FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36799406
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36681119.967853828944955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA47450.011.4760021
TATACTG88650.011.202075
TACACTG75650.010.9232645
TAGACAG139200.010.8833745
GTATATA153500.010.6897021
TAGGACT56150.010.198474
GTATTAG73750.010.1400651
GTCCTAG40100.09.9582691
GTATAGA75000.09.8742581
GTGTAAG77400.09.7556891
TATACAG103500.09.5247825
TCTATAC67150.09.5006013
GTCTTAG55800.09.238251
TAGACTG62350.09.1843215
TCCTAGG58850.08.9994252
CTAATAC80750.08.977843
GTATTAC60700.08.9709441
GTACTAG30900.08.928751
GTGTAAA114100.08.9085691
CTATACT66750.08.9047194
Produced by FastQC (version 0.11.5)