FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC044CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC044CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138404
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGACGATCGACGCGCTGAAACGAGAAAGCTCTTATCTCTTGTTCT9650.6972341839831219No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGAGTTTAAAATGAA3330.24059998265946073No Hit
CGAGTAGACGATCGACGCGCTGCATCTGCACTCCCATTCGCTTCTGGTAG3170.22903962313227944No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGTAGATATTTAAAG2750.19869367937342852No Hit
CGAGTAGACGATCGACGCGCTGGGGGGGGGAAAATGGACGAAGGACAAGG2600.18785584231669605No Hit
CGAGTAGACGATCGACGCGCTGATGAGAGAAGAAATAAATACCTAGAAGA2530.18279818502355424No Hit
CGAGTAGACGATCGACGCGCTGACAAAATGACCATCGTCAACATCCACAG2380.17196034796682175No Hit
CGAGTAGACGATCGACGCGCTGTGGGGAGCGCCAGGATGCCACAGAAATC2190.158232421028294No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGGTGACAAAAACAT2180.15750989855784517No Hit
CGAGTAGACGATCGACGCGCTGATTAGCAAAAGCAGGAGTTTAAAATGAA2160.1560648536169475No Hit
CGAGTAGACGATCGACGCGCTGGGGGGAGCGCCAGGATGCCACAGAAATC2070.14956215138290802No Hit
CGAGTAGACGATCGACGCGCTGCACAGATTGAAAGCGACAGAGAATAGTT2030.14667206150111267No Hit
CGAGTAGACGATCGACGCGCTGAACAGCAAAAGCAGGAGTTTAAAATGAA1940.1401693592670732No Hit
CGAGTAGACGATCGACGCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTC1940.1401693592670732No Hit
CGAGTAGACGATCGACGCGCTGAGCAGAAATGAAAAGTGGCGAGAGCAAT1670.12066125256495477No Hit
CGAGTAGACGATCGACGCGCTGGAGGGGGGAAAATGGACGAAGGACAAGG1660.11993873009450594No Hit
CGAGTAGACGATCGACGCGCTGAAGAGCACACAGAATGCCATTGACAAGA1640.11849368515360827No Hit
CGAGTAGACGATCGACGCGCTGAAAACAGAATGGTGCTGGCTAGCACTAC1580.11415855033091529No Hit
CGAGTAGACGATCGACGCGCTGGGGGGGGAAAATGGACGAAGGACAAGGG1560.11271350539001762No Hit
CGAGTAGACGATCGACGCGCTGATAAGCAAAAGCAGGAGTTTAAAATGAA1540.11126846044911996No Hit
CGAGTAGACGATCGACGCGCTGAGGCCATGGAGGTTGCTAATCAGACTAG1510.10910089303777348No Hit
CGAGTAGACGATCGACGCGCTGATGCAATTGTCATTCTAAGTGTCTCGCT1490.10765584809687581No Hit
CGAGTAGACGATCGACGCGCTGATTAGCAAAAGCAGGTAGATATTTAAAG1480.10693332562642698No Hit
CGAGTAGACGATCGACGCGCTGGTGGGAGCGCCAGGATGCCACAGAAATC1480.10693332562642698No Hit
CGAGTAGACGATCGACGCGCTGAACAGAGGTTATAAGAATGATGGAAAGT1470.10621080315597814No Hit
CGAGTAGACGATCGACGCGCTGAGCAGCAAAAGCAGGAGTTTAAAATGAA1430.10332071327418284No Hit
CGAGTAGACGATCGACGCGCTGATTAGCAAAAGCAGGGTGACAAAAACAT1420.102598190803734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG201.7097644E-595.3179861
TGATCGA201.7376662E-595.007969
ACTATCG156.603325E-495.007958
CGAGTAG135900.095.002361
AGACTAT156.6128204E-494.9736256
GTAGGCG156.6128204E-494.9736254
TAGCCGA156.6128204E-494.9736255
GTGGACG201.7407887E-594.9736254
GTAGCCG156.6128204E-494.9736254
GGACGAT201.7407887E-594.9736256
TAGGCGA156.6128204E-494.9736255
AGTAGGC156.6128204E-494.9736253
CGATCGA135900.094.8331769
TAGACGA136250.094.69485
GACGATC136150.094.693937
GTAGACG136700.094.626244
AGACGAT136550.094.556326
AGTAGAC137250.094.350853
GAGTAGA137250.094.2816542
ACGATCG136900.094.140468
Produced by FastQC (version 0.11.5)