FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36307690
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA656250.014.9054571
GTATTAG268300.014.7291781
GTATTAT434400.013.6875821
TATACTG354750.013.6702165
GTATAGA261750.013.2417041
GTATAAG248150.012.8862621
GTATAGG160150.012.8133341
TATACAG351350.012.440825
GTATTAA378700.012.2734141
CCTATAC154750.011.9445823
GTACTAG127650.011.9289211
TATATAC658400.011.8243133
GTGTATA429850.011.8082021
CTAATAC260350.011.6380493
TAGACAG649150.011.5799145
TCTATAC253850.011.5648323
TACACTG335250.011.3517335
GTACGGA55300.011.2765441
ACTGATC312850.011.2364098
GTATAAT409200.011.2345661
Produced by FastQC (version 0.11.5)