FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC046CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC046CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130320
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACTACGACTGCACGTACAAGGCACCAAACGATCATATGAACAAATG4790.36755678330263963No Hit
CGAGACTACGACTGCACGTACAAAAACATAATGGACTCCAACACCATGTC4760.3652547575199509No Hit
CGAGACTACGACTGCACGTACAATGAACTGGCGACAGTTGAATAGATCGC4710.3614180478821363No Hit
CGAGACTACGACTGCACGTACAACACCATGTCAAGCTTTCAGGTAGACTG4180.32074892572130137No Hit
CGAGACTACGACTGCACGTACACGAAGAAATAAGACCCTTCATTACTCAT4060.3115408225905464No Hit
CGAGACTACGACTGCACGTACAATGACCGAAATTTCTGGAGGGGTGAAAA3800.29158993247391035No Hit
CGAGACTACGACTGCACGTACACAGAGCAGAAATGAAAAGTGGCGAGAGC3370.25859422958870476No Hit
CGAGACTACGACTGCACGTACAAGAAGCTCAAAAGAGAAATAACGTTCCA3080.23634131368938No Hit
CGAGACTACGACTGCACGTACAGAGGGGGGAAAATGGACGAAGGACAAGG3030.23250460405156537No Hit
CGAGACTACGACTGCACGTACAATGCCAAGGAAATTGGAAACGGCTGCTT2950.22636586863106198No Hit
CGAGACTACGACTGCACGTACACGCCCCTGGAAGGACAAATCTTCTGGCT2940.22559852670349909No Hit
CGAGACTACGACTGCACGTACAGAAAGCACCATCCTCTCTATTGTTATGC2740.21025168815224063No Hit
CGAGACTACGACTGCACGTACACCACAAAAGGATATGCTGCTCCCGCTAG2640.20257826887661143No Hit
CGAGACTACGACTGCACGTACAGGGGGGAAAATGGACGAAGGACAAGGGT2630.20181092694904848No Hit
CGAGACTACGACTGCACGTACAAGCCCATACACACAAGCAGGCAGGCAGG2580.19797421731123388No Hit
CGAGACTACGACTGCACGTACACCGCCCCTGGAAGGACAAATCTTCTGGC2480.19030079803560468No Hit
CGAGACTACGACTGCACGTACACCAAGCAGGCAGGCAGGATTTATGTGCA2300.17648864333947206No Hit
CGAGACTACGACTGCACGTACAATGAGCTGGCGACAGTTGAATAGATCGC2260.17341927562922038No Hit
CGAGACTACGACTGCACGTACAGATGCAATTGTCGTTCTAAGTGTCTCGC2230.1711172498465316No Hit
CGAGACTACGACTGCACGTACACACAGCAGGCAGGCAGGATTTATGTGCA2200.16881522406384286No Hit
CGAGACTACGACTGCACGTACAAGGCCCTCTTTTCAAACCGTATTTAAAG2180.167280540208717No Hit
CGAGACTACGACTGCACGTACACTTCTCAATAAAGCAAGATATCGTAGGA2150.16497851442602823No Hit
CGAGACTACGACTGCACGTACAATACCCTGGAACTAAGAAGCAGATACTG2130.1634438305709024No Hit
CGAGACTACGACTGCACGTACAAGCACCACATTCCCTTATACTGGAGATC1940.14886433394720688No Hit
CGAGACTACGACTGCACGTACACATCCAGAAACTGATTGCCCCCAGGGAG1910.14656230816451812No Hit
CGAGACTACGACTGCACGTACAAGAAGCAGATACTGGGCCATAAGGACCA1820.13965623081645182No Hit
CGAGACTACGACTGCACGTACACCGCGCTACCTTTCTGACATGACCCTCG1750.13428483732351137No Hit
CGAGACTACGACTGCACGTACACAAATCCTAAAATTCCCTTAGTCAGAGG1720.1319828115408226No Hit
CGAGACTACGACTGCACGTACACTGCGCGCTACCTTTCTGACATGACCCT1590.1220073664825046No Hit
CGAGACTACGACTGCACGTACAATGCGATAACACGTGCATGGAAAGTGTC1540.11817065684469No Hit
CGAGACTACGACTGCACGTACAATTAGCACATTAGCTTTCTCTCCTTTTG1490.11433394720687538No Hit
CGAGACTACGACTGCACGTACAATTCGACACTAATTGATGGCCATCCGAA1480.11356660527931248No Hit
CGAGACTACGACTGCACGTACAGGGGGGCAAAAACATAATGGACTCCAAC1480.11356660527931248No Hit
CGAGACTACGACTGCACGTACAGAAACGAGAAAGCTCTTATCTCTTGTTC1460.11203192142418662No Hit
CGAGACTACGACTGCACGTACAATTCGCTTCTGGTAGGCCTGCAAATTTT1440.11049723756906078No Hit
CGAGACTACGACTGCACGTACAATAACTTCTCAATAAAGCAAGATATCGT1430.10972989564149785No Hit
CGAGACTACGACTGCACGTACAAGAAACTGATTGCCCCCAGGGAGACTAC1400.10742786985880909No Hit
CGAGACTACGACTGCACGTACAAAAGCAGAAAGCACCATCCTCTCTATTG1360.10435850214855741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT254.5664092E-795.108341
CGTGACT450.095.108341
CGAGAGT600.095.108341
AGACTAA201.7381117E-594.998853
CGAGTGC254.5978777E-794.998859
TAGACTA254.5978777E-794.998852
GAGTACG600.094.998854
AAGACTA700.094.998852
GAATACG301.2234523E-894.998854
CTGCGAC301.2234523E-894.998856
ACTAGGA254.5978777E-794.998855
TACGATT156.604969E-494.998857
TACGAGT254.5978777E-794.998857
CTACGAG254.5978777E-794.998856
TACGACG301.2234523E-894.998857
TGCGACT301.2234523E-894.998857
ACTACAA254.5978777E-794.998855
CTACAAC254.5978777E-794.998856
GCTACGA201.7381117E-594.998855
TAGGACT301.2234523E-894.998857
Produced by FastQC (version 0.11.5)