FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8831744
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC12000.022.355524145
GTATAAG56350.014.541211
CCGTACC8551.6370905E-1114.4109369
TGACGCG6101.2762575E-714.263162145
TATACAG82150.014.206655
CCTATAC33050.013.81763
GTATATA146850.013.6285971
GTATTAA84800.013.5106831
TATACTG82400.013.2838235
GTATTAG61150.013.0440441
GTATTAT98200.012.8485411
GTGTATA118650.012.8341641
GTATAGG33200.012.6679391
TCTATAC52650.012.6663933
GTCCTAA43550.012.4878921
TAATACT86200.012.4456624
TATACCG7251.0434687E-611.9982145
TTATACT94600.011.8003024
GTATAGA57150.011.800021
GGATCGT14900.011.6745948
Produced by FastQC (version 0.11.5)