FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC045CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC045CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173319
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGTCTCGACGTGTGTGTAGTAGCCATTTGTCTGTGAGACCGATGC8470.48869425740974737No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGGTGTT6930.39984075606252056No Hit
CGTGTCGTCTCGACGTGTGTGTAGTCCAAAGTTCTTTCATTTTCCAATAG6370.36753039193625625No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGAGTTTAAAATGAATCCAAA6220.3588758301167212No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGAGTTTAAAATGAATC6000.34618247278140307No Hit
CGTGTCGTCTCGACGTGTGTGTAGAAGCTTTTTGCTCCAGCATGAGGACA5400.3115642255032628No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGGTGACAAAAACATAATGGA5030.29021630634840956No Hit
CGTGTCGTCTCGACGTGTGTGTATCAGCAAAAGCAGGAGTTTAAAATGAA4810.2775229490130915No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGGTGACAAAAACATAA4470.2579059422221453No Hit
CGTGTCGTCTCGACGTGTGTGTATCAGCAAAAGCAGGGTGACAAAAACAT4130.2382889354311991No Hit
CGTGTCGTCTCGACGTGTGTGTTCGGGTCCCCATTCCCATTTAGGGCATT3900.22501860730791198No Hit
CGTGTCGTCTCGACGTGTGTGTGGCAGGGGAAAACAAAAGCAACAAAAAT3870.223287694944005No Hit
CGTGTCGTCTCGACGTGTGTGTAGGACCATGAGCTTGCTGTGGAGAGTGA3670.21174827918462485No Hit
CGTGTCGTCTCGACGTGTGTGTCTGACAAGTTGTATTGCAATCGTGGACT3590.20713251288087287No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGTAGATATTTAAAGATGAGT3390.19559309712149273No Hit
CGTGTCGTCTCGACGTGTGTGTCCCAGTCCATCCGTTCGGATCCCAAATC3320.19155430160570971No Hit
CGTGTCGTCTCGACGTGTGTGTCTGACCAGTTGTATTGCAATCGTGGACT3220.18578459372601963No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGTAGATATTTAAAGAT3190.18405368136211264No Hit
CGTGTCGTCTCGACGTGTGTGTGAGGGGGGAAAATGGACGAAGGACAAGG3130.1805918566342986No Hit
CGTGTCGTCTCGACGTGTGTGTTTTTTATCATTAAATAAGCTGAAACGAG3120.1800148858463296No Hit
CGTGTCGTCTCGACGTGTGTGTCTGCAAAAGCAGGAGTTTAAAATGAATC3070.17713003190648458No Hit
CGTGTCGTCTCGACGTGTGTGTAGCAGGGGAAAACAAAAGCAACAAAAAT3030.17482214875460855No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGCAAAAGCAGGGTGACA3020.17424517796663955No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGTAGTT3020.17424517796663955No Hit
CGTGTCGTCTCGACGTGTGTGTTGTTTTCATAAGTAATGACGCTTTGATT3000.17309123639070154No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGCAAAAGCAGGAGTTTA2990.17251426560273253No Hit
CGTGTCGTCTCGACGTGTGTGTAGGCCAAAGTTCTTTCATTTTCCAATAG2920.1684754700869495No Hit
CGTGTCGTCTCGACGTGTGTGTAGCGGGGGAAAACAAAAGCAACAAAAAT2870.1655906161471045No Hit
CGTGTCGTCTCGACGTGTGTGTTTTCATAAGTAATGACGCTTTGATTGCA2740.1580899959035074No Hit
CGTGTCGTCTCGACGTGTGTGTTACTGTTACATTCTTTTCTAGTACTGTG2640.15232028802381736No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGAGTTT2600.15001240487194134No Hit
CGTGTCGTCTCGACGTGTGTGTGCAGGGGAAAACAAAAGCAACAAAAATG2300.13270328123287117No Hit
CGTGTCGTCTCGACGTGTGTGTGGGGAGCGCCAGGATGCCACAGAAATCA2280.13154933965693316No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGGTAGATAATCACTCA2240.12924145650505714No Hit
CGTGTCGTCTCGACGTGTGTGTAAGACCTGCTGTTGCATCTTCGCCATTG2240.12924145650505714No Hit
CGTGTCGTCTCGACGTGTGTGTAAGTATGCTAGAGTCCCGTTTTCGTTTC2210.12751054414115015No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGGTAGATAATCACTCAATGA2180.12577963177724313No Hit
CGTGTCGTCTCGACGTGTGTGTTTATAGCACCCTTGGGTGTCTGACAAGT2170.12520266098927413No Hit
CGTGTCGTCTCGACGTGTGTGTACGCGTGATCAGTAGAAACAAGGGTATT2110.12174083626146008No Hit
CGTGTCGTCTCGACGTGTGTGTAGGACCAAAGAAGGAAGACGGAAAACAA2040.11770204074567706No Hit
CGTGTCGTCTCGACGTGTGTGTCGGCAAAAGCAGGAGTTTAAAATGAATC2030.11712506995770805No Hit
CGTGTCGTCTCGACGTGTGTGTCTGCAAAAGCAGGGTGACAAAAACATAA1980.11424021601786302No Hit
CGTGTCGTCTCGACGTGTGTGTGAGACCGATGCTGTGAATCAGCAATCTG1900.10962444971411096No Hit
CGTGTCGTCTCGACGTGTGTGTGGGGAGGGAACTTCACCAATAGGACAGC1840.10616262498629694No Hit
CGTGTCGTCTCGACGTGTGTGTATCAGCAAAAGCAGGTAGATATTTAAAG1820.10500868341035893No Hit
CGTGTCGTCTCGACGTGTGTGTCGATTCGAGCCATGCCAGTTATCCCTGC1810.10443171262238993No Hit
CGTGTCGTCTCGACGTGTGTGTAGTCCCAAGTTCTTTCATTTTCCAATAG1790.10327777104645192No Hit
CGTGTCGTCTCGACGTGTGTGTTGTTCGGGTCCCCATTCCCATTTAGGGC1760.1015468586825449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG254.6048444E-794.9971169
ATGTCGT409.094947E-1294.9971162
CTCGACT254.6048444E-794.9971169
GTCTTGA201.739898E-594.9971167
CGTCGCG301.2259989E-894.997116
CGACTCG156.609162E-494.997116
TTCGACG156.609162E-494.997119
CGTGTCG171200.094.437251
CTCGACG171650.094.3606649
TCTCGAC171300.094.359368
GTCTCGA171850.094.278497
TCGTCTC171800.094.195335
CGTCTCG171750.094.19516
GTCGTCT172100.094.1415254
TGTCGTC172500.093.950773
GTGTCGT172450.093.78522
GGTCTCG500.085.4974066
GTCGCGA353.5739504E-881.4260947
TCGTCTG353.5739504E-881.4260945
CGGGTCG301.3394529E-679.393321
Produced by FastQC (version 0.11.5)