FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC015CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC015CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118676
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGCTGTCGTCGCGATCTCGGCTTTGAGGGGGCCTGACGGGATG11650.9816643634770299No Hit
CGTCGTACGCTGTCGTCGCGATCAAAGCGTCATTACTTATGAAAACAACA9610.8097677710741852No Hit
CGTCGTACGCTGTCGTCGCGATAAGAGGGCAACCCACAGTGTCACTGTTT8410.7086521284842765No Hit
CGTCGTACGCTGTCGTCGCGATAGGCCCCCTCAAAGCCGAGATCGCGCAG7250.6109070073140315No Hit
CGTCGTACGCTGTCGTCGCGATCGGGTTCGTTGCCTTTTCGTCCGAGAGC5420.4567056523644208No Hit
CGTCGTACGCTGTCGTCGCGATAACCGAGGTCGAAACGTACGTTCTTTCT4440.374127877582662No Hit
CGTCGTACGCTGTCGTCGCGATCGGCAAAAGCAGGAGTTTAAAATGAATC4410.3715999865179143No Hit
CGTCGTACGCTGTCGTCGCGATCAGCAAAAGCAGGGTGACAAAAACATAA3760.31682901344838044No Hit
CGTCGTACGCTGTCGTCGCGATCGGCAAAAGCAGGGTGACAAAAACATAA3630.3058748188344737No Hit
CGTCGTACGCTGTCGTCGCGATCGGCAAAAGCAGGTAGATATTTAAAGAT3560.29997640635006234No Hit
CGTCGTACGCTGTCGTCGCGATCTGCAAAAGCAGGAGTTTAAAATGAATC3220.2713269742829216No Hit
CGTCGTACGCTGTCGTCGCGATCTGCAAAAGCAGGGTGACAAAAACATAA3100.2612154100239307No Hit
CGTCGTACGCTGTCGTCGCGATCCAGCCATCTGTTCCATAGCCTTTGCCG2880.24267754221578078No Hit
CGTCGTACGCTGTCGTCGCGATCAGCAAAAGCAGGAGTTTAAAATGAATC2830.23846439044120124No Hit
CGTCGTACGCTGTCGTCGCGATCAGCAAAAGCAGGTAGATATTTAAAGAT2810.23677912973136941No Hit
CGTCGTACGCTGTCGTCGCGATAGGCCCTCTTTGCGTGCGATTGGACCAG2720.2291954565371263No Hit
CGTCGTACGCTGTCGTCGCGATCCACCCCCCTTCAATGAAGCCGGCAATG2690.22666756547237857No Hit
CGTCGTACGCTGTCGTCGCGATCGGAAGAGCACACGTCTGAACTCCAGTC2400.20223128517981734Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CGTCGTACGCTGTCGTCGCGATCTGCAAAAGCAGGTAGATATTTAAAGAT2270.19127709056591055No Hit
CGTCGTACGCTGTCGTCGCGATTGTGGTAAAATTCAAAGCAGCCGTTTCC2150.18116552630691968No Hit
CGTCGTACGCTGTCGTCGCGATGAGTCTTCTAACCGAGGTCGAAACGTAC2000.1685260709831811No Hit
CGTCGTACGCTGTCGTCGCGATCAGTAGAAACAAGGTAGTTTTTTACTCT1960.16515554956351747No Hit
CGTCGTACGCTGTCGTCGCGATGAGGACCCCAACTGCATTTTTGACATCC1940.16347028885368567No Hit
CGTCGTACGCTGTCGTCGCGATGGAGGGGGAAAATGGACGAAGGACAAGG1880.15841450672419022No Hit
CGTCGTACGCTGTCGTCGCGATTGGTGGTAGTAGCCATTTGTCTGTGAGA1850.15588661565944253No Hit
CGTCGTACGCTGTCGTCGCGATGAAAAGGGAAAGACACCCACGGTCGATT1790.15083083352994708No Hit
CGTCGTACGCTGTCGTCGCGATAGAAGGGGAAAACAAAAGCAACAAAAAT1720.14493242104553575No Hit
CGTCGTACGCTGTCGTCGCGATAGGAGGGGAAAACAAAAGCAACAAAAAT1670.14071926927095624No Hit
CGTCGTACGCTGTCGTCGCGATCTACGCGTGATCAGCAAAAGCAGGGTGA1600.13482085678654487No Hit
CGTCGTACGCTGTCGTCGCGATGGGGGACAAAAACATAATGGACTCCAAC1590.13397822643162896No Hit
CGTCGTACGCTGTCGTCGCGATAGGCACCAAACGATCATATGAACAAATG1540.12976507465704945No Hit
CGTCGTACGCTGTCGTCGCGATAGCAGGGGAAAACAAAAGCAACAAAAAT1470.12386666217263811No Hit
CGTCGTACGCTGTCGTCGCGATAGGACCAGGCGGTCATGGAAAAGAACAT1470.12386666217263811No Hit
CGTCGTACGCTGTCGTCGCGATGGGGGGCAAAAACATAATGGACTCCAAC1360.11459772826856315No Hit
CGTCGTACGCTGTCGTCGCGATACGCGTGATCAGTAGAAACAAGGTAGTT1360.11459772826856315No Hit
CGTCGTACGCTGTCGTCGCGATAACAGGGGAAAACAAAAGCAACAAAAAT1350.11375509791364724No Hit
CGTCGTACGCTGTCGTCGCGATAAGGGGAAAACAAAAGCAACAAAAATGA1340.11291246755873133No Hit
CGTCGTACGCTGTCGTCGCGATATTCCCCAAGACAAGTTCATGGCCCAAT1320.11122720684889952No Hit
CGTCGTACGCTGTCGTCGCGATCTGCACTCCCATTCGCTTCTGGTAGGCC1320.11122720684889952No Hit
CGTCGTACGCTGTCGTCGCGATCGGACCAGGCGGTCATGGAAAAGAACAT1310.11038457649398363No Hit
CGTCGTACGCTGTCGTCGCGATCATCCCGTCAGGCCCCCTCAAAGCCGAG1290.10869931578415182No Hit
CGTCGTACGCTGTCGTCGCGATGAAAAGGGAAAGATAGTCAAATCAGTCG1270.10701405507431999No Hit
CGTCGTACGCTGTCGTCGCGATGAAACGAGAAAGCTCTTATCTCTTGTTC1250.10532879436448818No Hit
CGTCGTACGCTGTCGTCGCGATAAGAGGGGAAAACAAAAGCAACAAAAAT1240.10448616400957228No Hit
CGTCGTACGCTGTCGTCGCGATTTGCAGACAATGGATTGGGTGATGCCCC1230.10364353365465637No Hit
CGTCGTACGCTGTCGTCGCGATGGGAGTGCAGATGCAGCGATTCAAGTGA1230.10364353365465637No Hit
CGTCGTACGCTGTCGTCGCGATCAGTAATCCACAATATCAGGTGCAAGAT1210.10195827294482457No Hit
CGTCGTACGCTGTCGTCGCGATGGTGGGGAGCGCCAGGATGCCACAGAAA1210.10195827294482457No Hit
CGTCGTACGCTGTCGTCGCGATTTGTGGTAAAATTCAAAGCAGCCGTTTC1200.10111564258990867No Hit
CGTCGTACGCTGTCGTCGCGATGGGAGGCTGGTGTTTATAGCACCCTTGG1190.10027301223499276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA201.7210288E-595.180741
CGTCGGA201.7210288E-595.180741
CGTCGAA201.7210288E-595.180741
TGTCGTA156.553447E-495.180741
CGCGGTC201.7355236E-595.020238
GCGGTCG201.7355236E-595.020239
TACGCGG201.7355236E-595.020236
GAACGCT201.7355236E-595.020235
GACGCTG201.7355236E-595.020236
ACGCGGT201.7355236E-595.020237
GGTACGC450.094.9801944
GTCGAAC201.7391623E-594.9801942
CGTAGGC201.7391623E-594.9801944
GTCGTAG201.7391623E-594.9801942
AGTACGC201.7391623E-594.9801944
TCGAACG201.7391623E-594.9801943
GTAGTAC201.7391623E-594.9801942
TAGTACG201.7391623E-594.9801943
GTCGGAC201.7391623E-594.9801942
ACGCTGT117800.094.6975867
Produced by FastQC (version 0.11.5)