGAGTGTACCATGGGTTCTTCATTCTGTC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTAGAAACAAGGCGTTCTGACTCACAAA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGTTTCGACACGACTGTCTCGCTCGCGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTCTGGCTTTCATCTTTCTCCTATGCTG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGGCGACAGAGAATAGTTTCGAACAA11.1904761904761905No Hit
CGCGTGGTCGACGCAGCTGTCGGGGCCGACACGACTGTCTCGCTCGCGAG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTTTCAATCTGTGCCGCATTTCTTCAAT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGTGGCGCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGGGCCCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGCGACACGACTGTCTCGCTCGCGAGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTTTGTATGCTAGAGTCCCGTTTTCGTT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGCGACACGACTGTCTCGCTCGCGCGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGGCTCCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGGAGACTATCATCACCACGCTGATGCT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTTCGACACGACTGTCTCGCTCGCGAGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTCCAGCTTTATCTCATGCTACTGTCCT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGCGCGACACGACTGTCTCGCTCGCGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGCCAAACCTAACAAGGCTAGTCTTACTTT11.1904761904761905No Hit
CGCGTAGTCGAGGCAGCTGTCGGGTCACGACACGACTGTCTCGCTCGCGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGGTGTCCGACACGACTGTCTCGCTCGC11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTCGGCCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGGTGGACGCGACACGACTGTCTCGCTCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGGTCGGATCAAGAAAGAAGAGTTCTCTGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGATCAGTAGAAACAAGGTGATCTTCCA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTCCGGGCTGCGACACGACTGTCTCGCT11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGGCCCGACACGACTGTCTCGCTCGCGA11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTGTGGCCGACACGACTGTCTCGCTCGCG11.1904761904761905No Hit
CGCGTAGTCGACGCAGCTGTCGTTCCGACACGACTGTCTCGCTCGCGAGA11.1904761904761905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC50.094.999998
CGTAGTC50.094.999993
GTCGACG50.094.999997
TAGTCGA50.094.999995
AGTCGAC50.094.999996
CGACGCA50.094.999999
GCGTAGT50.094.999992
CGCGTAG50.094.999991
GTAGTCG50.094.999994
Produced by FastQC (version 0.11.5)