FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC050CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC050CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99558
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGTACACACTACGTAGCAAGGCGATCTTCCAGGAGCTAATGGTAGT7990.8025472588842685No Hit
CGAGAGTACACACTACGTAGCAACCTCCATGGCCTCTGCTGCCTGTTCAC3740.37566041905221076No Hit
CGAGAGTACACACTACGTAGCAAGAAGCTCAAAAGAGAAATAACGTTCCA2280.2290122340746098No Hit
CGAGAGTACACACTACGTAGCAGGAGCTAATGGTAGTGCCACCATCCAGG1530.15367926233954077No Hit
CGAGAGTACACACTACGTAGCACCCCAAGATCGAAGGTTCCAGGTTCCAG1360.13660378874625845No Hit
CGAGAGTACACACTACGTAGCAAGCCCATACACACAAGCAGGCAGGCAGG1320.13258603025372145No Hit
CGAGAGTACACACTACGTAGCACGGATATAAAGAATCAGGCCAAACCTAA1190.11952831515297616No Hit
CGAGAGTACACACTACGTAGCATGGGGCCAAGGAGGTGTCACTAAGCTAT1180.1185238755298419No Hit
CGAGAGTACACACTACGTAGCACTGCCCAATTAGCACATTAGCTTTCTCT1170.11751943590670764No Hit
CGAGAGTACACACTACGTAGCAATTCGACACTAATTGATGGCCATCCGAA1160.1165149962835734No Hit
CGAGAGTACACACTACGTAGCAAGGAGCTTGCTTCTGTAGTGCTGTGTTG1150.11551055666043915No Hit
CGAGAGTACACACTACGTAGCAAAGGGGAGAAGTTTTATGTTCATTCCTT1120.11249723779103638No Hit
CGAGAGTACACACTACGTAGCAAGGCCCTCTTTTCAAACCGTATTTAAAG1120.11249723779103638No Hit
CGAGAGTACACACTACGTAGCACACGTCTGAACTCCAGTCACACAGTGAT1090.10948391892163362TruSeq Adapter, Index 5 (100% over 32bp)
CGAGAGTACACACTACGTAGCACTCCCATTCGCTTCTGGTAGGCCTGCAA1090.10948391892163362No Hit
CGAGAGTACACACTACGTAGCACTGGAGCTAGGATGAGTCCCAATAGTTC1080.10847947929849937No Hit
CGAGAGTACACACTACGTAGCAATGCCCAATTAGCACATTAGCTTTCTCT1010.10144840193655959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGT201.718897E-595.189231
CGTGAGT201.718897E-595.189231
GAGTATA450.094.997994
AGCGTAC201.7361635E-594.9979863
GGTACAC500.094.9979865
GTACACG156.600585E-494.9979866
GAGTACT301.2205419E-894.9979864
CACGCTA156.600585E-494.9979869
TGAGTAC201.7361635E-594.9979863
TACGCAC254.5900924E-794.9979867
GGAGTAC301.2205419E-894.9979863
CGCACTA201.7361635E-594.9979869
AGTACTC301.2205419E-894.9979865
GCGTACA201.7361635E-594.9979864
TACACGC156.600585E-494.9979867
GTACGCA254.5900924E-794.9979866
CGAGAGT94850.094.687451
GAGTACA95000.094.5984
GAGAGTA95350.094.5496442
AGAGTAC95200.094.548943
Produced by FastQC (version 0.11.5)