FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC026CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC026CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59411
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTCTAGATACTCTGTAGAGAAAAGCGAAAGCATAGAAAGTGAGTGTG5940.9998148491020181No Hit
CGCGTCTAGATACTCTGTAGAGAAAAACCAAAGTTTAATGATATGGGTCC4330.7288212620558483No Hit
CGCGTCTAGATACTCTGTAGAGAAGAGAAAGGCACCACACACTTTTAGAC2910.48980828466108967No Hit
CGCGTCTAGATACTCTGTAGAGCCCCGATTGGGCCAAAAGAACCAAAACC2850.4797091447711703No Hit
CGCGTCTAGATACTCTGTAGAGCTACCAAATGTTCGCGAATCATTGGGAA1770.29792462675262155No Hit
CGCGTCTAGATACTCTGTAGAGACCCATTAGAGCACATCCAGAAACTGAT1650.2777263469727828No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCCAAAGTTTAATGATATGGGTCC1210.20366598778004072No Hit
CGCGTCTAGATACTCTGTAGAGCTGCCAAATGTTCGCGAATCATTGGGAA1180.19861641783508105No Hit
CGCGTCTAGATACTCTGTAGAGCCGAGATCAAACCATTGCACTCCAGCCT1150.19356684789012135No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGTGGCACGATGAAA1140.19188365790846812No Hit
CGCGTCTAGATACTCTGTAGAGCCAAAACCAAAGGCCTTAATCCCATTAT1110.18683408796350845No Hit
CGCGTCTAGATACTCTGTAGAGAAAGGCACCACACACTTTTAGACCACCA980.16495261820201645No Hit
CGCGTCTAGATACTCTGTAGAGGGGGGGAGAAGCTTTCAGTCTCGTACCA960.16158623823871002No Hit
CGCGTCTAGATACTCTGTAGAGAACAAGGGAAGAAGAAAAGCGAAAGCAT870.14643752840383092No Hit
CGCGTCTAGATACTCTGTAGAGCACACGTCTGAACTCCAGTCACACAGTG860.14475433842217772TruSeq Adapter, Index 5 (100% over 32bp)
CGCGTCTAGATACTCTGTAGAGAAAACCAAAGTTTAATGATATGGGTCCA850.1430711484405245No Hit
CGCGTCTAGATACTCTGTAGAGGCAGGGGCAAGAGAGAGAAGAGAAAGGC810.13633838851391156No Hit
CGCGTCTAGATACTCTGTAGAGGAAAAGGGAAAGCATAGAAAGTGAGTGT800.13465519853225832No Hit
CGCGTCTAGATACTCTGTAGAGGCCAAAAGAACCAAAACCAAAGGCCTTA760.12792243860564542No Hit
CGCGTCTAGATACTCTGTAGAGAAGGGGAGAAGAAAAGCGAAAGCATAGA750.1262392486239922No Hit
CGCGTCTAGATACTCTGTAGAGCCGAGATCGCGCAGAGACTGGAAAGTGT740.12455605864233896No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGAGCAAGAGAGAGA690.11614010873407282No Hit
CGCGTCTAGATACTCTGTAGAGCAAGGGGGAAGAAAAGCGAAAGCATAGA680.11445691875241959No Hit
CGCGTCTAGATACTCTGTAGAGAACCGGGGAAGAAGTGGACGTTTGAGTG680.11445691875241959No Hit
CGCGTCTAGATACTCTGTAGAGCAGCAAAAGCAGGAGCAAGAGAGAGAAG680.11445691875241959No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGGTGACAAAAACAT680.11445691875241959No Hit
CGCGTCTAGATACTCTGTAGAGCGGGGGACAAAAACATAATGGACTCCAA680.11445691875241959No Hit
CGCGTCTAGATACTCTGTAGAGAGAAGAGAAAGGCACCACACACTTTTAG660.11109053878911312No Hit
CGCGTCTAGATACTCTGTAGAGAAGAACCAAAGTTTAATGATATGGGTCC660.11109053878911312No Hit
CGCGTCTAGATACTCTGTAGAGGTGGGCGGATCACCTGAGGTCGAGAGTT650.1094073488074599No Hit
CGCGTCTAGATACTCTGTAGAGCGGGGGAGAAGCTTTCAGTCTCGTACCA640.10772415882580666No Hit
CGCGTCTAGATACTCTGTAGAGGGCCGGGGAAGAAGTGGACGTTTGAGTG640.10772415882580666No Hit
CGCGTCTAGATACTCTGTAGAGGGGGCCTGACGGGATGATAGAAAGAACG630.10604096884415344No Hit
CGCGTCTAGATACTCTGTAGAGGTGGCACGATGAAACCTTCTAATTCTGG610.10267458888084699No Hit
CGCGTCTAGATACTCTGTAGAGGACCGGGGAAGAAGTGGACGTTTGAGTG610.10267458888084699No Hit
CGCGTCTAGATACTCTGTAGAGGGGAGAAGAAAAGCGAAAGCATAGAAAG600.10099139889919374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCT353.1832315E-1095.1585161
CGCGTCT57300.095.1585161
CGTCTTG156.587266E-494.998323
CGTCTAA156.587266E-494.998323
GTCTAGC156.587266E-494.998324
GTCTAGA57450.094.998324
CTAAATA156.587266E-494.998326
GCTACTC156.587266E-494.998329
GCGTCTT156.587266E-494.998322
TAGGTAC254.5670458E-794.9983147
CGTCTAG57600.094.9983143
TCTAGTT201.7303406E-594.9983145
TAGTTAC201.7303406E-594.9983147
TGTCTAG409.094947E-1294.9983143
GTGTCTA409.094947E-1294.9983142
ACGTCTA201.7303406E-594.9983142
GCGGCTA254.5670458E-794.9983142
CGGCTAG254.5670458E-794.9983143
GCGTCTA57500.094.915712
TAGATAC57550.094.8332447
Produced by FastQC (version 0.11.5)