FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20177192
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC47750.016.095613145
GTCCTAG97150.014.6289481
TATACTG210700.013.9331145
TACACTG189950.013.7760785
GTATTAG146000.013.6577651
TCTATAC138900.013.2028723
GTATAGA141850.013.1883411
CCTATAC86700.013.125953
GTATATA389550.013.0483211
CCTACAC104550.012.8261973
GTCCTAT91900.012.7820151
GTGTATA256350.012.1345531
GTGCTAG109900.012.0080791
GCCGACC23550.012.007394145
GTATAGG82850.011.990241
CTATACT143400.011.9292464
CTAATAC152700.011.8672783
CTATACA177150.011.7024034
GTCCTAC68100.011.4994341
TCCTATA158550.011.47584152
Produced by FastQC (version 0.11.5)