FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC019CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC019CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249123
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATACGTACGTCTCAGTCAGGCCAAACCTAACAAGGCTAGTCTTAC7560.303464553654219No Hit
CGAGTATACGTACGTCTCAGTCACACAGTGATCTCGTATGCCGTCTTCTG5960.23923925129353774RNA PCR Primer, Index 5 (97% over 34bp)
CGAGTATACGTACGTCTCAGTCCAGGGGGGAAGAATATCAACAGGAACAG4210.1689928268365426No Hit
CGAGTATACGTACGTCTCAGTCAGGCCCCCTCAAAGCCGAGATCGCGCAG4200.16859141869678834No Hit
CGAGTATACGTACGTCTCAGTCACCACCAAGGTCATGGTGCTAAGCCATT4110.164978745439No Hit
CGAGTATACGTACGTCTCAGTCCTACGCGTGATCAGCAAAAGCAGGTTCA3730.1497252361283382No Hit
CGAGTATACGTACGTCTCAGTCAAAAGCAGGTAGATATTTAAAGATGAGT3690.1481196035693212No Hit
CGAGTATACGTACGTCTCAGTCCAACACCTTTTTGGATCTAAAAAGTACT3590.1441055221717786No Hit
CGAGTATACGTACGTCTCAGTCAGGCGCCCTCAAAGCCGAGATCGCGCAG3520.1412956651934988No Hit
CGAGTATACGTACGTCTCAGTCCAGCGAGACACTTAGAATGACAATTGCA3400.1364787675164477No Hit
CGAGTATACGTACGTCTCAGTCCAGGAGGGAAGAATATCAACAGGAACAG3250.13045764542013383No Hit
CGAGTATACGTACGTCTCAGTCCACGCGTGATCAGCAAAAGCAGGTTCAA3210.1288520128611168No Hit
CGAGTATACGTACGTCTCAGTCCGGGGGGGAAGAATATCAACAGGAACAG3130.12564074774308273No Hit
CGAGTATACGTACGTCTCAGTCTACGCGTGATCAGCAAAAGCAGGTTCAA3060.12283089076480293No Hit
CGAGTATACGTACGTCTCAGTCCGGGGAAGAATATCAACAGGAACAGCAG2850.11440131982996352No Hit
CGAGTATACGTACGTCTCAGTCCCGCACTCGCGAGATACTCACTAAGACC2780.11159146285168371No Hit
CGAGTATACGTACGTCTCAGTCACGCGTGATCAGCAAAAGCAGGTTCAAA2610.10476752447586132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT850.094.9893655
ACGGACG700.094.9893658
CGAGTAT247350.093.116611
TACGTAC247850.092.747337
TATACGT247250.092.722685
ATACGTA248250.092.6744166
GTATACG248100.092.634734
GAGTATA249700.092.497652
AGTATAC248850.092.431883
CGTACGT249250.091.978679
ACGTACG249900.091.948498
CGAGGAT950.090.352641
GGATACG950.089.9899144
CGAACGT600.087.0735869
ACGAACG600.087.0735868
AGTACGT850.083.814159
TACGGAC800.083.115697
CGTAAGT409.495125E-1083.115699
TACGTAA409.495125E-1083.115697
TATACGG750.082.324115
Produced by FastQC (version 0.11.5)