FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19757872
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA349800.014.9668091
CGACGAA12650.014.32923145
GTATTAG138450.014.1934571
TATACTG203000.013.8198945
GTATAGA138150.012.492171
GTATACG19150.012.1169411
GTATAGG80700.012.0404621
GTATTAT223700.011.89631
TATACCG15850.011.8914235
GTGTATA234900.011.6377821
CTAATAC142100.011.4780263
GTATTAC108800.011.46334651
TAATACT193100.011.3748824
GTCCTAA100300.011.2780891
GTATTAA194800.011.2416451
CCTATAC78400.011.1878883
CTATACT135600.011.1730844
TATATAC352550.011.1238563
GTATAAG123250.011.1195371
TCTATAC131150.011.1098083
Produced by FastQC (version 0.11.5)