FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC047CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC047CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36063
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCATAGTGTACGTCGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTT2250.6239081607187422No Hit
CGCTCATAGTGTACGTCGACTCAGGCCAAACCTAACAAGGCTAGTCTTAC1440.39930122285999503No Hit
CGCTCATAGTGTACGTCGACTCAGGCCCTCCTTCCGTAGAAGGCCCTCTT1380.38266367190749523No Hit
CGCTCATAGTGTACGTCGACTCAGGCGCTCCTTCCGTAGAAGGCCCTCTT1200.3327510190499958No Hit
CGCTCATAGTGTACGTCGACTCCAGAGCAGAAATGAAAAGTGGCGAGAGC890.2467903391287469No Hit
CGCTCATAGTGTACGTCGACTCAGTAGAAACAAGGTAGTTTTTTACTCTA880.24401741396999693No Hit
CGCTCATAGTGTACGTCGACTCGAGGCCAAGGGTGTTGCCTCTTCCTTTT730.20242353658874745No Hit
CGCTCATAGTGTACGTCGACTCTGGTTGTTAGTCCATTGTAATACTTTGC700.19410476111249758No Hit
CGCTCATAGTGTACGTCGACTCCACCCCAGATGTGCCTTCATCTGGATCT700.19410476111249758No Hit
CGCTCATAGTGTACGTCGACTCAGGCTCTCCTTCCGTAGAAGGCCCTCTT700.19410476111249758No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGCGAATGGGAGTGCAGATGCAGC670.18578598563624768No Hit
CGCTCATAGTGTACGTCGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGA650.18024013531874775No Hit
CGCTCATAGTGTACGTCGACTCCAGTCACACAGTGATCTCGTATGCCGTC570.158056734048748TruSeq Adapter, Index 5 (96% over 33bp)
CGCTCATAGTGTACGTCGACTCCGGAAGCGAATGGGAGTGCAGATGCAGC520.1441921082549982No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGGGAATGGGAGTGCAGATGCAGC490.1358733327787483No Hit
CGCTCATAGTGTACGTCGACTCCCGCTATATCCTGACCACTCATTTATTC460.1275545573024984No Hit
CGCTCATAGTGTACGTCGACTCAGAAGCGAATGGGAGTGCAGATGCAGCG450.12478163214374843No Hit
CGCTCATAGTGTACGTCGACTCGAGAAGCGAATGGGAGTGCAGATGCAGC440.12200870698499847No Hit
CGCTCATAGTGTACGTCGACTCCAGCGCAAAATGCCATAAGCACCACATT440.12200870698499847No Hit
CGCTCATAGTGTACGTCGACTCCAACGCCATGTCAAGCTTTCAGGTAGAC440.12200870698499847No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGCAAATGGGAGTGCAGATGCAGC430.11923578182624851No Hit
CGCTCATAGTGTACGTCGACTCATTCCCATTTAGGGCATTTTGGATAAAG430.11923578182624851No Hit
CGCTCATAGTGTACGTCGACTCAGGAAACAGGTTTGATCTGATTAAATAC420.11646285666749853No Hit
CGCTCATAGTGTACGTCGACTCTCTGGTTGTTAGTCCATTGTAATACTTT420.11646285666749853No Hit
CGCTCATAGTGTACGTCGACTCCCGAAGCGAATGGGAGTGCAGATGCAGC410.11368993150874859No Hit
CGCTCATAGTGTACGTCGACTCAGTAGAAACAAGGTCGTTTTTAAACAAT400.11091700634999861No Hit
CGCTCATAGTGTACGTCGACTCAGCACCGTCTGGCCAAGACCAACCCACA380.10537115603249869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAT35050.095.383511
TAGTGTA35400.094.9930657
GTGTACG35350.094.993069
AGTGTAC35350.094.85878
TCATAGT35300.094.858514
ATAGTGT35400.094.7247246
CTCATAG35350.094.724343
CATAGTG35500.094.591685
GCTCATA35400.094.590552
TCATAGG255.2081923E-575.994454
ATAGGGT200.002058363871.2448046
CATAGGG200.002058363871.2448045
TAGGGTA200.002058363871.2448047
GGGTACG250.00498345556.9958429
TGCTCAT405.140574E-447.8282131
GCAAGCG205.35637E-447.49653670-71
CTCCGGC404.132744E-947.49653620-21
CTCCGGA205.35637E-447.49653620-21
CTCCGAA205.35637E-447.49653620-21
ATGGATT205.35637E-447.49653688-89
Produced by FastQC (version 0.11.5)