FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21527982
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC22300.019.833141145
CCGACGC51850.016.081095145
CCTATAC90550.014.1691693
CCGTACC32000.014.0444034
TCCGTAC39200.013.8686693
GTATATA371400.013.6480461
TATACTG197550.013.429685
GTATTAA209300.012.6461631
GTCCTAA112950.012.5836031
GTATTAG151600.012.2454831
GTATTAT229700.012.1228691
CCTACAC109300.012.0700983
GTACGGA33750.012.0323221
GTCCTAT98950.012.0188591
CGTACCA37700.011.5364325
GTATAAG137650.011.4845891
GTCCTAG100700.011.449931
TTATACT229000.011.2687594
GTGTATA278650.011.2684461
CTATACT148200.011.2007954
Produced by FastQC (version 0.11.5)