FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC025CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC025CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301476
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT131694.368175244463904No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT110393.6616513420637133No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT69412.302339158009261No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTGACAAA59711.9805888362589392No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGTAGATATTTAAAGATGAGT34571.1466916106091363No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTATTTT17990.5967307513699266No Hit
CGTCTATCATACGACTGTCTACAAGATCCCAATGATATTTGCTGCAATGA17700.5871114118536799No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGAGTTTAAA16660.5526144701402433No Hit
CGTCTATCATACGACTGTCTACATTCCCATTTAGGGCATTTTGGATAAAG16100.5340391938330082No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTCGTTTT13550.44945534636256285No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGGTGACAAAAACATAATGGA13260.4398360068463161No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGAGTTTTT13200.43784579867054096No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGAGTTTAAAATGAATCCAAA12440.4126364951107219No Hit
CGTCTATCATACGACTGTCTACACCACAAAAGGATATGCTGCTCCCGCTA11560.3834467751993525No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTAGATAA10190.33800368851915247No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGGAAAACA8630.2862582759489976No Hit
CGTCTATCATACGACTGTCTACAACACCATGTCAAGCTTTCAGGTAGACT7640.253419841048707No Hit
CGTCTATCATACGACTGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGT7450.2471175151587523No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGCAAAAGCAGGTAGATATT7290.2418102933566851No Hit
CGTCTATCATACGACTGTCTACATGCGAACAGAGGTTATAAGAATGATGG6860.2275471347636296No Hit
CGTCTATCATACGACTGTCTACGGGGGCGAAACGTACGTTCTTTCTATCA6840.22688373203837123No Hit
CGTCTATCATACGACTGTCTACAACCGAGGTCGAAACGTACGTTCTTTCT6810.22588862795048362No Hit
CGTCTATCATACGACTGTCTACCAGTACCAATGAACTGGCGACAGTTGAA6560.21759609388475368No Hit
CGTCTATCATACGACTGTCTACCAAGATCCCAATGATATTTGCTGCAATG6260.20764505300587774No Hit
CGTCTATCATACGACTGTCTACCGAGGGCGAAACGTACGTTCTTTCTATC6180.20499144210484416No Hit
CGTCTATCATACGACTGTCTACCGGGGGCGAAACGTACGTTCTTTCTATC6130.20333293529169816No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGTAGAAACAAGGTAGTTTT5720.18973317942390105No Hit
CGTCTATCATACGACTGTCTACCGGGGCGAAACGTACGTTCTTTCTATCA5560.18442595762183392No Hit
CGTCTATCATACGACTGTCTACATGCAATGACGAGAGGATCACTTGAATC5460.18110894399554195No Hit
CGTCTATCATACGACTGTCTACCGAGGTCGAAACGTACGTTCTTTCTATC5220.1731481112924412No Hit
CGTCTATCATACGACTGTCTACCCGACCAGTGAGTACCCTTCCCTTTCAA5160.17115790311666598No Hit
CGTCTATCATACGACTGTCTACCAGGGGCGAATGGGAGTGCAGATGCAGC5160.17115790311666598No Hit
CGTCTATCATACGACTGTCTACGGGGGGAAAATGGACGAAGGACAAGGGT5110.16949939630352No Hit
CGTCTATCATACGACTGTCTACCGAAGAAATAAGACCCTTCATTACTCAT4810.15954835542464407No Hit
CGTCTATCATACGACTGTCTACCAGAGCAGAAATGAAAAGTGGCGAGAGC4520.14992901590839736No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGGGAAAACAAAAGCAACAAA4470.14827050909525136No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGGGTTTT4350.144290092743701No Hit
CGTCTATCATACGACTGTCTACGGGGGGCCAAGGAGGTGTCACTAAGCTA4300.142631585930555No Hit
CGTCTATCATACGACTGTCTACCGAGGCCGAAACGTACGTTCTTTCTATC4240.1406413777547798No Hit
CGTCTATCATACGACTGTCTACCCCCCTTCAATGAAGCCGGCAATGGCCC4140.13732436412848784No Hit
CGTCTATCATACGACTGTCTACCCGAACAACATGGATAGAGCAGTTAAAC4140.13732436412848784No Hit
CGTCTATCATACGACTGTCTACTAACCGAGGTCGAAACGTACGTTCTTTC4110.13632926004060023No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTCAAATAT4100.13599755867797106No Hit
CGTCTATCATACGACTGTCTACGCGTGCTCAGCAAAAGCAGGTAGATATT4090.13566585731534186No Hit
CGTCTATCATACGACTGTCTACAAGGTAGTTTTTTACTCTAGCTCTATGT4070.13500245459008345No Hit
CGTCTATCATACGACTGTCTACCATGTTGTTCGGGTCCCCATTCCCATTT3950.1310220382385331No Hit
CGTCTATCATACGACTGTCTACGCGCGATCAGCAAAAGCAGGTAGATATT3950.1310220382385331No Hit
CGTCTATCATACGACTGTCTACGAGAGGATCACTTGAATCGCTGCATCTG3880.1287001287001287No Hit
CGTCTATCATACGACTGTCTACAACCGATTAGAGACCTTGATACTACTAA3850.12770502461224112No Hit
CGTCTATCATACGACTGTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATA3820.1267099205243535No Hit
CGTCTATCATACGACTGTCTACCCGAGAGCAATTGGGACAGAAATTTGAG3780.12538311507383673No Hit
CGTCTATCATACGACTGTCTACCGAGGGGGAAACGTACGTTCTTTCTATC3680.12206610144754473No Hit
CGTCTATCATACGACTGTCTACAAGTGGCACACACTAGACCAAAAGCAGC3660.12140269872228636No Hit
CGTCTATCATACGACTGTCTACAGACACTGTAGACACAGTACTAGAAAAG3630.12040759463439875No Hit
CGTCTATCATACGACTGTCTACGAGGGCGAAACGTACGTTCTTTCTATCA3580.11874908782125276No Hit
CGTCTATCATACGACTGTCTACCAGAAGCGAATGGGAGTGCAGATGCAGC3530.11709058100810678No Hit
CGTCTATCATACGACTGTCTACCGGGAGCGAATGGGAGTGCAGATGCAGC3460.1147686714697024No Hit
CGTCTATCATACGACTGTCTACCGGGGACGAAACGTACGTTCTTTCTATC3340.11078825511815203No Hit
CGTCTATCATACGACTGTCTACAAAAGCAACAAAAATGAAGGCAATACTA3290.10912974830500605No Hit
CGTCTATCATACGACTGTCTACATCCGATCACAATTGGAAAATGTCCAAA3240.10747124149186006No Hit
CGTCTATCATACGACTGTCTACCAGGTGCAAGATCCCAATGATATTTGCT3220.10680783876660166No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGTAGAAACAAGGGTGTTTT3190.10581273467871405No Hit
CGTCTATCATACGACTGTCTACGCGTGCTCAGTAGAAACAAGGTAGTTTT3190.10581273467871405No Hit
CGTCTATCATACGACTGTCTACGAGGGGGGAAAATGGACGAAGGACAAGG3120.10349082514030966No Hit
CGTCTATCATACGACTGTCTACCCGAGGCCAAGGGTGTTGCCTCTTCCTT3060.10150061696453448No Hit
CGTCTATCATACGACTGTCTACGAGGCCGAAACGTACGTTCTTTCTATCA3040.1008372142392761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT700.095.279151
TGATACG450.094.994697
ATCACAC201.7422175E-594.994696
CTAACAT254.6138848E-794.994694
TATTATA353.2923708E-1094.994695
CATACGG201.7422175E-594.994698
TCATCCG550.094.994697
ATACGAC296550.094.081749
CATACGA296800.094.05058
CGTCTAT298050.094.032421
GTCTATC297700.093.973592
TCATACG297500.093.956937
ATCATAC296950.093.907036
TATCATA298500.093.896765
ATCGTAC950.089.994976
TCGTACG950.089.994977
TATCGTA950.089.994975
GTACGAC950.089.994979
ATAGGAC750.088.661729
CATAGGA750.088.661728
Produced by FastQC (version 0.11.5)