FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n01_BC009CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n01_BC009CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155867
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGTAGATATTTAAAGATGAGT6320.40547389761784086No Hit
CGAGCTCTATACGTGTAGTCTCCTAACCGAGGTCGAAACGTACGTTCTTT5940.3810941379509453No Hit
CGAGCTCTATACGTGTAGTCTCAGCAAAAGCAGGTAGATATTTAAAGATG5660.36313010451218025No Hit
CGAGCTCTATACGTGTAGTCTCAGGCCCCCTCAAAGCCGAGATCGCGCAG4790.30731328632744587No Hit
CGAGCTCTATACGTGTAGTCTCAGGCACTCCTTCCGTAGAAGGCCCTCTT4720.30282227796775457No Hit
CGAGCTCTATACGTGTAGTCTCTATGTTGACAAAATGACCATCGTCAACA4660.29897284223087633No Hit
CGAGCTCTATACGTGTAGTCTCAGTAGAAACAAGGTAGTTTTTTACTCTA4580.29384026124837204No Hit
CGAGCTCTATACGTGTAGTCTCCGAAGAAATAAGACCCTTCATTACTCAT4130.26496949322178526No Hit
CGAGCTCTATACGTGTAGTCTCAGGCGCTCCTTCCGTAGAAGGCCCTCTT4060.26047848486209396No Hit
CGAGCTCTATACGTGTAGTCTCAACCGATTAGAGACCTTGATACTACTAA3900.2502133228970853No Hit
CGAGCTCTATACGTGTAGTCTCCATCCCCATTTAGGGCATTTTGGATAAA3560.22839985372144198No Hit
CGAGCTCTATACGTGTAGTCTCAACCGAGGTCGAAACGTACGTTCTTTCT3250.2085111024142378No Hit
CGAGCTCTATACGTGTAGTCTCCATACCCATTTAGGGCATTTTGGATAAA2980.19118864159828572No Hit
CGAGCTCTATACGTGTAGTCTCAGCCGATTAGAGACCTTGATACTACTAA2700.17322460815952062No Hit
CGAGCTCTATACGTGTAGTCTCCGAGGGCGAAACGTACGTTCTTTCTATC2600.1668088819313902No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGAGTTTAAAATGAATCCAAA2510.16103472832607288No Hit
CGAGCTCTATACGTGTAGTCTCATGCCCATTTAGGGCATTTTGGATAAAG2400.15397742947512943No Hit
CGAGCTCTATACGTGTAGTCTCATTCCCATTTAGGGCATTTTGGATAAAG2320.1488448484926251No Hit
CGAGCTCTATACGTGTAGTCTCAGGCCCCCATTCCCATTTAGGGCATTTT2140.13729654128199043No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGGTGACAAAAACATAATGGA2080.1334471055451122No Hit
CGAGCTCTATACGTGTAGTCTCAGTCCCCATTCCCATTTAGGGCATTTTG2060.1321639602994861No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGCTTCTGTGGTCACTGTTCCC2050.13152238767667304No Hit
CGAGCTCTATACGTGTAGTCTCCGTGCCCAGTGAGCGAGGACTGCAGCGT1940.12446508882572963No Hit
CGAGCTCTATACGTGTAGTCTCGGCAGGGGAAAACAAAAGCAACAAAAAT1920.12318194358010355No Hit
CGAGCTCTATACGTGTAGTCTCATTCGCTTCTGGTAGGCCTGCAAATTTT1870.11997408046603836No Hit
CGAGCTCTATACGTGTAGTCTCATTCGCATTTAGGGCATTTTGGATAAAG1840.11804936259759923No Hit
CGAGCTCTATACGTGTAGTCTCCAGCCAGCAATGTTACATTTACCCAAAT1840.11804936259759923No Hit
CGAGCTCTATACGTGTAGTCTCCTTCCTCCCTCATCCCCACTTCACTGAA1760.11291678161509493No Hit
CGAGCTCTATACGTGTAGTCTCTGCGCGATCTCGGCTTTGAGGGGGCCTG1730.1109920637466558No Hit
CGAGCTCTATACGTGTAGTCTCAGCAAAAGCAGGGTGACAAAAACATAAT1720.11035049112384275No Hit
CGAGCTCTATACGTGTAGTCTCAGTAGCAACAAGGTAGTTTTTTACTCTA1670.10714262800977756No Hit
CGAGCTCTATACGTGTAGTCTCATCCCATTTAGGGCATTTTGGATAAAGC1640.10521791014133845No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGAGAAATAACGTTCCATGGGGCCA1640.10521791014133845No Hit
CGAGCTCTATACGTGTAGTCTCGCGTGATCAGTAGAAACAAGGTAGTTTT1610.10329319227289932No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGGTAGATAATCACTCAATGA1580.10136847440446022No Hit
CGAGCTCTATACGTGTAGTCTCCGAGGTCGAAACGTACGTTCTTTCTATC1570.10072690178164717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG301.2259989E-894.988147
TAGACGT156.610386E-494.988148
ATTCGTG156.610386E-494.988149
TATTCGT156.610386E-494.988148
TATACGG500.094.988148
GCTCGAT156.610386E-494.988144
GCACTAT156.610386E-494.988144
ATACGGG450.094.988139
GCGCTAT550.094.988134
TATACGT156050.092.857678
ATACGTG156350.092.8019
CGAGCTC157100.092.1389541
CTATACG158300.091.867867
GAGCTCT157900.091.79982
TCTATAC158000.091.74176
CTCTATA158400.091.719925
GAGCGCT650.087.681352
AGCGCTA650.087.681353
AGCTCTA165850.087.571213
GCTCTAT166850.087.274084
Produced by FastQC (version 0.11.5)