FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27012729
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG132350.014.9571091
GTATATA382400.013.8804061
TATACTG223600.013.5514195
GTATAAG200100.013.5167011
GTATTAG209850.013.4761111
TATACAG252450.013.381075
GTATTAT320900.012.6087921
GTGTATA269550.012.5897031
GTATAGA211050.012.1282561
GTGTAGG153450.011.6718331
TCTATAC195150.011.5517993
CCTATAC128750.011.4852563
TATATAC381000.011.32008653
CTATACT185450.011.2182864
GTATAAT277950.011.1917911
TACACTG222300.011.1523645
GTATTAA277750.011.121531
TTATACT283700.010.9359334
GTATATG279900.010.9324771
ATACTGT290150.010.8678536
Produced by FastQC (version 0.11.5)