FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC035CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC035CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131189
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTT16371.2478180335241522No Hit
CGAGATGACGAGACGCACGACGAAAACATAATGGACTCCAACACCATGTC5460.416193430851672No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATTGCATCTGTACCTA5390.4108576176356249No Hit
CGAGATGACGAGACGCACGACGATCAGCAAAAGCAGGGTGACAAAAACAT5190.3956124370183476No Hit
CGAGATGACGAGACGCACGACGCAAAGAGGGCCTATTATCTTTTGCCTAG5030.3834162925245257No Hit
CGAGATGACGAGACGCACGACGATCAGCAAAAGCAGGTAGATATTTAAAG4660.3552127083825626No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGTAGTTTTT4430.3376807506726936No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATCGCACGCAAAGAGG4410.33615623261096583No Hit
CGAGATGACGAGACGCACGACGAATAGCATAACAATAGAGAGGATGGTGC4150.3163374978085053No Hit
CGAGATGACGAGACGCACGACGATCAGCAAAAGCAGGAGTTTAAAATGAA4000.30490361234554725No Hit
CGAGATGACGAGACGCACGACGATAAGACAGGCAGTTGTGGTCCAGTATC3770.28737165463567826No Hit
CGAGATGACGAGACGCACGACGATCATATGAACAAATGGAGACTGGTGGG3510.2675529198332177No Hit
CGAGATGACGAGACGCACGACGACACCATGTCAAGCTTTCAGGTAGACTG3210.24468514890730167No Hit
CGAGATGACGAGACGCACGACGGAGGGGGGAAAATGAGGTCTTCAATCTC2920.2225796370122495No Hit
CGAGATGACGAGACGCACGACGCAGAGACTGGAAAGTGTCTTTGCAGGAA2850.21724382379620244No Hit
CGAGATGACGAGACGCACGACGAAGAGCATCCCAGTGCTGGGAAGGACCC2580.19666282996287798No Hit
CGAGATGACGAGACGCACGACGAAAAGGGAAAGATAGTCAAATCAGTCGA2450.18675346256164768No Hit
CGAGATGACGAGACGCACGACGAAGAAATAAGACCCTTCATTACTCATGT2430.18522894449991997No Hit
CGAGATGACGAGACGCACGACGGCAAAGGCTATGGAACAGATGGCTGGAT2140.16312343260486778No Hit
CGAGATGACGAGACGCACGACGATTCGAGCCATGCCAGTTATCCCTGCAC2050.15626310132709298No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGCAAAAGCAGGTAGATATTT2020.15397632423450136No Hit
CGAGATGACGAGACGCACGACGGAGGGGGGAAAATGGACGAAGGACAAGG2020.15397632423450136No Hit
CGAGATGACGAGACGCACGACGCTTCGCGTTACCTTTCTGACATGACCCT2000.15245180617277362No Hit
CGAGATGACGAGACGCACGACGGAAAAGGGAAAGATAGTCAAATCAGTCG2000.15245180617277362No Hit
CGAGATGACGAGACGCACGACGATCAGATGAACAAATGGAGACTGGTGGG1990.15168954714190977No Hit
CGAGATGACGAGACGCACGACGATCACATGAACAAATGGAGACTGGTGGG1960.14940277004931818No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTATTTTT1940.14787825198759041No Hit
CGAGATGACGAGACGCACGACGATTCAAATGTGAAGAACTTGTATGAAAA1920.14635373392586268No Hit
CGAGATGACGAGACGCACGACGAAGAGCACACAGAATGCCATTGACAAGA1810.13796888458636014No Hit
CGAGATGACGAGACGCACGACGAATCCAGAAAGCACCATCCTCTCTATTG1720.13110855330858534No Hit
CGAGATGACGAGACGCACGACGAAGCGAATGGGAGTGCAGATGCAGCGAT1710.13034629427772146No Hit
CGAGATGACGAGACGCACGACGCAGAGCAGAAATGAAAAGTGGCGAGAGC1640.12501048106167437No Hit
CGAGATGACGAGACGCACGACGATCAAATGAACAAATGGAGACTGGTGGG1620.12348596299994664No Hit
CGAGATGACGAGACGCACGACGAAAAGGGGAAGATAGTCAAATCAGTCGA1590.12119918590735503No Hit
CGAGATGACGAGACGCACGACGATTCACATGTGAAGAACTTGTATGAAAA1580.12043692687649116No Hit
CGAGATGACGAGACGCACGACGATTATATGAACAAATGGAGACTGGTGGG1570.1196746678456273No Hit
CGAGATGACGAGACGCACGACGGGGGGCAGAAATTTGAGGAAATAAGGTG1570.1196746678456273No Hit
CGAGATGACGAGACGCACGACGAGCAGGGGAAAACAAAAGCAACAAAAAT1530.11662563172217183No Hit
CGAGATGACGAGACGCACGACGATTCGAATGTGAAGAACTTGTATGAAAA1480.11281433656785249No Hit
CGAGATGACGAGACGCACGACGATTCGCTTCTGGTAGGCCTGCAAATTTT1460.11128981850612475No Hit
CGAGATGACGAGACGCACGACGGGTGGGGAGCGCCAGGATGCCACAGAAA1440.10976530044439703No Hit
CGAGATGACGAGACGCACGACGAACAGAGGTTATAAGAATGATGGAAAGT1410.10747852335180541No Hit
CGAGATGACGAGACGCACGACGGTGGGGAGCGCCAGGATGCCACAGAAAT1410.10747852335180541No Hit
CGAGATGACGAGACGCACGACGATCAGTAATCCACAATATCAGGTGCAAG1380.1051917462592138No Hit
CGAGATGACGAGACGCACGACGCCCCCAGGGAGACTACCAGTACCAATGA1380.1051917462592138No Hit
CGAGATGACGAGACGCACGACGATAAATACATTTGAAAAAAAGACGATCA1360.10366722819748608No Hit
CGAGATGACGAGACGCACGACGAGAAAGCTCTTATCTCTTGTTCTACTTC1350.10290496916662221No Hit
CGAGATGACGAGACGCACGACGGGGGGGGCAATAGTTGGAGAAATTTCAC1340.10214271013575832No Hit
CGAGATGACGAGACGCACGACGGGGGACAGAAATTTGAGGAAATAAGGTG1330.10138045110489446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATG128050.095.103971
ATGTCGA156.60538E-494.997725
GATGGCG156.60538E-494.997724
ATGACGT156.60538E-494.997725
GATGACG129400.094.814184
GAGATGA128850.094.7765352
AGATGAC129050.094.740063
CGAGACG130000.094.413129
ATGACGA130300.094.049925
TGACGAG130200.094.0491946
ACGAGAC132650.092.455378
GACGAGA135100.090.8841867
CGTGATG500.085.693971
TGAGATG353.5106495E-881.6133041
AGACGAC301.3607751E-679.1647643
GATGTCG200.002070167871.248284
GTCGAGA200.002070167871.248287
CGTGACG200.002070167871.248289
GTGATGA603.6379788E-1271.248282
TCGAGAC200.002070167871.248288
Produced by FastQC (version 0.11.5)