FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22857208
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC23950.016.044432145
CCGACGC41400.014.360433145
GTATATA446000.013.9661881
TATACTG236100.013.8157535
GTATTAG167200.012.6638631
TCTATAC161000.012.4711423
GTATTAA241250.012.3235591
GTATAGA167500.012.2515551
GTATAGG94200.012.1625821
GTATTAT277650.012.0137821
GTCCTAG114200.012.0009411
CTATACT164300.011.8677654
GTGTATA292450.011.777731
CCTATAC96450.011.5736633
GTATAAG157750.011.445891
TACACTG228350.011.4277175
TATACAG222400.011.4075215
GTCCTAA119000.011.3949981
GTCCTAT107100.011.103861
GTATAAT266900.010.976211
Produced by FastQC (version 0.11.5)